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Application of cordyceps militaris pharmaceutical composition in preparation of preparation for preventing and/or treating novel coronaviruses and resisting respiratory viruses

A kind of anti-respiratory, Cordyceps militaris technology

Pending Publication Date: 2021-08-13
SHANGHAI PUBLIC HEALTH CLINICAL CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, cordycepin is sensitive to adenosine deaminase, and is easily deaminated by adenosine deaminase in the body, especially in the blood circulation[10]

Method used

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  • Application of cordyceps militaris pharmaceutical composition in preparation of preparation for preventing and/or treating novel coronaviruses and resisting respiratory viruses
  • Application of cordyceps militaris pharmaceutical composition in preparation of preparation for preventing and/or treating novel coronaviruses and resisting respiratory viruses
  • Application of cordyceps militaris pharmaceutical composition in preparation of preparation for preventing and/or treating novel coronaviruses and resisting respiratory viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] In view of the problem that there is no effective treatment drug for respiratory virus infection, this embodiment provides a Cordyceps militaris medicinal composition for prevention and treatment of respiratory virus.

[0038] Because the common pathogenic coronaviruses and influenza viruses of the respiratory tract are RNA viruses, the RNA Coxsackie B3 virus (CVB3, Nancy strain, Shanghai Public Health Clinical Center) with no biosafety risk is used as a research model. It has been confirmed horizontally that the Cordyceps militaris extract containing cordycepin and pentostatin can effectively inhibit the replication of CVB3 virus in its susceptible human malignant embryonic rhabdomyoma cells (RD), and protect the cells from the pathogenic effect of the virus. Low concentrations of cordycepin combined with pentostatin also have the same antiviral effect.

[0039] Further, the pharmaceutical composition of Cordyceps militaris provided in this embodiment includes the extr...

Embodiment 2

[0042] This example is an antiviral activity experiment of high concentration cordycepin alone.

[0043] The experimental method includes the following steps:

[0044] Step 1, adjust the concentration of human malignant embryonic rhabdomyoma cells (RD) to 3×10 with DMEM cell culture fluid containing 10% fetal bovine serum (FBS). 5 / mL, uniformly inoculated at 1 mL per well in a 12-well plate and cultured overnight.

[0045] Step 2, after diluting cordycepin with DMEM culture medium, different final concentrations of cordycepin-containing solutions (1ug / mL, 10ug / mL, 20ug / mL, 30ug / mL) were obtained. The experiment was divided into blank control group and experimental group. Treat the RD cells with the blank control group (only containing DMEM culture medium) and the four experimental groups (containing the above four solutions containing cordycepin), and infect the Coxsackie virus type B3 at a ratio of 2 (MOI=2) (CVB3) RNA virus. After 1 hour of virus infection, the virus li...

Embodiment 3

[0049] This example provides an antiviral experiment of low concentration cordycepin combined with pentostatin.

[0050] Experimental method: The experimental method provided in this example is similar to that in Example 2, the only difference is that the experimental group is divided into eight groups, namely: the final concentration of 0.1ug / mL cordycepin alone group, the final concentration of 1ug / mL cordycepin Single use group, final concentration of 5ug / mL cordycepin single use group, final concentration of 30ug / mL cordycepin single use group, final concentration of 3ug / mL pentostatin single use group, final concentration of 0.1ug / mL Cordycepin + 3ug / mL pentostatin combined group, the final concentration is 1ug / mL cordycepin + 3ug / mL pentostatin combined group, the final concentration is 5ug / mL cordycepin + 3ug / mL pentostatin Joint use group.

[0051] Experimental results such as figure 2 shown.

[0052] figure 2 It is the antiviral experiment result of the combinat...

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Abstract

The invention relates to application of a cordyceps militaris pharmaceutical composition in preparation of a preparation for preventing and / or treating novel coronaviruses and resisting respiratory viruses, and belongs to the technical field of medical biology. The cordyceps militaris pharmaceutical composition comprises a cordyceps militaris extracting solution, or cordycepin and pentostatin. The cordycepin and the pentostatin are locally combined for nasopharynx, so that the clinical effect of quickly removing novel coronaviruses can be achieved, medicine reserve can be provided for quickly removing pathogens in the early virus infection stage of respiratory infectious diseases, preventing severe pneumonia and timely blocking epidemic situations; and the problems of drug metabolism loss, drug toxicity and drug tissue accessibility caused by systematic administration can be avoided. In addition, compared with the cordyceps militaris, the combination of the cordycepin and the pentostatin is more efficient in antiviral effect, low in price, mature in chemical synthesis technology and capable of realizing mass production, and has good clinical application prospect and social and economic value.

Description

technical field [0001] The invention relates to the field of medical biotechnology, in particular to the application of a Cordyceps militaris pharmaceutical composition in the preparation of preparations for preventing and / or treating the new coronavirus and anti-respiratory virus. Background technique [0002] Respiratory infections are often caused by coronaviruses or influenza viruses. So far, coronaviruses have caused 3 public health emergencies of international concern [1], and influenza viruses have caused 6 world outbreaks [2]. The population is generally susceptible to coronaviruses and influenza viruses, and the positive rate of neutralizing antibodies can be as high as 50% to 70% [3-5]. Different types of viruses have different pathogenicity. Common coronavirus and influenza virus strains usually cause mild or moderate upper respiratory tract infections, while MERS-CoV, SARS-CoV and SARS-CoV-2 coronavirus infections can develop into Middle East Respiratory Syndro...

Claims

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Application Information

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IPC IPC(8): A61K36/068A61K31/7076A61K9/00A61K9/12A61P31/14A61P11/00A61P31/12A61P31/16A61P11/02A61K31/7056
CPCA61K36/068A61K31/7076A61K31/7056A61K9/0043A61K9/0073A61K9/12A61P31/14A61P11/00A61P31/12A61P31/16A61P11/02A61K2300/00
Inventor 刘铁夫卢洪洲徐丽凌云赵隽张林张丽军宋志刚
Owner SHANGHAI PUBLIC HEALTH CLINICAL CENT
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