Kidney-targeted near-infrared fluorescent probe and its preparation method and application
A near-infrared and luminescent probe technology, applied in the field of fluorescent probes and their preparation, can solve the problems of imaging diagnosis or targeted drug delivery instability, short residence time of probes, fluctuations in fluorescent signals, etc.
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Embodiment 1
[0031] Weigh 20 mg of ICG-COOH into a round-bottomed flask, add 6 mL of dichloromethane to dissolve, then add 15 mg of EDC and 15 mg of NHS, and stir the above reaction solution at room temperature in the dark for 24 h. Subsequently, the reaction solvent was dried by vacuum rotary evaporation to obtain the activated ICG mixture. The above mixture was separated by silica gel column chromatography to obtain the pure product of activated ICG. Weigh 3 mg of the activated product above and dissolve it in 3 mL of dimethylformamide, add 10 mg of triethylamine to adjust the pH of the solution to 8-9, then add 7 mg of KTP1, and place the above reaction in the dark and stir at room temperature for 12 h, The ICG-KTP1 kidney targeting probe mixture was obtained. The solvent dimethylformamide was removed by vacuum rotary evaporation, and the obtained solid powder was redispersed with dichloromethane to obtain a suspension. The supernatant was discarded and the precipitate was washed with ...
Embodiment 2
[0033] Weigh 10 mg of FD1080 into a round bottom flask, add 4 mL of dichloromethane to dissolve, then add 5 mg of EDC and 5 mg of NHS, and place the above reaction solution at room temperature and stir for 12 h in the dark. Subsequently, the reaction solvent was dried by vacuum rotary evaporation to obtain the activated FD1080 mixture. The above mixture was separated by silica gel column chromatography to obtain the pure product of activated FD1080. Weigh and dissolve 2 mg of the activated product above in 2 mL of methanol, add 10 mg of triethylamine to adjust the pH of the solution to 8-9, then add 5 mg of KTP2, and place the above reaction in the dark and stir at room temperature for 12 h to obtain FD1080 - KTP2 kidney targeting probe mix. The solvent methanol was removed by vacuum rotary evaporation, and the obtained solid powder was redispersed with ethyl acetate to obtain a suspension. The supernatant was discarded and the precipitate was washed with ethyl acetate for 3 ...
Embodiment 3
[0035]Weigh gold nanoparticles GNP (particle size 2.5 nm, 3 mg) into a glass bottle, add 5 mL of deionized water to dissolve, then add 5 mg of KTP1 polypeptide, put the above reaction bottle in a water bath at 45 degrees Celsius, and stir for 18 h, Kidney-targeting peptide-coupled gold nanoparticles. The above product was placed in a rotary evaporator to remove water to obtain GNP-KTP1 fluorescent probe powder. The above products were separated by Sephadex G25 chromatographic column to obtain the purified kidney targeting probe GNP-KTP1.
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