Colloidal gold test strip for detecting human calcitonin

A test strip and calcitonin technology, which is applied in the field of biomedical detection, can solve the problems of complex operation process, high operator requirements, slow detection speed, etc., and achieves the effects of wide application range, convenient and fast use, and stable markers.

Inactive Publication Date: 2021-11-02
施康培医药科技(武汉)有限公司
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  • Abstract
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Problems solved by technology

[0006] Traditional calcitonin detection methods include immunoradiometric assay (IRMA) and electrochemiluminescence. These methods have complex operation procedures, high requirements for operators, are suitable for high-throughput samples, and the detection speed is slow (1 hour-1 day)

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  • Colloidal gold test strip for detecting human calcitonin
  • Colloidal gold test strip for detecting human calcitonin

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Embodiment Construction

[0021] The principles and features of the present invention are described below, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0022] 1. Preparation of Human Calcitonin Epitope Peptide

[0023] The amino acid sequence of human calcitonin was analyzed to predict its epitope peptide region, and two antigenic epitope peptides were obtained, the amino acid sequences of which are shown in SEQ ID NO: 1 and 4, respectively.

[0024] The epitope peptides SEQ ID NO:2 and 3 were redesigned according to the peptide SEQ ID NO:1, and the epitope peptides SEQ ID NO:5 and 6 were redesigned according to the peptide SEQ ID NO:4.

[0025] The peptides of SEQ ID NO: 1-3 were synthesized respectively, linked to the carrier protein BSA, and mixed to prepare the first antigen. Peptides of SEQ ID NO:4-6 were synthesized respectively, linked to the carrier protein BSA, and mixed to prepare the second antigen...

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Abstract

The invention relates to a test strip for detecting human calcitonin, which comprises a bottom plate, and a sample pad, a colloidal gold combination pad, an analysis membrane and a sample absorption pad which are arranged on the bottom plate and connected in sequence, and a detection line is arranged on the analysis membrane; the colloidal gold combination pad contains colloidal gold particles labeled by a combined antibody; and a capture antibody is anchored in the detection line, both the binding antibody and the capture antibody can specifically recognize and bind with the calcitonin, and binding sites of the binding antibody and the capture antibody with the calcitonin do not interfere with each other. The test strip disclosed by the invention can be used for quantitatively analyzing the calcitonin in serum, whole blood and punctured tissue eluent, is convenient and rapid to use, is convenient to use on a base layer and on site, can complete all reactions within 15 minutes, is wide in application range, is stable in marker, and can be stored for more than two years at 4 DEG C; colloidal gold is red and is non-toxic to a human body.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and in particular relates to a colloidal gold test strip for detecting human calcitonin. Background technique [0002] Medullary thyroid carcinoma (MTC) originates from the canceration of thyroid parafollicular cells (also known as C cells), which belong to neuroendocrine cells. Its main feature is the secretion of calcitonin and various substances including carcinoembryonic antigen, and the production of amyloid. [0003] Calcitonin is a peptide hormone secreted by the parafollicular C cells of the thyroid gland. Its precursor preprocalcitonin has 136 amino acids, and the mature calcitonin has 32 amino acids. There is a disulfide bond between its 1st and 7th amino acids, which, like the C-terminal proline amide, is crucial to the biological function of calcitonin. The synthesis and secretion of calcitonin are strictly regulated, and the concentration of calcitonin is almost linear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/577G01N33/558G01N33/543G01N33/58
CPCG01N33/57407G01N33/57488G01N33/577G01N33/558G01N33/54346G01N33/587G01N2333/585
Inventor 王珏
Owner 施康培医药科技(武汉)有限公司
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