Glioma cell exosomes containing mir-124 and preparation method and application thereof

A mir-124, glioma cell technology, applied in the field of glioma cell exosomes and their preparation, can solve the problems of not easy to expand in large quantities, unstable exosome yield and composition, and high cost of cell culture.
CN113082216BActive Publication Date: 2022-07-01JILIN UNIV
11 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JILIN UNIV
Publication Date
2022-07-01

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides miR-124-containing glioma cell exosomes, a preparation method and application thereof, and belongs to the technical field of central nervous system damage repairing drugs. A method for preparing glioma cell exosomes containing miR-124, the supernatant obtained by culturing a glioma cell line is subjected to solid-liquid separation; the separated exosomes and miR-124 are mixed and incubated together to obtain a Glioma cell exosomes of miR‑124. The exosomes prepared above can effectively bind to cholesterol-modified miR-124 and be absorbed by reactive astrocytes, significantly increase the expression of miR-124 in reactive astrocytes, and inhibit astrocytes. Activation and scarring. Therefore, the present invention also provides the application of miR-124-containing glioma cell exosomes in the preparation of a drug for inhibiting astrocyte activation and / or glial scar formation, thereby treating central nervous system injury diseases.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of drugs for repairing damage to the central nervous system, in particular to miR-124-containing glioma cell exosomes and a preparation method and application thereof. Background technique

[0002] Astrocytes (As) are the main components of glial cells in the vertebrate central nervous system. When the central nervous system is injured, As is activated into reactive astrocytes (RAs), which synthesize and secrete extracellular matrix mainly composed of chondroitin and keratan sulfate proteoglycan, and interact with microglia, Macrophages, etc. form dense glial scars, affecting the regeneration, elongation and fusion of axons, and compressing microvessels, affecting local blood supply [1] . The glial scars formed by RAs can form a physical barrier and hinder the migration of transplanted cells, so that the use of neural stem cells, mesenchymal stem cells, embryonic stem cells and other cells to treat central...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More