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Screening and identification method of human umbilical cord-derived Muse cells

An identification method, the technology of human umbilical cord, applied in the field of cell research, can solve rare problems

Inactive Publication Date: 2021-07-13
HENAN YINFENG BIOENG CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the culture of Muse cells in umbilical cord-derived mesenchymal stem cells, and there are few reports on the screening and identification of the cells.

Method used

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  • Screening and identification method of human umbilical cord-derived Muse cells

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Embodiment Construction

[0025] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0026] see figure 1 , a method for screening and identifying human umbilical cord-derived Muse cells, comprising the following steps:

[0027] Step 1. Identify the participants.

[0028] Step 2: Determine the experimental equipment and reagents.

[0029] Step 3, sorting of Muse cells, sorting primary cultured dermal fibroblasts SSEA-3 positive cells by flow cytometry, and cultivating Muse cell clusters under the condition of MC medium and poly-HEMA coated pl...

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Abstract

The invention discloses a screening and identification method of human umbilical cord-derived Muse cells. The method comprises the following steps: determining participants, determining experimental instruments and reagents, sorting the Muse cells, performing in-vitro amplification on the Muse cells, and identifying the Muse cells. The method disclosed by the invention has the beneficial effects that positive cells of the primarily cultured dermal fibroblasts SSEA-3 are sorted by a flow cytometer, the Muse cell cluster is cultured under the conditions of an MC culture medium and a poly-HEMA coated plate, and the self-renewal capacity of suspension-adherence-suspension and proliferation amplification of the Muse cell cluster is observed. The cells can be differentiated into adipocytes and osteoblasts through bone and adipogenesis induction of the third generation Muse cells. It is proved that a human umbilical cord source Muse cell culture scheme is successfully established in the project.

Description

technical field [0001] The invention relates to a method for screening and identifying Muse cells derived from human umbilical cord, in particular to a method for screening and identifying Muse cells derived from human umbilical cord, and belongs to the technical field of cell research. Background technique [0002] With the development of stem cell technology in the treatment of diseases, people are increasingly aware of the role of stem cell technology in promoting human health. In recent years, it has been discovered that Muse cells derived from mesenchymal stem cells have the characteristics of strong anti-stress ability, good homing effect, and low tumorigenicity in stem cell transplantation, which has opened up a new direction for the further improvement of stem cell therapy technology. Umbilical cord mesenchymal stem cells are rich in sources, easy to obtain, non-invasive to the donor, easy to separate and culture and expand in vitro, less ethical restrictions, and mo...

Claims

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Application Information

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IPC IPC(8): C12N5/0775C12N5/077C12Q1/02
CPCC12N5/0653C12N5/0654C12N5/0668C12N2509/00G01N33/5005
Inventor 郭晓丹程志远马晴陈少辉
Owner HENAN YINFENG BIOENG CO LTD
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