Sudan black b2 dye and staining solution, detection method using same, gel electrophoresis system containing same, kit and preparation method

Abstract

The invention discloses a Sudan black B2 dye, a staining solution, a detection method using the same, a gel electrophoresis system containing the same, a kit and a preparation method. The Sudan black B2 dye is obtained by structural modification of Sudan black B. Through the technical scheme of the present invention, in the kit for determining lipoprotein subtyping and the gel electrophoresis system, the fat solubility of the dye can be greatly improved, so that the binding conditions of the dye and lipoprotein are simpler, the binding time is shorter, and the dyeing effect is improved. And the dyeing efficiency is higher; it can accurately distinguish each lipoprotein component, and at the same time qualitatively detect lipoprotein subtypes, including high-density lipoprotein, low-density lipoprotein and very low-density lipoprotein.

Description

Technical field [0001] The present invention relates to the field of biological detection, specifically a Sudanese black B2 dye and dyeing solution, using its detection method, containing its gel electrophoresis system, kit, preparation method and application. Background technique [0002] At present, for the detection of lipid metabolism, most kits are detected by single items, or use blood lipids simultaneously, but the content of low density lipoprotein is mainly calculated by formulas, often causes low density lipoprotein quantitative detection results. Inaccurate; for subtype detection, mainly used a nuclear magnetic resonance, non-denatured gradient gel electrophoresis, density gradient centrifugation, etc. . [0003] In addition, some of the gel electrophoresis methods detected lipoprotein sub-profiles, but due to the conventional Sudanese black b as diazo dyes, neutral fat can be blue, showing the best phospholipid effect; while each of the lipoproteins There is a differe...

AI Technical Summary

Problems solved by technology

[0002] At present, for the detection of lipid metabolism, most kits use a single item for detection, or use four blood lipid items for simultaneous detection, but the content of low-density lipoprotein is mainly calculated by the formula, which often leads to quantitative detection of low-density lipoprotein. Inaccurate; For the detection of subtypes, NMR, non-denaturing gradient gel electrophoresis, density gradient centrifugation and other methods are mainly used. These methods are time-consuming and laborious, and the test process is not easy to control. It requires professionals with high skills in large instruments to operate

[0003] In addition, some use gel electrophoresis to detect lipoprotein subtypes, but because the routinely used Sudan Black B is a diazo dye, it can make neutral fat appear blue-black, showing the best effect on phospholipids; There are differences in the main components of high-density and low-density lipoproteins, so the combination of Sudan Black B and each subtype of lipoprotein is not uniform, resulting in a large difference in staining results, resulting in the inability to qualitatively detect each subtype of lipoprotein at the same time. Types, including high-density lipoprotein, low-density lipoprotein, and very low-density lipoprotein

At the same time, since Sudan Black B needs to be dyed at 37°C, it is not easy to operate, which may easily lead to inaccurate experimental results.

For the above problems, there is no good solution

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