Anti-fasciola gigantica Cat L1 monoclonal antibody as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of biology, and discloses an anti-fasciola gigantica Cat L1 monoclonal antibody as well as a preparation method and application thereof, and the preparation method of the anti-fasciola gigantica Cat L1 monoclonal antibody comprises the following steps: performing mouse immunization; constructing a hybridoma cell strain; and carrying out monoclonal antibody preparation and sensitivity identification. According to the invention, expression rFgCat L1 is utilized to immunize five BALB / c mice, the mice with the highest antibody reaction are taken, splenocytes are separated and fused with SP 2 / 0 cells, and a positive hybridoma cell strain is constructed. 47 parts of buffalo serum and 47 parts of goat serum which are detected to be positive through an indirect ELISA method in the early stage are detected through the method, and the coincidence rates of positive results reach 72.3% and 78.7%. The anti-rFgCatL1 monoclonal antibody is successfully prepared, a fascioliasis double-antibody sandwich ELISA detection method is constructed, and a theoretical basis is provided for research and development of a low-cost and rapid diagnostic kit.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to an anti-Faciella macula Cat L1 monoclonal antibody and its preparation method and application. Background technique [0002] Currently, Fasciola gigantica (Fg) is one of the pathogens of fascioliasis, which belongs to food-borne zoonotic parasites. The main hosts are dairy cows, buffaloes, goats and other livestock in tropical and subtropical regions. Worldwide, fascioliasis costs the livestock industry more than $3.2 billion annually. In addition, globally, at least 2.4 million people have been infected with fascioliasis, and at the same time, it threatens the health and safety of 180 million people. It is recognized by the World Health Organization as one of the most neglected tropical diseases. In my country, fascioliasis is mainly distributed in Guangxi, Yunnan and other places. From October to December 2011, 26 cases with persistent fever and liver injury ...

AI Technical Summary

Problems solved by technology

[0004] In recent years, the research on fasciola immunization and control has been continuously deepened, but the existing commercial diagnostic kits have the disadvantages of high cost and insufficient functions, and there is no commercial kit production in China

[0005] Through the above analysis, the problems and defects of the existing technology are: the existing commercial diagnostic kits have the disadvantages of high cost and insufficient functions, and there is no commercial kit production in China

[0006] The difficulty in solving the above problems and defects is as follows: the preparation process of commercial kits in my country still needs to be improved, and secondly, the workload of screening real and effective detection molecules is large and difficult

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