Method for establishing fungal proteolysis peptide fingerprint spectrum

A fungal protease and fingerprint technology, which is applied in the field of identification of traditional Chinese medicine components, can solve the problems of little research and achieve the effect of good accuracy, high sensitivity and simple operation

Pending Publication Date: 2021-12-31
NAT INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Mycoprotein as one of the active ingredients, little research

Method used

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  • Method for establishing fungal proteolysis peptide fingerprint spectrum
  • Method for establishing fungal proteolysis peptide fingerprint spectrum
  • Method for establishing fungal proteolysis peptide fingerprint spectrum

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Experimental program
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Embodiment Construction

[0027] The principles and features of the present invention are described below, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0028] Instruments and reagents

[0029] Instruments: Ultra-High Performance Liquid Chromatography (Thermo, USA); Orbitrap Fusion Mass Spectrometer (Thermo, USA); EPS-301 Electrophoresis Instrument (General Electric, USA); Vacuum Centrifugal Concentrator.

[0030] Reagents: polyacrylamide, Coomassie brilliant blue, acetic acid, dithiothreitol (DTT), iodoacetamide (IAM), trifluoroacetic acid (TFA), ammonium bicarbonate, etc. are chemically or analytically pure; formic acid, acetonitrile All were chromatographically pure (Fisher, USA); deionized water was Millipore ultrapure water; trypsin was sequence pure.

[0031] Chromatographic conditions

[0032] Column: ZorbaxSB-C 18 (150mm×75mm, 3μm);

[0033] Mobile phase: A: 0.1% formic acid solution; B: 0.1% formic...

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Abstract

The invention discloses a method for establishing a fungal protein enzymolysis peptide fingerprint spectrum. The method comprises the following steps: extracting fungal protein; carrying out purification and enzymolysis on the fungal protein liquid; and carrying out UPLC-MS analysis on the fungal protein liquid subjected to enzymolysis, establishing a mass spectrum fingerprint spectrum according to a method under mass spectrum-chromatography conditions, and carrying out methodological investigation; wherein the mass spectrum fingerprint spectrum provides big data support for further searching characteristic ions of cordyceps sinensis and various fermented cordyceps sinensis preparations. The method disclosed by the invention has the advantages of simplicity in operation, high sensitivity, good accuracy and the like, the quality condition of the fungal protein enzymolysis peptide of each sample can be grasped from the overall characteristics of the mass spectrum, and a novel scientific method is provided for quality control and authenticity identification of cordyceps sinensis and various fermented cordyceps sinensis powder and preparations.

Description

technical field [0001] The invention belongs to the technical field of component identification of traditional Chinese medicines, and in particular relates to a method for establishing fingerprints of fungal proteolytic peptides. Background technique [0002] The composition of traditional Chinese medicine is complex, and using a certain composition as a control index is no longer suitable for the requirements of quality control of traditional Chinese medicine. The fingerprint of traditional Chinese medicine can not only judge the authenticity and quality of Chinese medicinal materials, but also judge the quality of traditional Chinese medicine preparations and the rationality of the preparation process. Compared with the quality control method in which a single component is used as a control index, fingerprints can fully reflect the quality of the sample from the overall chemical composition, thereby achieving the purpose of effective quality control and overcoming the disa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72
CPCG01N30/02G01N30/72
Inventor 张萍魏锋马双成康帅李娟刘薇李明华陆兔林
Owner NAT INST FOR FOOD & DRUG CONTROL
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