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Method for researching rhizosphere micro-region and used micro-fluidic chip device

A microfluidic chip and micro-domain technology, applied in the biological field, can solve problems such as ignoring the physical structure of soil, and achieve the effect of solving research obstacles

Active Publication Date: 2019-09-06
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The current microfluidic chip uses a chip with an independent cavity to study the microdomain of the plant rhizosphere, which ignores the influence of soil physical structure on the rhizosphere and microorganisms; and also simulates the microfluidic structure of the soil to a certain extent. control chip, few choose to add plant factors to explore the micro-domain of the rhizosphere

Method used

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  • Method for researching rhizosphere micro-region and used micro-fluidic chip device
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  • Method for researching rhizosphere micro-region and used micro-fluidic chip device

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Embodiment 1

[0037] Embodiment 1. A microfluidic chip device for studying rice rhizosphere micro-domains, including a glass slide 1 and a microfluidic chip 2, the glass slide 1 is a standard glass slide, and the microfluidic chip 2 is made of PDMS It is formed by pouring; the microfluidic chip 2 is located on the upper surface of the slide glass 1 .

[0038] The bottom of the microfluidic chip 2 is provided with a groove to form a cavity 202, so that the microfluidic chip 2 naturally forms an upper chip 201 and a lower chip, and the slide glass 1 is close to the cavity 202, so that the cavity 202 forms a relatively sealed cavity. That is, the microfluidic chip 2 of PDMS pouring can be regarded as being made up of upper and lower two parts; 202) composition (the upper part and the lower part are actually a whole).

[0039] The length of the entire microfluidic chip 2 is about 70mm, the broadband is about 20mm, and the thickness is about 1cm. The height of the cavity 202 is about 50μm, and...

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Abstract

The invention discloses a micro-fluidic chip device for researching a rice rhizosphere micro-region. The micro-fluidic chip device comprises a glass slide (1) and a micro-fluidic chip (2), a cavity (202) is formed in the bottom of the micro-fluidic chip (2), so that the micro-fluidic chip (2) is divided into an upper chip (201) and a lower chip, the glass slide (1) is closely fitted with the cavity (202), a left micro-column group (203) and a right micro-column group (204) are arranged in the cavity (202), a plant channel (22) is naturally formed in a gap between the left micro-column group (203) and the right micro-column group (204), root systems grow in the plant channel (22), a liquid inlet hole (23), a liquid outlet hole (24) and a plant hole (25) communicated with the cavity (202) are formed in the upper chip (201), and an outlet of the plant hole (25) corresponds to the plant channel (22). The invention further discloses a method for researching the rhizosphere micro-region by the aid of the micro-fluidic chip device.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a chip and a corresponding method for studying rhizosphere micro-domains by means of microfluidic technology. Background technique [0002] The rhizosphere generally refers to a specific micro-ecological system formed by plants, soil and environmental conditions within a few millimeters from the surface of the root shaft, and it is also the area where soil root-microbe interactions are most active. [0003] Studying rhizosphere microdomains has been hindered due to the opaque nature of soil. Root boxes are traditionally used, such as three-chamber multi-compartment root boxes for millimeter-scale sampling, but the impact of this destructive sampling on the microbial community structure is difficult to ignore. [0004] The microfluidic chip was proposed by A.Manz in the early 1990s. It is an important science and technology that continues to develop. In 2006, the Nature magazin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/02
CPCA01G31/02Y02P60/21
Inventor 叶舒迪林楠马斌吴禹徐建明
Owner ZHEJIANG UNIV
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