Preparation and application of guided bone tissue regeneration membrane containing resveratrol liposome

Abstract

The invention discloses preparation and application of a guided bone tissue regeneration membrane containing resveratrol liposome, and belongs to the field of medical materials. The preparation comprises the following steps of: 1, dissolving a medical degradable material in an organic solvent to obtain an electrospinning matrix solution; 2, preparing resveratrol into a resveratrol liposome solution by adopting an ethanol injection method in advance, obtaining resveratrol liposome by a freeze-drying method, and dissolving the resveratrol liposome in the electrospinning matrix solution to prepare an electrospinning solution; 3, performing electrospinning on the prepared electrospinning solution by adopting an electrospinning technology to obtain an electrospinning membrane; and 4, drying the electrospinning membrane to obtain a functional bone tissue regeneration membrane. The guided bone tissue regeneration membrane containing the resveratrol liposome, prepared by the preparation, has high biocompatibility and controllable degradation performance; and an excellent three-dimensional scaffold structure can effectively load and locally and slowly release active matter resveratrol, so that the aims of promoting migration and proliferation of osteoblasts, promoting osteogenic differentiation and improving osteogenic performance are fulfilled.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and relates to a degradable and resveratrol-modified guiding bone tissue regeneration film containing active substances, a preparation method and an application. Background technique [0002] Guided Bone Regeneration (English full name: Guided Bone Regeneration, referred to as GBR) technology is one of the most commonly used and most effective technologies to solve the problem of bone tissue defect in clinical practice. It is to place the guided bone tissue regeneration barrier membrane between soft tissue and bone defect Artificially establish a biological barrier and create an isolation space to prevent the growth of fibroblasts and epithelial cells in the soft tissue into the bone defect area, ensure that there is no interference of fibroblasts in the osteogenesis process, and finally achieve complete bone repair in the defect area; this technology is used in the oral cavity The peri...

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Problems solved by technology

[0004] In addition to choosing a suitable GBR membrane preparation method, the selection of its preparation materials is equally important; although GBR membranes prepared from medically degradable materials can be used for further clinical applications of bone implant materials and the realization of guided bone tissue regeneration membranes to a certain extent. However, it still has great shortcomings in guiding bone tissue regeneration, which limits the clinical application of electrospun membranes to a certain extent; and, in clinical studies, it was found that large areas of bone tissue Damage is generally accompanied by the threat of inflammation, which hinders further repair of bone tissue; studies have shown that resveratrol can be used in a wide range of applications, including the treatment of osteoporosis and the enhancement of bone formation, fracture repair or bone reconstruction defects, resveratrol Alcohol can promote the differentiation of bone marrow stromal cells into osteoblasts, and increase the activity of alkaline phosphatase and prolyl hydroxylase in a dose-dependent manner; It is easily oxidized under the action of oxidants and has poor water solubility. It has the disadvantages of poor oral absorption, low bioavailability, and long-lasting effect. This aspect limits the use of resveratrol in some fields to a large extent Therefore, by making resveratrol into nano-liposomes for research; it can effectively protect the encapsulated drug to prevent the functionality of the drug and some components from being oxidized, thereby improving the stability and biological utilization of the drug It can also be administered through a variety of routes, so that it has the characteristics of targeting, sustained release and cell affinity.

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