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Detection membrane strip, detection card and detection kit for allergen detection

A technology for detecting kits and allergens, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of long operation time of ELISA method, inability to realize bedside detection, cumbersome operation, etc., to achieve qualitative detection and convenience Quantitative detection, the effect of accurate detection results

Pending Publication Date: 2021-12-24
山东康华生物医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned detection methods all have defects: radioallergen adsorption detection has nuclide pollution, and it needs to be equipped with instruments and professional personnel to operate; there are many methods for detecting binding enzymes in enzyme immunoassay, and the commonly used ELISA method takes a long time to operate, and Only one allergen can be detected per well, with high efficiency and low cost; immunoblotting has high sensitivity and can realize multi-throughput detection, but its operation is cumbersome and takes a long time; chemiluminescence has high sensitivity and can realize automatic operation, but it needs supporting equipment, and bedside detection cannot be realized, that is, random detection and detection

Method used

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  • Detection membrane strip, detection card and detection kit for allergen detection
  • Detection membrane strip, detection card and detection kit for allergen detection
  • Detection membrane strip, detection card and detection kit for allergen detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A detection membrane strip for allergen detection, comprising a nitrocellulose membrane coated with 8 groups of quality control lines and detection lines; each of the quality control lines is coated with goat anti-mouse Antibody, the coating concentration of sheep anti-mouse antibody is 3mg / mL, the allergen protein antigen coating forms the detection line, two kinds of allergen protein antigen coating form a detection line, the coating concentration of the allergen protein antigen 3mg / mL;

[0041] The allergen protein antigens include: milk allergen protein antigens, meat allergen protein antigens, egg allergen protein antigens, nut allergen protein antigens, grain allergen protein antigens, bean allergen protein antigens, fruit allergen protein antigens, Aquatic allergen protein antigen, cockroach allergen protein antigen, mold allergen protein antigen, pollen allergen protein antigen, inhalant mite allergen protein antigen, animal allergen protein antigen.

[0042] T...

Embodiment 2

[0057] Same as Example 1, except that: the nitrocellulose membrane is coated with 12 sets of quality control lines and detection lines; the coating concentration of goat anti-mouse antibody is 1 mg / mL, an allergen protein antigen Coating forms a detection line, and the coating concentration of the allergen protein antigen is 0.1 mg / mL.

Embodiment 3

[0059] Same as Example 1, except that: the nitrocellulose membrane is coated with 10 sets of quality control lines and detection lines; the coating concentration of goat anti-mouse antibody is 2 mg / mL, an allergen protein antigen Coating forms two detection lines, and the coating concentration of the allergen protein antigen is 1.5 mg / mL.

[0060] Of course, in addition to implementation 1, implementation 2 and implementation 3, the nitrocellulose membrane can be coated with different groups of quality control lines and detection lines according to needs; select the required allergen protein antigen coating to form a detection line.

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PUM

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Abstract

The invention provides a detection membrane strip, a detection card and a detection kit for allergen detection, which can be used for rapidly detecting whether a human body has lesion or immune tolerance reaction due to food, pollen, dust mites, mould and the like, the detection method is simple, convenient and rapid, and the detection result is accurate and reliable. Various allergic diseases caused by allergens to human bodies are avoided, different detection requirements are met, the cost is low, the structure is simple, assembly is easy, and the detection membrane strip, the detection card and the detection kit are suitable for large-scale production, can be suitable for whole blood detection, and are particularly suitable for self-inspection of infants and family members; the invention provides an immunofiltration device capable of limiting flow, and aims to enable liquid to only permeate through a target position, slow down the flow speed and improve the detection sensitivity. A formula of the sample diluent is provided, so that the treatment of a whole blood sample is facilitated, and the test is smoothly carried out; the invention provides the whole blood filter membrane, which is beneficial to filtering large-particle substances in a sample when detecting that the sample is whole blood, and is convenient for the sample to smoothly pass through the nitrocellulose membrane.

Description

technical field [0001] The invention relates to a detection device for allergen detection. [0002] Specifically, it relates to a detection film strip, a detection card and a detection kit for allergen detection. Background technique [0003] In recent years, the number of allergic patients is increasing, and the incidence of allergies is also increasing year by year. It is reported that 22% of the world's total population has allergic symptoms to varying degrees. Among the allergic reactions, more than 90% are caused by eight types of food, namely eggs, peanuts, milk, soybeans, wheat, tree nuts (nuts), shellfish (including crustaceans and molluscs), fish etc. Food allergy refers to a specific immune response that occurs in the body when the human body ingests a specific food. Food allergy is divided into IgE-mediated hypersensitivity and cell-mediated hypersensitivity (non-IgE-mediated hypersensitivity), and most food allergies are mediated by IgE. IgE-mediated allergic...

Claims

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Application Information

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IPC IPC(8): G01N33/532G01N33/533G01N33/558G01N33/68
CPCG01N33/6854G01N33/532G01N33/558G01N33/533
Inventor 杨金红杨帆许建成黎村艳王恩兰
Owner 山东康华生物医疗科技股份有限公司
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