A mouse model of immunosuppressive myocarditis and its construction method and application

A mouse model and immunosuppressive technology, applied in the field of applied basic medical research, can solve the problems of dilated cardiomyopathy, heart failure, non-specific treatment methods, non-specific clinical manifestations, etc., and achieve high tolerance in mice. , to avoid potential biosafety hazards, and to be well tolerated by mice

Active Publication Date: 2022-05-17
HUNAN UNIV OF CHINESE MEDICINE
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0002] Myocarditis (Myocarditis) is a common inflammatory disease of the myocardium. The incidence has been on the rise in recent years, and the clinical manifestations are non-specific. It is easy to be ignored and lead to serious consequences such as dilated cardiomyopathy and heart failure. According to statistics, about 21% of myocarditis Patients can develop dilated cardiomyopathy, in which in up to 12% of heart disease cases, myocarditis is thought to be the cause of sudden cardiac death in young people, but there is currently no specific clinical treatment
[0003] At present, animal models of myocarditis at home and abroad mainly include cytomegalovirus-induced and Coxsackie virus-induced mouse models. Domestic myocarditis models mostly use 6-8-week-old male BALB / c mice that are relatively susceptible to Coxsackie virus. Constructing models through viruses has biological safety hazards and high mortality

Method used

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  • A mouse model of immunosuppressive myocarditis and its construction method and application
  • A mouse model of immunosuppressive myocarditis and its construction method and application
  • A mouse model of immunosuppressive myocarditis and its construction method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation of normal control group, positive control group and model group

[0043] 1) Experimental animals

[0044] 25 SPF-grade BALB / c mice, male, 4-6 weeks old, weighing 16-20 g, were purchased from the Experimental Animal Center of Hunan University of Traditional Chinese Medicine and bred in the Experimental Animal Center of Hunan University of Traditional Chinese Medicine [SCXK (Xiang) 2019-0009 】. During the feeding period, the rats in each group drank water freely and were fed with common maintenance feed, which was provided by the Experimental Animal Center of Hunan University of Traditional Chinese Medicine. Breeding environment: Day and night half-cycle lighting, humidity 40-60%, temperature control at 20-25°C, light / dark cycle of 12 hours. All operations complied with the experimental ethics requirements of Hunan University of Traditional Chinese Medicine [LL2020120202].

[0045] 2) Experimental drugs and reagents

[0046] Cyclosporin A (Cyclos-porin A, ...

Embodiment 2

[0059] Example 2 HE staining observation of pathological changes in mouse myocardium and detection of various indicators

[0060] 1) Animal handling

[0061] One day after the last injection, all surviving mice were sacrificed by cervical dislocation for specimen collection. Routinely fix, disinfect, and open the chest of the mice, separate the thymus, spleen, lung, liver, kidney and heart, take out the heart and put it in 4°C pre-cooled normal saline immediately, rinse the residual blood, and then dry it with filter paper , the apical tissue was fixed in conventional 4% paraformaldehyde, and the rest of the tissue was stored at -80° for long-term preservation.

[0062] 2) Thymus index calculation

[0063] The electronic balance weighs the heart, thymus, spleen, and lungs, and then calculates the organ mass index, where organ mass index=organ mass / body mass.

[0064] For the thymus index of the blank control group, model group, and positive control group, see figure 2 . ...

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Abstract

The invention provides an immunosuppressive myocarditis mouse model and its construction method and application. The method comprises the following steps: selecting male BALB / c mice aged 4 to 6 weeks as experimental mice for constructing a mouse model of myocarditis, observing and feeding them for three days; The amount of sporin A solution is 40-50 mg / kg mouse body weight per injection, once every other day, for a total of three injections; one day after the last injection, the mouse model of myocarditis is obtained. The present invention uses cyclosporin A as an immunosuppressant, successfully induces myocarditis and causes myocardial damage according to a specific administration method, and constructs a mouse model of myocarditis without death. Compared with the prior art, the inflammation is induced by virus infection and LPS The mouse model constructed has the advantages of being safe and effective, and the experimental mice are better tolerated.

Description

technical field [0001] The invention belongs to the field of applied basic medical research, in particular to an immunosuppressive myocarditis mouse model and its construction method and application. Background technique [0002] Myocarditis (Myocarditis) is a common inflammatory disease of the myocardium. The incidence rate has been on the rise in recent years, and the clinical manifestations are non-specific. It is easy to be ignored and lead to serious consequences such as dilated cardiomyopathy and heart failure. According to statistics, about 21% of myocarditis Patients can develop dilated cardiomyopathy, in which in up to 12% of heart disease cases, myocarditis is considered to be the cause of sudden cardiac death in young people, but there is currently no specific clinical treatment. [0003] At present, animal models of myocarditis at home and abroad mainly include cytomegalovirus-induced and Coxsackie virus-induced mouse models. Domestic myocarditis models mostly us...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/13A01K67/027
CPCA61K38/13A01K67/027A01K2267/0368A01K2267/0375Y02A50/30
Inventor 魏科赵天浩陈凯琴卢芳国蒋艺轩徐琰喆邱丹
Owner HUNAN UNIV OF CHINESE MEDICINE
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