Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for inducing differentiation of human adipose-derived stem cells co-cultured with umbilical cord mesenchymal stem cells into neural-like stem cells

A technology of adipose stem cells and mesenchymal stem cells, which is applied in the direction of cell culture active agents, biochemical equipment and methods, and culture processes, can solve the problems of low induction differentiation rate and poor stability of neural stem cells, etc., to avoid ethical issues, purity Consistent, safe results

Active Publication Date: 2021-09-28
东莞十度生物科技有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] However, at present, the differentiation rate of human-derived adipose-derived stem cells into neural stem cells is not high, and the stability of induced neural stem cells is poor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inducing differentiation of human adipose-derived stem cells co-cultured with umbilical cord mesenchymal stem cells into neural-like stem cells
  • Method for inducing differentiation of human adipose-derived stem cells co-cultured with umbilical cord mesenchymal stem cells into neural-like stem cells
  • Method for inducing differentiation of human adipose-derived stem cells co-cultured with umbilical cord mesenchymal stem cells into neural-like stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0034] The embodiments of the present invention are described in detail below, and the embodiments are only used to explain the present invention, and should not be construed as limiting the present invention.

[0035]The inventor, through creative research and development, uses co-cultivation of human adipose-derived stem cells and umbilical cord mesenchymal stem cells (UC-MSCs) and induces neural-like stem cells with cytokines. The induced differentiation rate is high, the purity is consistent, and the safety is high. This invention avoids ethical issues and the unsafety of allogeneic cell transplantation. The isolated and purified human adipose-derived adipose-derived stem cells can be transformed to obtain relatively consistent purity, non-gene-edited, safe and stable neural cells suitable for mass culture. Stem cells, suitable for individualized treatment of cerebral palsy, can provide a stable and feasible method for clinical treatment of cerebral palsy.

[0036] Co-cult...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
quality scoreaaaaaaaaaa
quality scoreaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for inducing differentiation of human adipose-derived stem cells into neural-like stem cells through co-cultured with umbilical cord mesenchymal stem cells and cell factors, and the neuron-like stem cells can be provided for medical institutions to treat nerve cell deficiency diseases such as child cerebral palsy and the like. According to the method, ethical problems and insecurity of allogeneic cell transplantation are avoided, the separated and purified human adipose-derived stem cells are transformed, neural stem cells which are consistent in purity, non-gene-edited, safe, stable and suitable for large-scale culture can be obtained, the neural stem cells are suitable for individualized treatment of the cerebral palsy, and a stable and feasible method can be provided for clinical treatment of the cerebral palsy.

Description

technical field [0001] The invention relates to a method for co-culturing human-derived adipose stem cells and umbilical cord mesenchymal stem cells to induce differentiation into neural-like stem cells, and belongs to the field of cell technology. Background technique [0002] Human adipose stem cells (ADSCs) are cells that exist in human adipose tissue and have multipotent differentiation ability. A large number of experiments have confirmed that in a certain induction environment, adipocytes can differentiate into bone cells, adipocytes, chondrocytes, and nerve cells. etc. After transplantation, it has good replacement, repair and therapeutic effects, and has no tumorigenicity, and does not cause immune rejection with the autologous body. [0003] Common methods for ADSCs to differentiate toward neural cells include culture in neural induction medium, co-culture, gene transfection, and electrical stimulation. [0004] Neural induction culture is induced by direct differe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0797C12N5/0775
CPCC12N5/0623C12N2502/1382C12N2502/137C12N2501/999C12N2500/38C12N2501/11C12N2501/115C12N2501/13
Inventor 唐英王坤罗丽荣
Owner 东莞十度生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com