Foot and mouth disease virus-like particulate antigen, and vaccine composition, preparation method and application thereof

The technology of foot-and-mouth disease virus and vaccine composition is applied in the field of viral antigens and pharmaceutical preparations of the antigens, and achieves the effects of good immunogenicity, high level of antibody production and long duration of immunity

Active Publication Date: 2021-10-29
PU LIKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented method involves making an effective way out against foot diseases caused by certain types of viruses called Footpad hepatitis B (FHB). By combining different protein structures with each other we creates particle sizes that are very small enough to stimulate strong humoral responses without causing harmful side reactions like those seen when administering some medications such as Fluconazole. These tiny particles have great potential because they may protect individuals from serious illness even if their symptoms last longer than 24 hours after exposure. They could potentially help stop new cases spread through these germs.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding suitable antigens for use against infective agents like Foot-And Mouth Disease Virus or other types of bacteria found naturally occurring throughout Africa such as cowpea mice. Current methods involve culturing these organisms at high cost, making them difficult to obtain without causing damage due to their small size.

Method used

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  • Foot and mouth disease virus-like particulate antigen, and vaccine composition, preparation method and application thereof
  • Foot and mouth disease virus-like particulate antigen, and vaccine composition, preparation method and application thereof
  • Foot and mouth disease virus-like particulate antigen, and vaccine composition, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Example 1 Type A foot-and-mouth disease virus-like particle

[0102] The bacterial cells expressing the antigenic protein of type A foot-and-mouth disease virus were resuspended, and detected by SDS-PAGE electrophoresis, which showed that three target proteins expressed in tandem in the supernatant were expressed at this time. The purified protein was detected by SDS-PAGE electrophoresis, which showed that the target protein was purified and enriched.

[0103] Negative staining with phosphotungstic acid and electron microscope observation showed that the type A foot-and-mouth disease protein had formed virus-like particles, and the formed virus-like particles were plump, with high assembly efficiency and no aggregation. After placing the foot-and-mouth disease virus-like particles at 4°C for 3 months, negative staining with phosphotungstic acid and electron microscope observation showed that the virus-like particles were still full without aggregation. It shows that th...

Embodiment 2

[0104] The preparation of embodiment 2 type A foot-and-mouth disease virus-like particle vaccine composition

[0105] The specific ratio of each component in the prepared vaccine is shown in Table 1.

[0106] Table 1 Composition ratio of type A foot-and-mouth disease virus-like particle vaccine composition

[0107] components Vaccine 1 Vaccine 2 Vaccine 3 FMD antigen (μg / ml) 160 200 240 Biphasic adjuvant (V / V%) 50％ 50％ 50％

Embodiment 3

[0108] Example 3 Immunogenicity Test of the Vaccine Composition Containing Type A Foot-and-Mouth Disease Virus-Like Particle Antigen

[0109] Select 20 healthy susceptible pigs with a body weight of about 40kg and are negative for type A foot-and-mouth disease virus antigen and antibody, and divide them into 4 groups randomly, 5 pigs in each group. Groups 1-3 are vaccine 1, vaccine 2, and vaccine 3 immunization groups prepared in Example 2 of the present invention, respectively, and group 4 is a blank control group. The way of immunization in the immunized group was intramuscular injection of 2 ml in the neck, and the control group was immunized with the same amount of PBS.

[0110] Antibody titer results showed that the antibody titer of all pigs before the vaccine immunization was negative, and all pigs could reach 1:128 or more on the 14th day after the first immunization. The antibody of pigs in the blank control group was negative and there was no change. The specific r...

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Abstract

The invention provides a type-A foot-and-mouth disease virus-like particulate antigen. The particulate antigen is formed by assembling antigen proteins VP2, VP3 and VP1 of type-A foot-and-mouth disease virus epidemic strains, and the antigen proteins VP2, VP3 and VP1 are separately coded by nucleotide sequences of specific sequences. A vaccine prepared from the type-A foot-and-mouth disease virus-like particulate antigen has good immunogenicity aiming at the type-A foot-and-mouth disease virus epidemic strains. Vaccines prepared from the antigen and a type-O foot-and-mouth disease virus-like particulate antigen can respectively protect the type-A foot-and-mouth disease virus epidemic strains and type-O foot-and-mouth disease viruses.

Description

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Claims

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Application Information

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Owner PU LIKE BIO ENG
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