Equipment and method for stimulating secretion of cell exosomes, obtained exosomes and application of exosomes

Abstract

The invention provides equipment and a method for stimulating secretion of cell exosomes, the obtained exosomes and application of the exosomes. The equipment for stimulating secretion of the cell exosomes outputs an electric signal to an interdigital transducer through a signal generator to generate a surface acoustic wave signal, the surface acoustic wave signal stimulates cells in a storage device, the cells are subjected to the mechanical effect and the heat effect of the surface acoustic wave by utilizing the characteristic of large acoustic impedance of the storage device, cell perforation is caused, and the secretion number of the cell exosomes is increased. The survival rate of the cells secreted by the cell exosomes stimulated by the equipment is high, the secretion speed is high, and the obtained exosomes are high in effectiveness and good in treatment effect.

Description

technical field [0001] The present invention relates to the technical field of medical devices, in particular to a device and method for stimulating exosome secretion from cells, the obtained exosome and its application. Background technique [0002] In the human body, cellular exosomes can transmit information between cells through the circulatory system. The lipid bilayer membrane structure of exosomes can not only protect internal proteins and nucleic acids from degradation, but also maintain the intrinsic target of their parent cells. tropism, which makes them potentially useful as efficient carriers for delivering therapeutic drugs into recipient cells. The CD47 protein on exosomes also prevents them from being phagocytized by immune cells, making exosomes more effective than synthetic lipoids. A large number of studies have shown that RNA in exosomes can affect the functional characteristics of cells that absorb it during the process of intercellular communication, wh...

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Problems solved by technology

[0020] (1) Although the addition of ionomycin and calcium phosphate can increase the content of exosomes, excessive exposure of cells to these chemicals will lead to a significant decrease in their viability

[0021] (2) Exposure of cells to ionizing radiation can also lead to apoptosis

[0022] (3) On the other hand, it has been reported that oxidative stress can increase exosome production by about 20-30 times within 24 hours (about 0.8-1.25 times / hour), but can produce immunoreactive exosomes, which May impair its diagnostic or therapeutic potential

[0023] (4) Mechanical means such as cyclic stretching can lead to damage to the integrity of the cell membrane, which will affect its therapeutic activity and pose a safety risk

[0024] (5) At present, the only human cell type known to have the ability to produce exosomes on a large scale is mesenchymal stem cells (MSCs). Although MSCs have a huge ability to expand in vitro, new MSCs need to be continuously extracted to Complementing the cell source of exosomes, each cell source requires repeated extraction, testing and verification costs, making the production of MSC exosomes commercially unavailable

[0025] (6) Electroporation will lead to the aggregation of exosomes or the charged drugs wrapped in them, thus affecting the therapeutic efficacy of exosomes

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