Detection membrane strip, detection card and detection kit for allergen detection
A technology for detecting kits and allergens, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of long operation time of ELISA method, inability to realize bedside detection, cumbersome operation, etc., to achieve qualitative detection and convenience Quantitative detection, the effect of accurate detection results
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Embodiment 1
[0040] A detection membrane strip for allergen detection, comprising a nitrocellulose membrane coated with 8 groups of quality control lines and detection lines; each of the quality control lines is coated with goat anti-mouse Antibody, the coating concentration of sheep anti-mouse antibody is 3mg / mL, the allergen protein antigen coating forms the detection line, two kinds of allergen protein antigen coating form a detection line, the coating concentration of the allergen protein antigen 3mg / mL;
[0041] The allergen protein antigens include: milk allergen protein antigens, meat allergen protein antigens, egg allergen protein antigens, nut allergen protein antigens, grain allergen protein antigens, bean allergen protein antigens, fruit allergen protein antigens, Aquatic allergen protein antigen, cockroach allergen protein antigen, mold allergen protein antigen, pollen allergen protein antigen, inhalant mite allergen protein antigen, animal allergen protein antigen.
[0042] T...
Embodiment 2
[0057] Same as Example 1, except that: the nitrocellulose membrane is coated with 12 sets of quality control lines and detection lines; the coating concentration of goat anti-mouse antibody is 1 mg / mL, an allergen protein antigen Coating forms a detection line, and the coating concentration of the allergen protein antigen is 0.1 mg / mL.
Embodiment 3
[0059] Same as Example 1, except that: the nitrocellulose membrane is coated with 10 sets of quality control lines and detection lines; the coating concentration of goat anti-mouse antibody is 2 mg / mL, an allergen protein antigen Coating forms two detection lines, and the coating concentration of the allergen protein antigen is 1.5 mg / mL.
[0060] Of course, in addition to implementation 1, implementation 2 and implementation 3, the nitrocellulose membrane can be coated with different groups of quality control lines and detection lines according to needs; select the required allergen protein antigen coating to form a detection line.
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