Lentinus edodes strain suitable for industrial cultivation and molecular identification method thereof

A technology of shiitake mushrooms and a screening method, applied in the field of shiitake mushrooms, can solve the problems of low yield of the first wave, dark mushroom caps, and high proportion of mushroom stalks, and achieve the effects of large quantity, neat fruiting and high biotransformation rate.

Active Publication Date: 2021-07-09
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the technical problems of low edible rate, high proportion of mushroom stalks, poor flower shape, low yield of the first tide, and deep color of mushroom caps in the existing production of shiitake mushrooms, so as to provide an edible mushroom. Lentinus edodes strains with high yield, short stalk, good flower shape, light cap color and high yield in the first tide

Method used

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  • Lentinus edodes strain suitable for industrial cultivation and molecular identification method thereof
  • Lentinus edodes strain suitable for industrial cultivation and molecular identification method thereof
  • Lentinus edodes strain suitable for industrial cultivation and molecular identification method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: The main planted shiitake mushroom 'SK11' was selected as the parent, and 99 hybrid strains were obtained, 82 of which had lock joints. Finally, 24 hybrid strains with good growth and lock joints were screened out for initial screening and fruiting In the experiment, after primary screening and secondary screening, it was found that there was only one hybrid, namely Le0152, whose yield was higher than that of the control and parental strains. At the end of the post-ripening period, the fungus bag surface has nodules, and then a large number of primordia are formed. There are many primordia, and the fruiting is fast, but the mushroom body is small. The hybrid strain was named 'Nongxiang 152'.

[0017] The differences between the 'Nongxiang 152' strain and its parent and control strains were compared by antagonistic reaction method.

[0018] Test basis: Inoculation, culture and observation of the samples to be tested are carried out according to the Agricultur...

Embodiment 2

[0023] Example 2: According to the Agricultural Industry Standard of the People's Republic of China "NY / T 1730-2009 ISSR Method for Identification of the Authenticity of Edible Fungus Species", the DNA difference comparison between this strain and its parent was carried out.

[0024] Test materials: 3 shiitake mushroom strains, among which the strain to be tested is 'Nongxiang 152', and 2 control strains are 'SK11' and 'Nongxiang 2'.

[0025] Inspection method: ISSR test primer sequence (Table 1) main references "ISSR fingerprint analysis and SCAR markers of main cultivated strains of black fungus in China", "ISSR fingerprint analysis of wild black fungus strains in Heilongjiang area", "ISSR analysis of black fungus cultivated strains Analysis", primers were synthesized by Qingke Biotechnology Co., Ltd.

[0026] The mycelium activation, cultivation, genomic DNA extraction, quality inspection, PCR amplification and electrophoresis detection of the strains to be tested are all i...

Embodiment 3

[0034] Example 3: 'Nongxiang 152' has carried out many stable yield tests, and the results show that 'Nongxiang 152' meets the requirements of industrial cultivation, and its yield is higher than that of the control Nongxiang 2.

[0035] Stable production test strains: Nongxiang No. 2, a provincially recognized variety.

[0036] Test location: Fujian Branch Center of National Variety Improvement Center (Youxi, Fujian).

[0037] Test arrangement: The strains are uniformly produced, and the test sites are cultivated and managed in accordance with local production management methods.

[0038] Test results: The test found that the hyphae of 'Nongxiang 152' were white and sparse in PDA, and the growth was slow, and the optimum growth temperature was 22-25 ℃.

[0039]Cultivation findings: (1) The time from inoculation to harvest, "Nongxiang 152" was 7 days shorter than the provincial certified variety Nongxiang 2, and 2 days shorter than the parent 'SK11'; (2) The yield of the firs...

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Abstract

The invention discloses a lentinus edodes strain Nongxiang 152, which belongs to lentinus edodes and is preserved in the China General Microbiological Culture Collection Center. The Nongxiang 152 strain provided by the invention has the advantages that the fruiting is early, the fruiting can be realized without color change, the upper part and the lower part of the stipe are equal in thickness, and no villus exists. The method has the quality characteristics of high-quality shiitake mushroom strains, such as single mushroom body, tidy fruiting, large quantity, high biotransformation rate, centralized fruiting of one tide, and the like. The mushroom body color and the biological conversion rate (yield) of the parent SK11 are improved, the fruiting period is 7 days earlier than that of a control variety Nongxiang No.2, and fruiting can be achieved without color changing. The method has the advantages of concentrated yield, short cultivation period and the like of the parent SK11, has excellent comprehensive characters, is particularly suitable for industrial cultivation due to concentrated fruiting of the first tide, and has a good popularization prospect.

Description

technical field [0001] The invention belongs to the field of shiitake mushrooms, and in particular relates to a shiitake mushroom strain Nongxiang 152 suitable for industrial cultivation and a molecular identification method thereof. Background technique [0002] Shiitake mushroom [Lentinus edodes (Berk.)sing] belongs to Agaricia, Agaricidae, Agaricinae, Agaricales, Agaricaceae, and Lentinus genus, and is a common edible fungus in the market. Mushrooms are sold in two ways, dried mushrooms and fresh mushrooms, according to market requirements. The most commonly eaten parts are the caps of shiitake mushrooms. Fresh shiitake mushrooms can be dehydrated to make dried shiitake mushrooms, which is convenient for transportation and storage. Dried and fresh shiitake mushrooms are widely used in Chinese cuisine and are an important north-south product. At present, low water content, moderate thickness of mushroom cap, moderate opening degree, short mushroom legs and good mushroom s...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N1/14A01H15/00C12Q1/6895C12Q1/04C12R1/645
CPCA01H15/00C12Q1/6895C12Q2600/156
Inventor谢宝贵刘芳王瑞清蔡鹏岑光红赖丽红王圣铕刘新锐吴小平
OwnerFUJIAN AGRI & FORESTRY UNIV