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Method for studying rhizosphere microdomains and microfluidic chip device therefor

A microfluidic chip and microdomain technology, which can be used in tissue cell/virus culture devices, botanical equipment and methods, biochemical equipment and methods, etc. Effect

Active Publication Date: 2021-02-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The current microfluidic chip uses a chip with an independent cavity to study the microdomain of the plant rhizosphere, which ignores the influence of soil physical structure on the rhizosphere and microorganisms; and also simulates the microfluidic structure of the soil to a certain extent. control chip, few choose to add plant factors to explore the micro-domain of the rhizosphere

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  • Method for studying rhizosphere microdomains and microfluidic chip device therefor
  • Method for studying rhizosphere microdomains and microfluidic chip device therefor
  • Method for studying rhizosphere microdomains and microfluidic chip device therefor

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Embodiment 1

[0037] Embodiment 1. A microfluidic chip device for studying rice rhizosphere micro-domains, including a glass slide 1 and a microfluidic chip 2, the glass slide 1 is a standard glass slide, and the microfluidic chip 2 is made of PDMS It is formed by pouring; the microfluidic chip 2 is located on the upper surface of the slide glass 1 .

[0038] The bottom of the microfluidic chip 2 is provided with a groove to form a cavity 202, so that the microfluidic chip 2 naturally forms an upper chip 201 and a lower chip, and the slide glass 1 is close to the cavity 202, so that the cavity 202 forms a relatively sealed cavity. That is, the microfluidic chip 2 of PDMS pouring can be regarded as being made up of upper and lower two parts; 202) composition (the upper part and the lower part are actually a whole).

[0039] The length of the entire microfluidic chip 2 is about 70mm, the broadband is about 20mm, and the thickness is about 1cm. The height of the cavity 202 is about 50μm, and...

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Abstract

The invention discloses a microfluidic chip device for studying rice rhizosphere microdomains, which comprises a glass slide (1) and a microfluidic chip (2). cavity (202), so that the microfluidic chip (2) is formed on the chip (201) and under the chip, and the slide glass (1) is close to the cavity (202); in the cavity (202), the left micro Column group (203) and right microcolumn group (204); The space between left microcolumn group (203) and right microcolumn group (204) forms the plant channel (22) for root system growth naturally; On chip (201 ) are respectively provided with a liquid inlet hole (23), a liquid outlet hole (24), and a plant hole (25) communicated with the cavity (202); the outlet of the plant hole (25) corresponds to the plant channel (22). The invention also discloses a method for researching the rhizosphere micro-domain by using the microfluidic chip device at the same time.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a chip and a corresponding method for studying rhizosphere micro-domains by means of microfluidic technology. Background technique [0002] The rhizosphere generally refers to a specific micro-ecological system formed by plants, soil and environmental conditions within a few millimeters from the surface of the root shaft, and it is also the area where soil root-microbe interactions are most active. [0003] Studying rhizosphere microdomains has been hindered due to the opaque nature of soil. Root boxes are traditionally used, such as three-chamber multi-compartment root boxes for millimeter-scale sampling, but the impact of this destructive sampling on the microbial community structure is difficult to ignore. [0004] The microfluidic chip was proposed by A.Manz in the early 1990s. It is an important science and technology that continues to develop. In 2006, the Nature magazin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00
CPCA01G31/02Y02P60/21
Inventor 叶舒迪林楠马斌吴禹徐建明
Owner ZHEJIANG UNIV
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