Improved method for plasma immobilization of a biomolecule to a substrate via a linking molecule

A technology of plasma and biomolecules, applied in the field of ions, can solve problems such as limiting biomolecules and steric hindrance

Pending Publication Date: 2021-09-03
MOLECULAR PLASMA GRP SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Another disadvantage of this method is the efficiency of immobilization of biomolecules
Simultaneous deposition of linker molecules and biomolecules results in steric hindrance that limits the amount of deposited biomolecules

Method used

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  • Improved method for plasma immobilization of a biomolecule to a substrate via a linking molecule
  • Improved method for plasma immobilization of a biomolecule to a substrate via a linking molecule
  • Improved method for plasma immobilization of a biomolecule to a substrate via a linking molecule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0084] method

[0085] The following buffers were used in these experiments. A mixture of 25 ml (milliliters) of PBS (Dulbecco's phosphate-buffered saline, Gibco) and 0.25 g (grams) of BSA (bovine serum albumin, Sigma-Aldrich) was created. make up the blocking buffer, shake to ensure that each has dissolved. Contain 25ml type I ultrapure water (Synergy UV Milli-Q water, Millipore (Millipore)), 2.5g mannitol (D-mannitol ≥ 98%, Sigma-Aldrich company) and 0.5g sucrose (D( +) - Sucrose 99.7%, storage buffer from Acros Organics. Wash buffer containing 150 ml PBS and 3 μl Tween 80 (Sigma-Aldrich). Care is required due to the viscosity of Tween 80. PTA buffer containing 150 ml PBS, 0.15 g BSA and 3 μl Tween80. Determine the mass of the components using an analytical balance.

[0086] All nozzles were 3D printed specifically for this experiment. via glass bottle 10 ml of PBS was added to the solution, and then 196 μl of goat-derived anti-human IgE antibody (Novex, Invitrogen...

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Abstract

The current invention relates to a two-step method for the immobilization of a biomolecule through a linking molecule on a sample surface of a substrate by generating and maintaining a non-thermal atmospheric pressure plasma at a temperature between room temperature and 60 DEG C. The preferred plasma temperature is room temperature. The method comprises of a first step and second step, which are sequentially carried out. In the first step of the method, the linking molecule is deposited onto the sample surface through exposing the sample surface to a first plasma jet and the linking molecule, generating a linking layer onto the sample surface. In a second sequential step of the method, the biomolecule is deposited onto the linking layer through exposing the linking layer to a second plasma jet and the biomolecule.

Description

technical field [0001] The present invention relates to plasma technology and in particular to the inclusion of biomolecules into plasma deposited layers. Background technique [0002] WO2017 / 136334A1 discloses methods for depositing biomolecules, pharmaceuticals and other therapeutically active agents on surfaces. The method includes a plasma apparatus having a plasma outlet exposed to ambient pressure, one or more electrodes, a gas supply inlet, and an ignition system operatively connected to the electrodes to provide a non-thermal equilibrium plasma within a plasma chamber. Particle delivery systems can be used to introduce the active agent as a dry powder into or downstream of the plasma and deposit the plasma-treated active agent to create a coating on the surface. The coating maintains the activity of the active agent. This invention attempts to solve the problem of eliminating any wet chemical steps to obtain at least two-layer biocoatings on substrates using atherm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C23C24/04C23C4/04C23C4/134C23C16/448
CPCB05D1/36B05D1/62C23C4/04C23C4/134C23C16/4486C23C24/04C23C16/513H05H1/3457B05D2203/35
Inventor G·斯徹尔金R·黑博格
Owner MOLECULAR PLASMA GRP SA
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