Salmonella pullorum and mycoplasma synoviae double-plate agglutination antigen as well as preparation method and application thereof
A technology for Mycoplasma synovialum and Salmonella, applied to biochemical equipment and methods, methods based on microorganisms, bacteria, etc., to achieve good stability, rapid diagnosis, and cost-saving detection effects
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Embodiment 1
[0037] Example 1, Preparation of double plate agglutination antigen of Salmonella pullorum pullorum and Mycoplasma gallinarum
[0038] The double plate agglutination antigen of Salmonella pullorum and Mycoplasma gallinarum mentioned in this example is made of Salmonella pullorum C79-1 and Mycoplasma pullorum GX11-T, Salmonella pullorum strain C79-1 and chicken The Mycoplasma synoviae strains GX11-T are all preserved in the China Center for Type Culture Collection, and the preservation numbers are: CVCC79207 and CVCC2960, respectively.
[0039] Salmonella pullorum antigen was prepared by the following method:
[0040](1) Preparation of seed bacteria liquid: after recovery and propagation of Salmonella pullorum C79-1 strain in TSB liquid medium, inoculate TSA solid plate medium, culture at 37°C for 18 hours, select typical colonies and inoculate TSA solid slant medium, 37 After cultivating at ℃ for 20 hours, wash the bacterial lawn with Martin's liquid medium, expand the cultur...
Embodiment 2
[0048] Example 2, the method of using double plate agglutination antigen of Salmonella pullorum and Mycoplasma gallinarum
[0049] 30 minutes before use, take out the agglutinated antigen, negative or positive serum and the serum to be tested from the double plate of Salmonella pullorum and Mycoplasma gallinarum from the refrigerator, and let it reach room temperature. Use a pipette to absorb a drop (0.025mL-0.05mL) of the well-mixed diagnostic antigen, drop it vertically on a glass plate, and then quickly add a drop (0.025mL-0.05mL) of the same amount of serum to be tested next to the antigen. Mix the serum and antigen evenly, spread it into a sheet with a diameter of 1cm-2cm, shake the glass plate constantly, and observe the results within 2min. The test should be carried out under the condition of 20℃~25℃. Each batch of tests should be set positive serum and negative serum control.
[0050] Judgment of the results: if more than 50% (++) of agglutination occurs within 2 mi...
Embodiment 3
[0054] Example 3, double plate agglutination antigen specificity experiment of Salmonella pullorum and Mycoplasma gallinarum
[0055] The prepared double-plate agglutinated antigens of Salmonella pullorum and Mycoplasma gallinarum were mixed with Salmonella gallinarum positive serum, Mycoplasma gallinarum positive serum, Escherichia coli positive serum, Staphylococcus gallinarum positive blood, Haemophilus paragallinarum positive serum , chicken Newcastle disease positive serum, avian influenza positive serum, chicken infectious bronchitis positive serum, chicken adenovirus positive serum, etc. were subjected to plate agglutination reaction, and the results were as follows image 3 As shown, only the positive serum of Salmonella gallinarum and the positive serum of Mycoplasma synovialum had agglutination reaction, and the others were all negative.
[0056] Example 3, clinical application of double plate agglutination antigen of Salmonella pullorum and Mycoplasma gallinarum
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