Sweetness sensitive food formula and animal model construction method thereof
A food and sweetness technology, applied in food science, animal feed, animal husbandry, etc., can solve the problems of unclear sweetness sensitivity and unanalyzed, and achieve the effect of increasing sweetness sensitivity
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[0034] Animal model: wild-type Drosophila W 1118 .
[0035] A large amount of this strain Drosophila (W 1118 ), the parameters of the constant temperature and humidity incubator for experimentally raising fruit flies are: temperature: 25±1°C; relative humidity 65%±5%; day and night cycle: 12h light (7:30~19:30) / 12h dark (19 :30~7:30).
[0036] Experimental reagents:
[0037] Table 1 Table of experimental reagents
[0038]
[0039] The conventional food formula of feeding and breeding experiment Drosophila: every 1L food contains water, agar (1%), yeast powder (10%), sucrose (5%), propionic acid (0.6%), 10% m / v Ni Parkin methyl ester-ethanol solution (1.2%).
[0040] Sweetness sensitivity detection method:
[0041] The sweetness sensitivity of Drosophila can be detected by proboscis extension response (PER) (Shiraiwa T, Carlson JR. Proboscis extension response (PER) assay in Drosophila. Journal of visualized experiments: JoVE, 2007, (3): 193-193) for detection. PER r...
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