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J substock lymphoid leuoosis-resistant polyclonal antibody and preparation method thereof

A polyclonal antibody and avian leukosis technology, applied in the direction of antiviral immunoglobulin and serum immunoglobulin, can solve the problems of accelerated virus mutation and evolution, no subsidy, and unscientific, and reduce direct and indirect economic losses , stable properties and long shelf life

Inactive Publication Date: 2013-06-19
SHANDONG AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0007] (3) Mixed infection increases the frequency of viral recombination
Large-scale comprehensive purification also brought some problems. In addition to a large increase in breeding costs, the most potential impact is to promote the evolution of ALV-J
[0014] Under the current situation, my country basically has no effective prevention and control measures for subgroup J avian leukemia, the government has no subsidies, and no company can afford the high cost of purification. Due to the characteristics of ALV-J itself and my country's The status quo of farming, biosecurity measures have little effect on it
At the same time, unscientific and unreasonable purification measures will also accelerate the mutation and evolution of the virus, making the disease more complicated

Method used

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  • J substock lymphoid leuoosis-resistant polyclonal antibody and preparation method thereof
  • J substock lymphoid leuoosis-resistant polyclonal antibody and preparation method thereof
  • J substock lymphoid leuoosis-resistant polyclonal antibody and preparation method thereof

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Experimental program
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Embodiment Construction

[0031] The production of polyclonal antibodies will be further described in detail in conjunction with the accompanying drawings.

[0032] 1. According to the previous research, the conservative sequence of the gp37 gene was determined to be LQNRAAIDFLLLAQGHGCQDVEGMCCF. According to this sequence, the ISU small peptide was synthesized by Beijing Zhongke Yaguang Biotechnology Co., Ltd. and the small peptide was coupled with KLH and BSA respectively, using high performance liquid chromatography ( The HPLC method was used for synthesis and purification identification, and the purity reached more than 97%. The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method identified a good coupling effect.

[0033] 2. Choose about 1.5 months old New Zealand white rabbits, which are healthy males and weigh about 2Kg. Before immunization, blood is collected from the ear vein to separate serum and stored for later use as a negative control for titer testing. After 7 days ...

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Abstract

The invention relates to a J substock lymphoid leuoosis-resistant polyclonal antibody and a preparation method thereof. The J substock lymphoid leuoosis-resistant polyclonal antibody can effectively inhibit the infection of J substock avian leukosis virus, and is obtained on the basis of highly conserved sequence synthesized small peptides formed by 27 amino acid in transmembrane protein coded with J substock avian leukosis virus gp 37 gene through a coupled keyhole hemocyanin immune rabbit; and the polyclonal antibody blocks the invasive J substock avian leukosis virus reproduction by being combined with the nucleus non-specifity, and is combined with and eliminates the specificity of the J substock avian leukosis virus, thereby effectively preventing the generation and spreading of the J substock lymphoid leuoosis, greatly reducing the economic loss of aviculture caused by infection of J substock avian leukosis virus, and providing a novel method and thinking for the lymphoid leuoosis preventing and controlling.

Description

(1) Technical field [0001] The invention relates to a polyclonal antibody against J subgroup avian leukemia and a preparation method thereof (2) Background technology [0002] Subgroup J avian leukemia is a neoplastic disease caused by subgroup J avian leukemia virus (ALV-J). ALV-J is an enveloped retrovirus, and clinical infection in poultry mainly manifests as myelocytoma, immune tolerance, high mortality and growth retardation. From 1997 to 1998, ALV-J broke out globally, causing a devastating blow to the world broiler breeder industry. In addition to myeloma caused by ALV-J infection, erythroblastoma, hemangioma, nephroma, and sarcoma often occur in the late stage of infection. The mortality rate is usually 1%-5%, and the peak period can reach 50%. Studies have confirmed that ALV-J has a lasting damage to the immune response, resulting in low productivity, frequent secondary infections and mixed infections, and the outbreak of J subgroup avian leukemia will cause great...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/10C07K16/06
Inventor 成子强王晓伟张利王峰
Owner SHANDONG AGRICULTURAL UNIVERSITY
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