Lactococcus lactis with depression improving effect, and application thereof

A technology of Lactococcus lactis and depression, which is applied in the field of microbiology, can solve problems such as weight gain, tremor and manic depression, and achieve good adhesion properties, improvement of depression efficacy, and excellent fermentation properties

Active Publication Date: 2021-10-19
HANGZHOU WAHAHA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of antidepressants has certain side effects, such as sweating, gastrointestinal discomfort, weight gain, insomnia, fatigue, nervousness, muscle tremors, and bipolar disorder

Method used

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  • Lactococcus lactis with depression improving effect, and application thereof
  • Lactococcus lactis with depression improving effect, and application thereof
  • Lactococcus lactis with depression improving effect, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Biological characteristics of Lactococcus lactis WHH2078

[0039] The bacterial strain Lactococcus lactis WHH2078 provided by the present invention is identified to belong to Lactococcus lactis (Lactobacillus selveticus), named as Lactococcus lactis, and was preserved on February 25, 2021 in the General Microorganism Center of China Microbiological Culture Collection Management Committee, Beijing, China No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, City, the microbial preservation number is CGMCC NO.21831.

[0040] The bacterial strain Lactococcus lactis WHH2078 provided by the present invention is isolated from the yoghurt ghee collected in the village of Shigatse City, Tibet Autonomous Region, my country.

[0041] The biological properties of bacterial strain Lactococcus lactis WHH2078 of the present invention are as follows:

[0042] Morphological characteristics: In the M17 agar medium, the growth form is a milky white colony, opaque, round, s...

Embodiment 2

[0048] The cultivation of embodiment 2 Lactococcus lactis WHH2078

[0049] After the second-generation activation of Lactococcus lactis WHH2078 of the present invention, it was inoculated in M17 medium with 1% inoculum size, cultivated at 37°C for 24 hours, and sampled once every 1 hour to measure its optical density (OD) at a wavelength of 600nm value, draw a growth curve, and set three replicates.

[0050] The result is as figure 2 As shown, Lactococcus lactis WHH2078, after the growth retardation period of 0-2h, begins to grow rapidly from 2h and enters the logarithmic growth phase, and ends the logarithmic phase at 6h and enters the stable phase.

Embodiment 3

[0051] The acid and bile salt resistance performance of embodiment 3 Lactococcus lactis WHH2078

[0052] After the second-generation activation of the bacterial strain Lactococcus lactis WHH2078 of the present invention and the commercial strain Lactobacillus rhamnosus GG (LGG), the bacterial liquid at the end of logarithmic growth was taken, centrifuged at 4000rpm for 10min, the supernatant was discarded, and the bacterial sludge was collected, and the following operations were performed respectively: ① Add the same volume of M17 culture solution with pH 2.5, 3.0 and 3.5, pipette and mix well, incubate at 37°C, and measure the number of bacteria at 0 point and after incubation for 3 hours by the dilution coating counting method; ②Add the same The volume of M17 culture solution containing 0.1%, 0.2% and 0.3% bile salts was pipetted and mixed, incubated at 37°C, and the number of bacteria after 0h and 8h incubation was measured by the dilution coating counting method. Strain su...

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Abstract

The invention relates to the technical field of microorganisms, and especially relates to a lactococcus lactis with the depression improving effect, and application thereof. The lactococcus lactis is named as Lactococcus lactis WHH2078, the strain is preserved in the China General Microbiological Culture Collection Center on February 25, 2021, and the preservation number of the strain is CGMCC NO.21831. The Lactococcus lactis WHH2078 disclosed by the invention has good adhesion characteristics, acid and cholate resistance and an intestinal barrier protection function, can be used for remarkably reducing the level of corticosterone in serum and relieving depression/anxiety-like behaviors by callback of in-vivo depression-related nerve factors, and has a remarkable depression improving effect; and the fermentation characteristic is excellent, and the strain can be applied to fermented milk and can also be applied to drugs, health care products or food for preventing and/or treating depression.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a strain of Lactococcus lactis with the effect of improving depression and its application. Background technique [0002] Depression is a common mental disorder. Patients feel depressed, lose interest or enjoyment, feel guilty, or have low self-esteem, sleep and appetite disturbances, fatigue, and difficulty concentrating. Depression can be long-term or recurring frequently, seriously affecting an individual's ability to work or school or to perform daily activities. At its worst, depression can lead to suicide. In 2020, depression has become the second leading cause of human death and disability after cancer. According to the report of the World Health Organization, there are about 350 million depression patients in the world, and there are more than 95 million depression patients in my country, and the number is increasing year by year. At the same time, the incidence of de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/744A61P25/24A61P25/22A23L33/00A23C9/12A23C9/152A23C19/00C12R1/46
CPCC12N1/20A61K35/744A61P25/24A61P25/22A23L33/00A23C9/1203A23C9/152A23C19/00A23V2002/00A23V2400/231A23V2200/30A23V2200/322
Inventor 高侃柯雪琴俞赟霞陈彩玲陈苏余腾斐李言郡汪海峰
Owner HANGZHOU WAHAHA TECH
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