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Establishment method and application of animal model with nerve injury induced by HIV infection

A nerve injury, animal model technology, applied in the field of biomedicine, can solve the problem of not acquiring HIV-1 infection, unable to faithfully simulate the initial infection stage or AIDS progression, etc.

Active Publication Date: 2021-07-23
WUHAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, they have significant limitations
Most importantly, they did not acquire HIV-1 infection and thus could not faithfully simulate the initial infection phase or AIDS progression, key events associated with HAND development

Method used

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  • Establishment method and application of animal model with nerve injury induced by HIV infection
  • Establishment method and application of animal model with nerve injury induced by HIV infection
  • Establishment method and application of animal model with nerve injury induced by HIV infection

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Embodiment Construction

[0029] In order to further understand the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. . Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0030] Unless otherwise specified, the reagents involved in the examples of the present invention are all commercially available products, which can be purchased through commercial channels.

[0031] 1. Construction of Chimeric HIV Virus Plasmid

[0032] 1) The MLV envelope replaces the HIV envelope to complete the construction of chimeric HIV; by replacing the HIV-gp120 coding region with the gp80 envelope gene from Mol...

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Abstract

The invention relates to an establishment method and application of an animal model with nerve injury induced by HIV infection. The establishment method comprises a chimeric HIV virus inoculated in the abdominal cavity of a mouse or a chimeric HIV virus injected in the caudal vein of the mouse after the mouse is anesthetized by isoflurane, wherein the chimeric HIV virus has a nucleotide sequence as shown in SEQID NO.1. The animal model can greatly promote mechanism studying on nerve injury of patients caused by HIV, and can be used for pharmaceutical effect determination and pharmacological studying of anti-HIV related nerve injury medicines, especially has a great application value in the aspect of screening of new drugs.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for establishing an animal model of nerve injury caused by HIV infection and its application. Background technique [0002] Although combined antiretroviral therapy (cART) can systematically inhibit HIV virus replication and maintain a very low viral load, most cART drugs cannot cross the blood-brain barrier, causing HIV-related neuropathy. Cognitive impairment (HAND) is still prevalent. With the prolongation of patient survival, about 50% of infected patients will further develop into HIV-associated neurocognitive disorders (HAND), including psychomotor slowing, attention, memory, Motor function, executive function, verbal fluency, information processing speed, sensory / perception. The evolution of HAND is from preclinical asymptomatic neurocognitive impairment (asymptomatic neurocognitive impairment, ANI) to mild neurocognitive disorder (mild neurocogn...

Claims

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Application Information

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IPC IPC(8): A61K35/76C12N7/01A61K49/00A01K67/027
CPCA61K35/76A61K49/0008C12N7/00C07K14/005A01K67/027C12N2740/13022C12N2740/16022A01K2267/03Y02A50/30
Inventor 顾潮江杨思浪何晓宏宋志宏于琪英马妍孙瑜
Owner WUHAN UNIV OF SCI & TECH
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