Screening method of hypoglycemic drug

A technology for lowering blood sugar and drugs, which is applied in the establishment and application of drug screening methods. It can solve the problems of inability to evaluate the activity of active substances and mechanism of action, large differences between species in animal model experiments, and complex mechanism of action. The effect of high-throughput screening, high accuracy, and small sample volume

Pending Publication Date: 2021-10-26
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

However, due to the complex mechanism of islet cell chronic high-glucose injury, the animal model method has large differences among species, a long period, and poor reproducibility. Traditional chemical methods such as α-amylase and α-glucosidase or cytological methods The indicators are relatively single, and cannot comprehensively and accurately evaluate the activity and mechanism of active substances

Method used

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  • Screening method of hypoglycemic drug
  • Screening method of hypoglycemic drug
  • Screening method of hypoglycemic drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 builds a model

[0035] 1. Construction and verification of hyperglycemia cell model

[0036] Depend on figure 1 It can be seen that, compared with the control group (cells cultured with 5.6mM glucose), after 16.7mM and 33.3mM glucose acted on the cells for 24h, the cell survival rate decreased to varying degrees, and the cell survival rate of the 33.3mM glucose group decreased to (81.23 ± 1.21 )%. The 33.3mM glucose+5μmol / L metformin hydrochloride group was significantly higher than the 33.3mM glucose group (85.53±2.14)%, the difference was statistically significant (P<0.05). It shows that after 16.7mM and 33.3mM glucose acted on the cells for 24 hours, it has a damaging effect on INS-1 cells, while 33.3mM glucose + 5μmol / L metformin hydrochloride can alleviate the damage caused by high glucose on cells; it is finally determined that the 33.3mM treatment group is the model Group.

[0037] Pancreatic cells are specialized to sense elevated glucose level...

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Abstract

The invention provides a screening method of hypoglycemic drugs. Biomarkers related to hypoglycemia and content changes of the biomarkers are determined by analyzing model cell metabolites, so that a screening model of the hypoglycemic drugs is established and used for screening the hypoglycemic drugs, and a new mode is provided for screening of the drugs. According to the method disclosed by the invention, the antioxidant activity is comprehensively evaluated through a group or a group of biological targets through a system biological strategy, and compared with a single index, the method is more comprehensive and accurate. Cell metabonomics takes an endogenous substance of a living body as a research object, so that a complex life system is organically associated with antioxidant activity, and compared with a chemical method, the method is more representative.

Description

technical field [0001] The invention belongs to the technical field of establishment and application of drug screening methods, in particular to a screening method for hypoglycemic drugs. Background technique [0002] Diabetes mellitus is a disorder of glucose metabolism caused by the interaction of individual genetic factors and environmental factors (increased obesity rate, high-energy diet and low exercise level, etc.), eventually leading to pancreatic β-cell dysfunction. As of 2019, the number of people with diabetes worldwide has increased to 463 million, and it is estimated that 700 million people will suffer from diabetes by 2045. In China, nearly 90% of diabetic patients are type 2 diabetes mellitus (Type 2 Diabetes Mellitus, T2DM), whose dietary structure is restricted, the quality of life is reduced, and the medical burden is heavy. [0003] At present, the evaluation methods of hypoglycemic drugs mainly include chemical methods, cytological methods and animal mod...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/72G01N30/86G01N30/88G01N24/08
CPCG01N30/02G01N30/72G01N30/8696G01N30/8693G01N30/88G01N24/082G01N2030/8813G01N2030/8822G01N2560/00G01N2570/00
Inventor 邸多隆魏鉴腾裴栋
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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