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Kit for assisting in screening of familial papillary thyroid carcinoma

A kit, thyroid technology, applied in the field of kits for assisting in the screening of familial papillary thyroid carcinoma, achieving promising results

Active Publication Date: 2021-09-28
四川省三物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the relationship between CSTF3 gene mutation and thyroid cancer

Method used

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  • Kit for assisting in screening of familial papillary thyroid carcinoma
  • Kit for assisting in screening of familial papillary thyroid carcinoma
  • Kit for assisting in screening of familial papillary thyroid carcinoma

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0064] Experimental Example 1 Mutation Site Verification

[0065] 1. Subjects

[0066]Four patients were diagnosed with papillary thyroid carcinoma, and the disease status of their family members was as follows image 3 shown. Another 300 people who were diagnosed as having no papillary thyroid carcinoma were used as controls.

[0067] 2. Method

[0068] Take the peripheral blood of the aforementioned patients and their family members and 300 people as controls (the parents of the two families failed to obtain blood samples), extract DNA, and use the kit in the examples to detect the DNA containing base 33106691 of chromosome 11 Sequences were tested by Sanger sequencing.

[0069] 3. Results

[0070] There were 23 people in the two families, and 4 people had papillary thyroid carcinoma; the patients in the families all carried the chr1133106691 G>A mutation, while none of the unaffected people carried the chr11 33106691 G>A mutation, and none of the 300 controls carried c...

experiment example 2c

[0072] Experimental example 2 Chr11 33106691 G>A mutation carcinogenicity verification

[0073] 1. Subjects

[0074] Normal thyroid cell line (Nthy-ori 3-1).

[0075] Lentivirus expressing CSTF3 mutant gene (carrying chr11 33106691 G>A mutation corresponding site) (referred to as CSTF3 mutant lentivirus), expressing CSTF3 wild-type gene lentivirus (referred to as CSTF3 lentivirus), expressing GFP gene lentivirus (referred to as GFP lentivirus).

[0076] 2. Method

[0077] The CSTF3 mutant lentivirus was transfected into normal thyroid cell lines to verify the expression of cancer-related indicators (oncogenes: CDK4, CTSL2).

[0078] 3. Results

[0079] like image 3 As shown, the cells transfected with CSTF3 mutant lentivirus had significantly up-regulated expression of cancer index genes compared with cells transfected with CSTF3 lentivirus or GFP lentivirus.

[0080] The results showed that under the action of the CSTF3 mutant gene, normal thyroid cells transformed int...

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Abstract

The invention discloses a kit for assisting in screening of familial papillary thyroid carcinoma, and belongs to the field of gene polymorphism detection kits. The kit mainly comprises a reagent for detecting the variant human CSTF3 gene, and the gene is the CSTF3 gene corresponding to the 33106691th base of the eleventh chromosome of the genome changed from G to A. The invention further discloses an application of the reagent for detecting the base variation in preparation of a reagent for assisting in screening of familial papillary thyroid carcinoma and a kit for assisting in screening of the familial papillary thyroid carcinoma. The kit disclosed by the invention can be used for assisting in diagnosis of thyroid cancer and has an excellent application prospect.

Description

technical field [0001] The invention belongs to the field of gene polymorphism detection kits, in particular to a kit for assisting screening of familial papillary thyroid carcinoma. Background technique [0002] Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer, originating from follicular cells that produce and store thyroid hormones. The risk of PTC is closely related to gene polymorphisms, which explains the higher risk of PTC in those with a family history than those without. [0003] Several gene polymorphisms have been reported to be associated with the risk of PTC. For example, Wang Yingxue found that the 938C / A polymorphism of the Bcl-2 gene and the 9194A / G polymorphism of the Survivin gene were associated with the risk of PTC. PTC patients The C allele frequency of the Bcl-2 gene and the A allele frequency of the Survivin gene were higher than those in healthy controls (Wang Yingxue. Bcl-2, survivin gene polymorphisms and the risk of papi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 王朝红罗金强张俊彦钟强
Owner 四川省三物科技有限公司
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