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Application of c-Src/PI3K signal channel in reproductive injury protection

A signaling pathway, 1.c-src technology, applied in the fields of biotechnology and medicine, can solve problems such as unclear intervention effect, and achieve the effect of improving cell connection damage and spermatogenesis microenvironment

Pending Publication Date: 2021-11-16
BEIHUA UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the intervention effect of ginsenosides on reproductive damage induced by environmental toxicants is unclear

Method used

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  • Application of c-Src/PI3K signal channel in reproductive injury protection
  • Application of c-Src/PI3K signal channel in reproductive injury protection
  • Application of c-Src/PI3K signal channel in reproductive injury protection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Mouse validation experiments.

[0057] 1. Preparation and grouping of animal models.

[0058] Thirty 6-week-old SPF grade male C57BL / 6J mice, weighing 20±2g. Experimental animals were divided into control group, DBP group (400mg / kg) and Rg3+DBP group (400mg / kgDBP+20mg / kgRg3). Administration method The animals in DBP group and DBP+Rg3 group were orally administered 400 mg / kg DBP (dissolved in corn oil) at 8:00 every day. Rg3 group and DBP+Rg3 group received intraperitoneal injection of Rg320mg / kg (dissolved in normal saline) at 2 p.m. on the same day, and the control group was given intragastric administration of equal volume of corn oil at 8 a.m. every day, and intraperitoneal injection of normal saline at 2 p.m. on the same day. All experiments Animals were administered drugs for 35 days in a row. After the experiment period was over, ether anesthetized, 10 mL of blood was collected from the abdominal aorta, and after standing at room temperature, 2500 r min -1 Aft...

Embodiment 2

[0094] T4 cell verification experiment.

[0095] 1. Cell culture and grouping.

[0096] In order to confirm the role of c-Src / PI3K signaling pathway in the damage of DBP to spermatogenesis and the protection of ginsenoside Rg3, the effects of PI3K-specific inhibitor LY294002 and c-Src inhibitor PP2 on T4 cells were added to the in vitro experimental design. In addition, because DBP is mainly absorbed into the body in the form of its metabolites, and the toxicity and activity of DBP after metabolizing MBP in the body will be higher than DBP, so a series of experiments were carried out with MBP in cell experiments. The cells were divided into 8 groups: DMSO group, MBP group, Rg3 group, MBP+Rg3 group, MBP+LY294002 group, MBP+Rg3+LY294002 group, MBP+PP2 group, MBP+Rg3+PP2 group. The cells of each group were conventionally cultured in DMEM / F12 medium containing 10% (volume fraction) fetal bovine serum, and the culture environment was 37°C, 95% (volume fraction) air, 5% (volume fra...

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Abstract

The invention relates to application of a c-Src / PI3K signal channel in reproductive injury protection, and relates to the technical field of biotechnology and medicine. The invention provides an application of a c-Src / PI3K signal channel as a target spot in preparation of drugs for treating reproductive injury, and provides a new action target spot for developing natural spermatogenic drugs.

Description

technical field [0001] The invention relates to the fields of biotechnology and medical technology, in particular to the application of a c-Src / PI3K signaling pathway in the protection of reproductive damage. Background technique [0002] In recent years, the fertility rate of couples of childbearing age has gradually declined. According to data, about 15% of couples in the world are affected by infertility, and 20% to 30% of the cases are caused by male infertility and the couple's infertility. Traditional Chinese medicine has unique advantages and good curative effects in the treatment of male infertility and spermatogenesis disorders. In the drug treatment of infertility, ginseng, known as the "king of Chinese herbal medicine", is the drug of choice for the treatment of infertility. It has been reported in the literature that ginseng extract (GINST) treated with pectinase has antioxidant effect and can effectively treat testicular damage in rats caused by heat stress, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/704A61P15/00A61P15/08
CPCA61K45/00A61K31/704A61P15/00A61P15/08
Inventor 李环张晶徐小磊王洪艳
Owner BEIHUA UNIV
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