Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A strain of Lactobacillus plantarum gbw-lp001 with high lactic acid production and its alternative antibacterial agent and application

A technology of Lactobacillus plantarum and antibacterial agent, which is applied in the field of microorganisms, can solve the problems of difficult stability of Lactobacillus plantarum and short storage time at room temperature, and achieve the effects of improving survival rate and slaughter weight, increasing growth performance, and improving breeding benefits

Active Publication Date: 2022-04-08
QINGDAO SHANGDE BIOTECH
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the stability of Lactobacillus plantarum has always been a problem, and its normal temperature storage time is short

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain of Lactobacillus plantarum gbw-lp001 with high lactic acid production and its alternative antibacterial agent and application
  • A strain of Lactobacillus plantarum gbw-lp001 with high lactic acid production and its alternative antibacterial agent and application
  • A strain of Lactobacillus plantarum gbw-lp001 with high lactic acid production and its alternative antibacterial agent and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Screening, isolation and molecular identification of Lactobacillus plantarum GBW-LP001

[0035] 1. Screening and purification of strains of Lactobacillus plantarum GBW-LP001

[0036] Different intestinal segments of healthy white-feathered broilers were sampled. The samples were isolated and purified on MRS liquid medium and MRS solid medium several times to obtain a strain of Lactobacillus with good acid production, and the single colony was named GBW-LP001.

[0037] Such as figure 1 As shown, the colony of the strain GBW-LP001 on the MRS plate is a very regular round, milky white, 2-3mm in diameter, smooth surface, neat edges, raised in the middle, opaque; under the microscope, the bacteria are short rod-shaped, Both ends are obtuse, single, paired or chain-like, without flagella, without spores.

[0038] 2. Identification of Lactobacillus plantarum GBW-LP001

[0039] (1) 16S rRNA gene identification: Using the DNA of the strain GBW-LP001 as a template, ...

Embodiment 2

[0045] Example 2: Stability and stress resistance analysis of Lactobacillus plantarum GBW-LP001

[0046] 1. Stability analysis of Lactobacillus plantarum GBW-LP001

[0047] The 100 billion CFU / g pure bacterial powder of Lactobacillus plantarum GBW-LP001 is obtained by fermenting in a fermenter, the fermentation temperature is 37°C, the fermentation time is about 12 hours, the carrier is skimmed milk powder, and the freeze-drying method is used. Market peer 1 is Lactobacillus plantarum produced by a company in Shanghai, with a bacterial volume of 200 billion CFU / g; market peer 2 is Lactobacillus plantarum produced by a company in Jiangsu, with a bacterial volume of 100 billion CFU / g; market peer 3 is a Taiwanese company The Lactobacillus plantarum produced by the company has a bacterial volume of 100 billion CFU / g; all three market samples can be purchased.

[0048] Seal different pure Lactobacillus plantarum powders, put them under the condition of 20+1°C, and track the numbe...

Embodiment 3

[0066] Example 3: Application case of Lactobacillus plantarum GBW-LP001 in breeding field

[0067] 1. Lactobacillus plantarum GBW-LP001 is used as feed for chicks in the breeder factory

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a strain of Lactobacillus plantarum GBW-LP001 with high lactic acid production and its alternative antibacterial agent and application. The classification of described plantarum lactobacillus GBW-LP001 is named plantaractobacillus Lactobacillus plantarum , the preservation number is CGMCC No.22078, the nucleotide sequence of its 16S rRNA is shown in SEQ ID No.1, and it has the effect of producing lactic acid greater than 18.1g / L after 24 hours of culture, and it is used as an opening for poultry animals The material has a good use effect. The present invention also utilizes Lactobacillus plantarum GBW‑LP001, Bacillus coagulans E21, Clostridium butyricum GBW‑N1, and glucose oxidase to jointly prepare a new alternative antibacterial agent, which can be used for the cultivation of poultry animals. Substituting antibiotics can achieve good breeding results and increase breeding benefits.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a strain of Lactobacillus plantarum GBW-LP001 with high lactic acid production, an alternative antibacterial agent and its application. Background technique [0002] As commercial feeds containing growth-promoting drug feed additives are gradually discontinued (referred to as "anti-antibiotics"). Probiotics, especially lactic acid bacteria, have become a new antibiotic replacement product, and their unique advantages have gradually been highlighted. [0003] Lactobacillus plantarum is a kind of Lactobacillus, which widely exists in human and animal intestines, silage fermented material, fermented food and dairy products. Usually Lactobacillus plantarum is discovered in the late stage of fermentation, mainly because Lactobacillus plantarum has high acid resistance and can tolerate a variety of adverse environments. At present, Lactobacillus plantarum has been used as a prob...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61K38/44A61K9/12A61P31/04A61K35/742C12R1/25
CPCC12N1/20A23K50/75A23K10/18A61K35/747A61K35/742A61K38/443C12Y101/03004A61K9/12A61K9/0078A61P31/04A23V2400/169A61K2300/00
Inventor 冯海霞吕伟王金龙赵斌师冬云
Owner QINGDAO SHANGDE BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com