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Enhanced fluorescent carbon dot, preparation method and application in cadmium ion detection

A fluorescent carbon dot and enhanced technology, applied in fluorescence/phosphorescence, chemical instruments and methods, nano-carbon, etc., can solve the problems of high biological toxicity, poor selectivity, and low sensitivity, and achieve low cytotoxicity, simple method, and high selective effect

Active Publication Date: 2022-07-05
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the carbon dots have a short emission wavelength (450nm) and are easily interfered by intracellular autofluorescence. Not only is the tissue penetration ability poor, but the response to cadmium ions is fluorescence quenching, which greatly reduces the sensitivity of intracellular cadmium ion detection.
Therefore, detection of intracellular Cd 2+ There are some problems that need to be solved urgently in practical applications: (1) high biological toxicity; (2) mostly quenching type, low sensitivity and poor selectivity; (3) mostly short-wavelength emission, lack of long-wavelength emission carbon dots

Method used

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  • Enhanced fluorescent carbon dot, preparation method and application in cadmium ion detection
  • Enhanced fluorescent carbon dot, preparation method and application in cadmium ion detection
  • Enhanced fluorescent carbon dot, preparation method and application in cadmium ion detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Grind 0.5 g of salicylic acid and 0.025 g of 5-amino-1,10-phenanthroline to mix well, then transfer the powder to a 25 mL crucible, raise the tube furnace temperature to 200 °C, The heating rate was 5°C / min, and the temperature was kept for 2h. After cooling to room temperature, the product was dissolved in ultrapure water, filtered with a 0.22 μm microporous membrane, the filtrate was put into a 3500Da dialysis bag, dialyzed for 48h, and the outer liquid was freeze-dried to obtain red fluorescent carbon dot powder.

[0050] The obtained red fluorescent carbon dot powder was dissolved in ultrapure water, dropped on an ultrathin copper grid, and its morphology was observed. Transmission electron microscope (TEM) image of carbon dots figure 1 As shown, it can be seen that the prepared carbon dot particles are uniformly dispersed, and the average particle size is 2.46 nm.

[0051] The prepared red fluorescent carbon dots were tested by ultraviolet spectrum, and the resul...

Embodiment 2

[0059] Weigh 0.5g of salicylic acid and 0.015g of 5-amino-1,10-phenanthroline powder and place them in a quartz mortar for thorough grinding. Transfer the uniformly mixed powder to a 25mL quartz crucible for calcination. The furnace temperature was raised to 200 °C for 2 h, and the heating rate was 5 °C / min. After cooling to room temperature, the obtained product was dissolved in ultrapure water, filtered through a 0.45 μm microporous membrane, and the filtrate was collected and dialyzed at 1000 Da for 24 h. Finally, the red fluorescent carbon dot powder is obtained by freeze-drying.

[0060] Using the method of Example 1 to detect the red fluorescent carbon dot powder prepared in this example, it can be found that the prepared red fluorescent carbon dot particles are uniformly dispersed, the maximum excitation wavelength is 450 nm, and the emission peak is located at 605 nm. 2+ High selectivity, strong anti-interference ability, low cytotoxicity, Cd 2+ The concentration of ...

Embodiment 3

[0062] Weigh 0.5g of salicylic acid and 0.05g of 5-amino-1,10-phenanthroline powder and place them in a quartz mortar for sufficient grinding, transfer them to a 25mL quartz crucible for calcination, and raise the temperature of the tube furnace to The reaction was carried out at 220 °C for 4 h, and the heating rate was 5 °C / min. After cooling to room temperature, the obtained product was dissolved in ultrapure water, filtered with a 0.22 μm microporous membrane, and the filtrate was collected and dialyzed at 3500 Da for 36 h. Finally, the red fluorescent carbon dot powder is obtained by freeze-drying.

[0063] Using the method of Example 1 to detect the red fluorescent carbon dot powder prepared in this example, it can be found that the prepared red fluorescent carbon dot particles are uniformly dispersed, the maximum excitation wavelength is 450 nm, and the emission peak is located at 605 nm. 2+ High selectivity, strong anti-interference ability, low cytotoxicity, Cd 2+ Th...

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Abstract

The invention discloses an enhanced fluorescent carbon dot, a preparation method and an application in cadmium ion detection, and belongs to the technical field of preparation of luminescent carbon nanomaterials. In the present invention, enhanced red carbon dots are synthesized by calcining a specific carbon source A and a carbon source B, wherein the carbon source A includes one or more of salicylic acid, phthalic acid and benzoic acid; the carbon source B is The phenanthroline or a derivative thereof, wherein the derivative of the phenanthroline is a derivative with any one or several groups of amino group, carboxyl group or hydroxyl group. The method of the invention is simple and easy to prepare in large quantities. Cd 2+ The addition of , will enhance the fluorescence of the fluorescent carbon dots, and the resulting fluorescent carbon dots are more sensitive to Cd 2+ It has high selectivity, and its fluorescence intensity is similar to that of Cd 2+ The concentration of cadmium ion showed a good linear relationship within 5μM-100μM, the detection limit was 0.304μmol / L, and the fluorescence-enhanced signal was used to detect intracellular cadmium ions.

Description

technical field [0001] The invention relates to an enhanced fluorescent carbon dot, a preparation method and an application in cadmium ion detection, and belongs to the technical field of preparation of fluorescent carbon nanomaterials. Background technique [0002] With the development of modern industry, heavy metal pollution has seriously threatened human health and environmental safety. Among various heavy metal ions, cadmium ion is one of the most serious pollution sources. Cd 2+ It has a half-life of 10-30 years in the human body and is classified as a strong carcinogen. Cd 2+ It can cause serious damage to the human lung, kidney, bone and nervous system, causing bone disease, heart disease and diabetes. Therefore, the detection of cadmium ions is of great significance in disease diagnosis and environmental monitoring. At present, researchers have developed a series of detection methods for cadmium ions, such as cyclic voltammetry, atomic absorption spectroscopy, i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65B82Y20/00B82Y40/00C01B32/15G01N21/64
CPCC09K11/65B82Y20/00B82Y40/00C01B32/15G01N21/6428
Inventor 王婵宋启军徐雅兰黄建锋庞庆丰贺依民姜涛
Owner JIANGNAN UNIV
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