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Cod skin collagen peptide having antioxidant and immune-enhancing function, and preparation method thereof

A protein peptide and skin collagen technology, which is applied in the preparation methods of peptides, chemical instruments and methods, and medical preparations containing active ingredients, etc. Resource waste and other issues, to achieve high purity and safety, good functional properties, and the effect of enhancing immunity

Active Publication Date: 2021-07-23
ANHUI GUOTAI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Cod skin contains a lot of collagen and has good nutritional value. However, cod skin, as the leftovers of cod processing in aquatic enterprises, is often discarded or used to make low-value products such as fish meal. The comprehensive utilization level is not high, resulting in Waste of massive protein resources and environmental pressure
The collagen peptides rich in cod skin are hydrolyzed by protease, and the collagen peptides of cod skin have good water absorption, oil absorption, water retention and foaming properties. There are reports on beauty and skin care, calcium absorption promotion, etc., but there are few studies on cod skin collagen peptides in anti-oxidation and improving immunity, especially protein peptides with specific effects, which have not been reported yet

Method used

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  • Cod skin collagen peptide having antioxidant and immune-enhancing function, and preparation method thereof
  • Cod skin collagen peptide having antioxidant and immune-enhancing function, and preparation method thereof
  • Cod skin collagen peptide having antioxidant and immune-enhancing function, and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0054] Embodiment 1 has anti-oxidation and the cod skin collagen peptide preparation method (1) of strengthening immunity

[0055] This embodiment provides a method for preparing cod skin collagen peptides with anti-oxidation and immunity-enhancing properties, specifically as follows:

[0056] (1) Select cod skin, put the cod skin in 1.0 times the water that meets the drinking water hygiene standard, cook under high temperature and high pressure at 121°C for 60 minutes, cool to 70°C, and use an ultrasonic generator (60KHz) for 30 minutes to obtain cod skin protein solution;

[0057] (2) regulate protein content in the cod skin protein liquid to be 8%, add protease and carry out step-by-step enzymolysis according to the percentage by weight of protein weight 1.2% in the cod skin protein liquid, the first step enzymolysis adds the compound protease of protein weight 1.0% ( trypsin and neutral protease with a mass ratio of 1:1), and carry out the enzymatic hydrolysis reaction at...

Embodiment 2

[0063] Embodiment 2 has anti-oxidation and the cod skin collagen peptide preparation method (2) of enhancing immunity

[0064] This embodiment provides a method for preparing cod skin collagen peptides with anti-oxidation and immunity-enhancing properties, specifically as follows:

[0065] (1) Select cod skin, place the cod skin in 3.0 times the water that meets the drinking water hygiene standard, cook under high temperature and high pressure at 115°C for 120 minutes, cool to 75°C, and use an ultrasonic generator (75KHz) for 25 minutes to obtain cod skin protein solution;

[0066] (2) regulating the protein content in the cod skin protein liquid is 10%, adding protease according to the percentage by weight of protein weight 1.3% in the cod skin protein liquid and carrying out step-by-step enzymolysis, the first step enzymatic hydrolysis adds the compound protease of protein weight 0.8% ( Trypsin and neutral protease with a mass ratio of 2:1), carry out the enzymolysis reacti...

Embodiment 3

[0072] Embodiment 3 has anti-oxidation and the cod skin collagen peptide preparation method (3) of strengthening immunity

[0073] This embodiment provides a method for preparing cod skin collagen peptides with anti-oxidation and immunity-enhancing properties, specifically as follows:

[0074] (1) Select cod skin, place the cod skin in water 5 times higher than the hygienic standard for drinking water, treat it at 105°C for 180 minutes, cool to 70°C, and use an ultrasonic generator (90KHz) for 30 minutes to obtain cod skin protein liquid;

[0075] (2) regulate protein content in the cod skin protein liquid to be 13%, add protease according to the percentage by weight of protein weight 1.5% in the cod skin protein liquid and carry out step-by-step enzymolysis, the first step enzymolysis adds the compound protease of protein weight 0.9% ( Trypsin and neutral protease with a mass ratio of 3:1), carry out the enzymatic hydrolysis reaction at 45°C for 4 hours; add 0.6% flavor prot...

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Abstract

The invention relates to the technical field of animal food processing, and specifically relates to a cod skin collagen peptide having an antioxidant and immune-enhancing function, and a preparation method thereof. The preparation method of the provided cod skin collagen peptide includes the following steps: taking cod skins as a raw material, and performing two-step enzymolysis on the pre-treated raw material to obtain enzymatic hydrolysate, wherein in the two-step enzymolysis, the enzyme used in first step enzymolysis is compound protease, and the compound protease is trypsin and neutral protease; and the enzyme used in second step enzymolysis is flavourzyme. Thecollagen peptide obtained by the preparation method has high small molecule peptide content, and has an excellent antioxidant and immune-enhancing function.

Description

technical field [0001] The invention relates to the technical field of animal food processing, in particular to a cod skin collagen peptide with anti-oxidation and immune-enhancing functions and a preparation method thereof Background technique [0002] Cod skin contains a lot of collagen and has good nutritional value. However, cod skin, as the leftovers of cod processing in aquatic enterprises, is often discarded or used to make low-value products such as fish meal. The comprehensive utilization level is not high, resulting in Waste of massive protein resources and environmental pressure. The collagen peptides rich in cod skin are hydrolyzed by protease to obtain collagen peptides. At present, the collagen peptides of cod skin have good water absorption, oil absorption, water retention and foaming properties. At the same time, they have There are reports on skin care and calcium absorption, but there are few studies on cod skin collagen peptides in anti-oxidation and impr...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34C07K1/16C07K1/20A61K38/39A61K38/01A61P39/06A61P37/04A23L33/18
CPCC12P21/06C07K14/78A61P39/06A61P37/04A23L33/18A61K38/00A23V2002/00A23V2200/30A23V2200/324Y02P20/10
Inventor 赖圆圆罗永康刘怀高崔景龙衣大龙张恒
Owner ANHUI GUOTAI BIOTECHNOLOGY CO LTD
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