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Enterobacterludwigii for degrading ammonia nitrogen in white spirit wastewater and application of enterobacterludwigii

A technology of Enterobacter reuteri and white wine, which is applied to enterobacter reuteri for degrading ammonia nitrogen in liquor wastewater and its application field, can solve the problem of high nitrogen-containing compounds, achieve the effect of stable degradation ability and easy cultivation

Active Publication Date: 2021-10-15
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Anaerobic processes mainly include upflow anaerobic sludge bed (UASB), anaerobic biological filter (AF), anaerobic expanded granular sludge bed (EGSB), internal circulation anaerobic reactor (IC), etc. These processes It can effectively degrade most of the organic matter in liquor wastewater, but the nitrogen-containing compounds are still high, so further biological denitrification is required

Method used

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  • Enterobacterludwigii for degrading ammonia nitrogen in white spirit wastewater and application of enterobacterludwigii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Screening of Enterobacter ludwigii ZY-10, the steps are as follows:

[0042] (1) Take samples at the sewage treatment station of the liquor factory, select the activated sludge, and put it into a sterile sampling bag.

[0043](2) Weigh 2g of activated sludge and place it in a 250mL Erlenmeyer flask filled with 90mL of sterile saline, shake it well.

[0044] (3) Take the supernatant of the solution after mixing and dilute it successively to 10 times by 10 times dilution method. -2 、10 -3 、10 -4 ,

[0045] 10 -5 、10 -6 、10 -7 times the diluted solution.

[0046] (4) Use a sterile pipette gun to pipette 0.1mL of each dilution solution and apply it to 30mL of nitrifying bacteria enriched solid medium, and use a coating rod to evenly spread the bacterial solution on the surface of the medium; Large, the surface of the medium is not easy to dry after coating, and it is easy to cause bacterial contamination when inverted.

[0047] (5) Place in a constant temperature i...

Embodiment 2

[0057] The cultivation method of Enterobacter ludwigii ZY-10, the steps are as follows:

[0058] (1) Prepare 100 mL of seed liquid for cultivation in a 250 mL Erlenmeyer flask, and sterilize at 121°C for 20 minutes. The formula of the seed liquid medium is 4g of yeast extract, 8g of peptone, 9g of sodium chloride, and 1L of distilled water.

[0059] (2) Inoculate the Enterobacter ludwigii ZY-10 strain into the seed liquid medium on the aseptic operating table.

[0060] (3) Culture in a shaker at 37° C. and 150 r / min for 24 hours to obtain seed liquid.

[0061] (4) Prepare 100 mL of nitrifying bacteria enrichment liquid culture based on a 250 mL Erlenmeyer flask, and sterilize at 121° C. for 20 minutes. The formula of nitrifying bacteria enrichment liquid medium is 8g trisodium citrate, 1g dipotassium hydrogen phosphate, 2g ammonium sulfate, 0.4g magnesium sulfate heptahydrate, 0.6g sodium chloride, 0.5g ferrous sulfate heptahydrate, 1L distilled water, pH Adjust to 6.5.

...

Embodiment 3

[0069] Enterobacter ludwigii ZY-10 degrades ammonia nitrogen in liquor wastewater.

[0070] Proceed as follows:

[0071] (1) Take samples at the sewage treatment station of the liquor factory, take the liquor wastewater at the entrance of the secondary treatment pool, and put it into a sterile plastic bucket.

[0072] (2) Dilute the ammonia nitrogen in the liquor wastewater to 150 mg / L, when the pH is 4.3, take 100 mL and put it in a 250 mL Erlenmeyer flask, and sterilize at 121 °C for 20 min.

[0073] (3) On the aseptic operating table, insert 8% Enterobacter ludwigiiZY-10 activated bacterium liquid into the Erlenmeyer flask (the concentration of live bacteria is 7.0×10 5 CFU / mL).

[0074] (4) Culture in a shaker at 37°C and 150 r / min for 48 hours.

[0075] (5) In the blank group, the activated bacterial solution of Enterobacter ludwigii ZY-10 was replaced with sterile saline, and the other conditions were the same as those of the experimental group.

[0076] (6) After th...

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Abstract

The invention relates to the field of microorganisms, in particular to enterobacter ludwigii as well as a screening method and application thereof. The enterobacter ludwigii ZY-10 is screened from activated sludge of a wastewater treatment station of a liquor factory, and is preserved in the Guangdong Microbial Culture Collection Center on April 29, 2021, the preservation number is GDMCC No: 61633, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou City, Guangdong Province. The strain is easy to culture, and the ammonia nitrogen degradation rate is high. The strain is used for treating ammonia nitrogen in white spirit wastewater, and the degradation rate of the ammonia nitrogen reaches 60-85%. The invention provides an important strain for a nitration process in biological denitrification of the white spirit wastewater, lays a foundation for later construction of a compound strain for treating the white spirit wastewater, and has a relatively high application value.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation, and in particular relates to an Enterobacter reuteri strain degrading ammonia nitrogen in liquor wastewater and its application. Background technique [0002] With the rapid development of my country's liquor industry, the economic output has increased sharply, but a large amount of liquor wastewater will be produced in the production process. Liquor wastewater is mainly composed of distillation pot bottom water, flushing ancient wind pool water, kiln ground water, soaking discharge water, etc., which is a typical high-concentration organic wastewater. The main pollutants are starch, sugars, alcohols, lipids, cellulose, etc., so the treatment is difficult. If the liquor wastewater is discharged directly without treatment, it will inevitably cause eutrophication of the water body and the surrounding environment will also be polluted. [0003] At present, liquor wastewater generally ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F101/16C02F103/32C12R1/01
CPCC12N1/20C02F3/34C02F2101/16C02F2103/325Y02W10/10
Inventor 赵长青赵阳曾钰冉光雨吕枫方春玉
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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