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Recombinant protein for eliminating boar taint and vaccine composition comprising same

A vaccine composition and recombinant protein technology are applied in the field of vaccine compositions for eliminating male odor and can solve the problem of high cost and expenditure

Pending Publication Date: 2021-12-03
BIOAPPL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] But, as far as the domestic situation is concerned, it is not 100% to meet the castration judgment standard only based on adopting the immune castration method to eliminate male odor or improve the meat quality. Compared with the surgical castration method, its expense expenditure is very high (if the surgical castration method needs 100 per head KRW, and 5,000 KRW per head for immune castration)

Method used

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  • Recombinant protein for eliminating boar taint and vaccine composition comprising same
  • Recombinant protein for eliminating boar taint and vaccine composition comprising same
  • Recombinant protein for eliminating boar taint and vaccine composition comprising same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Embodiment 1: Expression and refining of BiP-CTB-GnRH-pFc2-HDEL fusion protein

[0097] To induce the immunogenicity of GnRH, as figure 1 As shown, a vector for expressing recombinant GnRH in which CTB, pFc2, BiP and HDEL were fused to GnRH was produced. Six GnRHs consisting of 10 amino acids are connected consecutively by alanine-glycine-alanine (AGA) linkers, and the amino acid sequences and base sequences of the six connected GnRHs are shown in SEQ ID NO: 2 and SEQ ID NO: 3, respectively. . In addition, the amino acid sequence and base sequence of CTB are shown in SEQ ID NO: 4 and SEQ ID NO: 5, the amino acid sequence and base sequence of pFc2 are shown in SEQ ID NO: 6 and SEQ ID NO: 7, respectively, and the amino acid sequence and base sequence of BiP They are shown in SEQ ID NO: 8 and SEQ ID NO: 9, respectively, and the amino acid sequence and base sequence of HDEL are shown in SEQ ID NO: 10 and SEQ ID NO: 11, respectively. Meanwhile, the entire amino acid seq...

Embodiment 2

[0099] Embodiment 2: confirmation of recombinant GnRH protein concentration

[0100] In order to confirm and measure the concentration of the recombinant GnRH protein isolated and refined in Example 1, bovine serum albumin (BSA, bovine serum albumin) of known concentration was used as a standard (standard), and SDS-PAGE and Brasil were carried out. Deford quantitative analysis (bradford assay). After loading 1 and 2 μl of the isolated and refined recombinant GnRH protein, 0.5, 1 and 2 μg of BSA were contained therein for quantitative processing. Gel finally confirmed the protein band by Coomassie blue staining (Coomassie bluestaining). Bradford Quantitative Analysis used BSA as a standard to draw a standard curve (standard curve) and formulate it, and then substituted the UV value of GnRH to calculate the concentration.

Embodiment 3

[0102] Embodiment 3: the injection effect confirmation of recombinant GnRH protein

[0103] 3.1. Experimental animals, injection conditions and schedule

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Abstract

The present invention relates to a vaccine composition for eliminating boar taint, comprising a recombinant protein in which a cholera toxin B subunit (CTB) and a gonadotropin-releasing hormone (GnRH) are fused. More specifically, the present invention provides: a recombinant protein for eliminating boar taint, for inducing an antibody against GnRH; a recombinant vector for producing same; a vaccine composition for eliminating boar taint, comprising the recombinant protein; and a method for eliminating boar taint using the vaccine composition. The vaccine composition according to the present invention has an effect of inducing an antibody against GnRH in a subject, thereby atrophying the testis. Therefore, the composition can be effectively used to eliminate boar taint by immunologically castrating boars at a low cost and with high safety and minimal side effects.

Description

technical field [0001] The invention relates to a vaccine composition for eliminating male odor. Specifically, it relates to a recombinant protein for eliminating male odor for inducing gonadotropin-releasing hormone (GnRH) antibodies, a recombinant vector for producing the recombinant protein, and a recombinant protein containing the recombinant protein as an active ingredient. A vaccine composition for eliminating male odor and a method for eliminating male odor using the vaccine composition. [0002] This application claims priority based on Korean Patent Application No. 10-2020-0035897 filed on March 24, 2020, and all the contents described in the specification and drawings of the related application are incorporated herein by reference. Background technique [0003] "Boar taint (boar taint)" refers to an unpleasant smell or taste that is often produced during cooking or eating pork or pork products, although it is not at all problematic for the safety of meat to eat, b...

Claims

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Application Information

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IPC IPC(8): A61K39/385A61K39/00A61P5/02C07K19/00C12N15/63
CPCA61K39/385A61K39/0005A61P5/02C07K7/23C12N15/63C07K2319/55C07K2319/30C07K2319/35A61P15/00C07K14/28A61P5/24A61K39/0006A61K2039/6037A61K2039/552C07K14/70503C07K7/06A61K2039/6031A61K2039/6056A61K2039/627A61K2039/55566
Inventor 孙恩珠李勇直李尚珉
Owner BIOAPPL
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