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Method for inducing differentiation of human adipose-derived stem cells co-cultured with umbilical cord mesenchymal stem cells into neural-like stem cells

A technology of adipose stem cells and mesenchymal stem cells, which is applied in the direction of cell culture active agents, biochemical equipment and methods, and culture processes, can solve the problems of low induction differentiation rate and poor stability of neural stem cells, etc., to avoid ethical issues, purity Consistent, safe results

Active Publication Date: 2021-09-28
东莞十度生物科技有限公司
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Problems solved by technology

[0011] However, at present, the differentiation rate of human-derived adipose-derived stem cells into neural stem cells is not high, and the stability of induced neural stem cells is poor

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  • Method for inducing differentiation of human adipose-derived stem cells co-cultured with umbilical cord mesenchymal stem cells into neural-like stem cells
  • Method for inducing differentiation of human adipose-derived stem cells co-cultured with umbilical cord mesenchymal stem cells into neural-like stem cells
  • Method for inducing differentiation of human adipose-derived stem cells co-cultured with umbilical cord mesenchymal stem cells into neural-like stem cells

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Embodiment Construction

[0034] The embodiments of the present invention are described in detail below, and the embodiments are only used to explain the present invention, and should not be construed as limiting the present invention.

[0035]The inventor, through creative research and development, uses co-cultivation of human adipose-derived stem cells and umbilical cord mesenchymal stem cells (UC-MSCs) and induces neural-like stem cells with cytokines. The induced differentiation rate is high, the purity is consistent, and the safety is high. This invention avoids ethical issues and the unsafety of allogeneic cell transplantation. The isolated and purified human adipose-derived adipose-derived stem cells can be transformed to obtain relatively consistent purity, non-gene-edited, safe and stable neural cells suitable for mass culture. Stem cells, suitable for individualized treatment of cerebral palsy, can provide a stable and feasible method for clinical treatment of cerebral palsy.

[0036] Co-cult...

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Abstract

The invention discloses a method for inducing differentiation of human adipose-derived stem cells into neural-like stem cells through co-cultured with umbilical cord mesenchymal stem cells and cell factors, and the neuron-like stem cells can be provided for medical institutions to treat nerve cell deficiency diseases such as child cerebral palsy and the like. According to the method, ethical problems and insecurity of allogeneic cell transplantation are avoided, the separated and purified human adipose-derived stem cells are transformed, neural stem cells which are consistent in purity, non-gene-edited, safe, stable and suitable for large-scale culture can be obtained, the neural stem cells are suitable for individualized treatment of the cerebral palsy, and a stable and feasible method can be provided for clinical treatment of the cerebral palsy.

Description

technical field [0001] The invention relates to a method for co-culturing human-derived adipose stem cells and umbilical cord mesenchymal stem cells to induce differentiation into neural-like stem cells, and belongs to the field of cell technology. Background technique [0002] Human adipose stem cells (ADSCs) are cells that exist in human adipose tissue and have multipotent differentiation ability. A large number of experiments have confirmed that in a certain induction environment, adipocytes can differentiate into bone cells, adipocytes, chondrocytes, and nerve cells. etc. After transplantation, it has good replacement, repair and therapeutic effects, and has no tumorigenicity, and does not cause immune rejection with the autologous body. [0003] Common methods for ADSCs to differentiate toward neural cells include culture in neural induction medium, co-culture, gene transfection, and electrical stimulation. [0004] Neural induction culture is induced by direct differe...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0797C12N5/0775
CPCC12N5/0623C12N2502/1382C12N2502/137C12N2501/999C12N2500/38C12N2501/11C12N2501/115C12N2501/13
Inventor 唐英王坤罗丽荣
Owner 东莞十度生物科技有限公司
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