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Mature plant rhizosphere soil microdomain research device and method

A plant root and soil technology, applied in the research device field of mature plant rhizosphere soil micro-domain

Pending Publication Date: 2021-11-16
ZHEJIANG UNIV HANGZHOU GLOBAL SCI & TECH INNOVATION CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention intends to provide a research device and method for the rhizosphere soil micro-domain of mature plants, so as to solve the problem that the microfluidic chip is not used in the prior art to study the rhizosphere of mature plants

Method used

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  • Mature plant rhizosphere soil microdomain research device and method
  • Mature plant rhizosphere soil microdomain research device and method
  • Mature plant rhizosphere soil microdomain research device and method

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Embodiment Construction

[0023] Below in conjunction with accompanying drawing and embodiment the present invention is described in further detail:

[0024] The reference signs in the drawings of the description include: root box 1, microfluidic chip 2, substrate 21, cover sheet 22, inner cavity 3, liquid inlet hole 4, liquid outlet hole 5, plant root inlet 6, liquid inlet channel 7. Pipeline 8, micro column 9, plant channel 10.

[0025] like figure 1 , figure 2 and image 3 As shown, a research device of a mature plant rhizosphere soil micro-domain, including a microfluidic chip 2 for cultivating and observing plant roots and a root box 1 for planting mature plant plants, the microfluidic chip 2 includes a base sheet 21 and cover sheet 22, the inner surface of the substrate 21 is engraved with a microchannel, and the microchannel and the inner surface of the cover sheet 22 are enclosed to form the inner cavity 3 of the microfluidic chip 2, and the microfluidic chip 2 is also provided with a liqui...

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Abstract

The invention discloses a mature plant rhizosphere soil micro-domain research device. The device comprises a micro-fluidic chip and a root box; the micro-fluidic chip comprises a substrate and a cover plate; a micro-channel is engraved on the inner surface of the substrate; an inner cavity of the micro-fluidic chip is defined by the micro-channel and the inner surface of the cover plate; the micro-fluidic chip is provided with a liquid inlet hole, a liquid outlet hole and a plant root inlet, the liquid inlet hole, the liquid outlet hole and the plant root inlet are all communicated with the inner cavity; a through hole is formed in the bottom of one side wall of the root box; the root box is connected with a pipeline through the through hole; a connector is arranged at the free end of the pipeline; the pipeline is communicated with the plant root inlet through the connector; and the pipeline and the connector are both filled with cotton and quartz sand. According to the mature plant rhizosphere soil microdomain research device and method of the invention, a complete system for plant rhizosphere micro-domain research is constructed, chemical environment in-situ dynamic research and microorganism in-situ dynamic research are realized, and further exploration of soil-root system microorganism interaction is facilitated.

Description

technical field [0001] The invention relates to the technical field of plant rhizosphere micro-domains, in particular to a research device and method for mature plant rhizosphere soil micro-domains. Background technique [0002] The rhizosphere generally refers to a specific micro-ecological system formed by plants, soil and various environmental factors within a few millimeters from the root surface. It gathers a large number of bacteria, fungi and other microorganisms as well as earthworms and nematodes around the root system of plants. Soil fauna, forming a special biome, is the most active area of ​​soil-root microbe interactions. The soil is an opaque medium, and the respective mechanisms in the soil are more of a "black box" for us. There have always been obstacles in the study of the rhizosphere soil micro-domain, which needs to be uncovered by emerging technologies. Root boxes are traditionally used, such as three-chamber multi-compartment root boxes for millimeter-...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/30B01L3/00
CPCG01N21/6458G01N1/30B01L3/5027B01L2300/0861B01L2300/0887B01L2200/10
Inventor 戴恒毅马斌吴禹徐建明
Owner ZHEJIANG UNIV HANGZHOU GLOBAL SCI & TECH INNOVATION CENT
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