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Method for extracting, purifying and detecting volatile oil of creeping rostellularia herb and application of volatile oil of creeping rostellularia herb

A purification method and volatile oil technology, which is applied in the research and development of pharmaceutical products, can solve the problems of less acanthus, achieve low cost, improve extraction rate, and prevent the coking of raw materials

Pending Publication Date: 2021-07-16
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few studies on Acanthus; there is no report on the extraction and use of the volatile oil of Acanthus

Method used

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  • Method for extracting, purifying and detecting volatile oil of creeping rostellularia herb and application of volatile oil of creeping rostellularia herb
  • Method for extracting, purifying and detecting volatile oil of creeping rostellularia herb and application of volatile oil of creeping rostellularia herb
  • Method for extracting, purifying and detecting volatile oil of creeping rostellularia herb and application of volatile oil of creeping rostellularia herb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1 Extraction of Extraction of Volatile Oil of Judge

[0076] (1) Take 500 g of fresh 床 床 粹 粹 粹 目 目 目 目 进行 进行 进行 进行 进行 进行; 进行 进行;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;

[0077] (2) EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc EtOAc.

Embodiment 2

[0078] Example 2 Test Method for Volatile Oil of Juji Bulk

[0079]It was detected by GC-MS to detect the rushing oil obtained by Embodiment 1. The specific test conditions are as follows: HP-5MS column (30m × 0.25 mm × 0.25 μm); carrier gas: helium; flow rate: 1.2ml / min, solvent Delayed 5 min; heating program: The initial temperature was 60 ° C, maintained at 8 ° C / min to 200 ° C, maintained for 5 min, and then raised to 280 ° C at 5 ° C / min to maintain 2 min. Ionization: EI, 70EV; Iomeical temperature: 250 ° C; carrier gas: helium; column flow rate: 1.2 ml / min. 47 of chemical composition in the volatile oil of the priests were detected, and the specific chemical composition and relative content were shown in Table 2.

[0080] Table 2 床 油 油 成 成

[0081]

[0082]

[0083]

Embodiment 3

[0084] Example 3 Antibacterial effect of volatile oil

[0085] 1, test strain and medium

[0086] Test strains: Select four bacterial strains for antibacterial test, including Gram-positive bacteria: Golden Staphylococcus aureus (ATCC 6538), Bacillus Bacillus (ATCC 6633), Bacillus Bacillus (ATCC 9545) and Gram Macrobacteria: Escherichia coli (ATCC 25922), Pseudomonas (ATCC 27853).

[0087] Test medium: bacterial MHB (Mueller Hinton Broth) medium.

[0088] 2, test method

[0089] (1) Sterilization:

[0090] The medium, test tube, petri dish, filter sheet (6 mm), gun head, distilled water, or the like needed by the experiment were sterilized for 20 min at 121 ° C, and the ultraviolet sterilization of ultraviolet sterilization of ultraviolet workstraps was stored.

[0091] (2) formulation of bacterial suspension:

[0092] From the active bacteria, a cyclocrystis was collected in the corresponding MHB medium, and the conditions were placed in a constant temperature shaker for 37 ° C o...

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Abstract

The invention belongs to the technical field of research and development of medical products, and discloses a method for extracting, purifying and detecting volatile oil of creeping rostellularia herb and application of the volatile oil of creeping rostellularia herb. The volatile oil of creeping rostellularia herb is extracted and purified by adopting steam distillation and organic extraction technologies for the first time; the extraction and purification method is simple in process and low in cost; distilled water and an organic solvent in a preparation process can be recycled; and the volatile oil obtained by the preparation process is low in water content, tests prove that the volatile oil has good bacteriostatic and anticancer activity, and the volatile oil has a good bacteriostatic effect on staphylococcus aureus, bacillus subtilis, escherichia coli and pseudomonas aeruginosa, presents a good antioxidant effect and is suitable for industrial production and application.

Description

Technical field [0001] The present invention belongs to the technical development of pharmaceutical product research and development, and specific to the extraction purification, detection method and application of the volatile oil of Jue. Background technique [0002] The information disclosed in the background is only intended to increase the understanding of the overall background of the present invention, and is not necessarily considered to recognize or imply that the information is intended to be a prior art known to those of ordinary skill in the art. [0003] Volatile Oils, also known as essential oils, is a general term that can be volatilized at room temperature, which can be distilled with water vapor, and the oil-shaped oily liquid is not mixed with water. Most volatile oils have an aromatic odor. Volatile oil is an important active ingredient. Clinical Direct application mainly contains physiciatry of flourishing oil, and can also be applied from the essential volati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02G01N30/02A61K36/19A61P35/00A61P31/04A61P39/06
CPCC11B9/027C11B9/025G01N30/02A61K36/19A61P35/00A61P31/04A61P39/06A61K2236/331A61K2236/333Y02A50/30
Inventor 刘旭赖鹏翔
Owner SHANDONG UNIV
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