Chiral fluorescence confocal imaging system and imaging method

Abstract

The invention relates to the technical field of optics, and particularly discloses a chiral fluorescence confocal imaging system and method. The chiral fluorescence confocal imaging system comprises a laser device, an excitation light path, a microscope, a sample table, a detection light path, a photoelectric detector and a control device, wherein laser emitted by the laser device is modulated by the excitation light path to generate polarized light, the polarized light irradiates a to-be-detected sample on the sample table through the microscope and excites the to-be-detected sample to generate a fluorescence signal, the fluorescence signal is modulated by the detection light path and transmitted to the photoelectric detector, the photoelectric detector converts the fluorescence signal into an electric signal and forms an image, and the control device is connected with the photoelectric detector and the sample table. According to the chiral fluorescence confocal imaging system, the imaging resolution of a traditional optical microscope can be improved, and laser confocal imaging is achieved.

Description

technical field [0001] The invention relates to the field of optical technology, and specifically discloses a chiral fluorescence confocal imaging system and an imaging method. Background technique [0002] Laser scanning confocal microscope (Laser scanning confocal microscope) is a new technology developed in the mid-1980s and widely used. The advanced cell and molecular biology analysis instruments produced by the combination are more and more widely used in the fields of biology and medicine, and have become an essential tool for biomedical experimental research. [0003] Traditional fluorescence microscopes use fluorescent substances to mark specific structures in cells, which not only enhances the contrast between the image and the background, but also greatly improves the resolution (δ=0.61·λ / NA, where δ is the resolution of the microscope; λ is the wavelength of the illumination light; NA is the numerical aperture of the objective lens). However, when the observed f...

AI Technical Summary

Problems solved by technology

However, in the field of microscopy imaging, there is a lack of imaging instruments for the study of chiral fluorescence

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