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Production of salipro particles

A technology of particles and lipoprotein particles, which is applied in the field of preparation of saposin lipoprotein particles, can solve the problems of non-providing, limited water solubility of hydrophobic agents, etc., and achieve the effect of easy freeze-drying

Pending Publication Date: 2021-12-31
塞利普洛生物技术有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The second challenge, namely, the administration and delivery of hydrophobic agents (e.g., hydrophobic compounds and / or proteins) as therapeutic or diagnostic agents is mainly due to the limited water solubility of hydrophobic agents.
Furthermore, the prior art does not offer an easy possibility to recycle excess particle components for another particle assembly reaction

Method used

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  • Production of salipro particles
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Embodiment Construction

[0063] The invention provides a method for producing saposin lipoprotein particles, wherein the saposin lipoprotein particles produced comprise

[0064] - saposin-like protein,

[0065] - Lipids, and

[0066] - an optional hydrophobic agent, wherein the hydrophobic agent is different from lipids, and

[0067] (1) wherein the method comprises the following steps:

[0068] a) providing a lipid and an optional hydrophobic agent;

[0069] b.1) contacting the saposin-like protein with a support capable of selectively binding the saposin-like protein to the support in a liquid environment;

[0070] c.1) contacting the support-bound saposin-like protein with a lipid and optionally a hydrophobic agent to enable self-assembly of saposin lipoprotein particles on the support;

[0071] d) optionally, eluting the support-bound saposin lipoprotein particles; or

[0072] (II) Wherein optionally, the method comprises the following steps:

[0073] a) providing hydrophobic agents and lipi...

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Abstract

The invention relates to a process for preparing saposin lipoprotein particles, comprising a saposin-like protein, lipids and optionally a hydrophobic agent wherein the saposin-like protein or the hydrophobic agent is selectively bound to a support to allow the self-assembly of the saposin lipoprotein particles. The process of the invention comprises the step of a.) providing the hydrophobic agent and lipids, b. 1) / b.2) contacting the hydrophobic agent or the saposin-like protein with a support that is capable of selectively binding either of the two molecules to the support, c.1) / c.2) contacting the support-bound particle components with the remaining particle components, either the saposin-like protein or the hydrophobic agent, to allow fpr the self-assembly of the saposin lipoprotein particle on the support and d.) optionally eluting the support-bound saposin lipoprotein particles.

Description

technical field [0001] The present invention relates to a method for preparing saposin lipoprotein particles comprising a saposin-like protein, a lipid and optionally a hydrophobic agent. Background technique [0002] Membranes surround all living cells and form the envelope of many viruses (eg, HIV). In living cells, the cell membrane acts as a semipermeable barrier to preserve the contents of the cell. Membrane components, especially membrane proteins such as receptors and transporters, also determine and regulate how a cell interacts with its environment. Functions that regulate how cells interact with their environment, including biological processes such as signal transduction, molecular transport, energy metabolism, formation of cell-cell contacts, and cellular homeostasis, are critical and depend on membrane proteins. About 30% of all ORFs encode membrane proteins (see "Wallin and von Heijne, Protein Science 1998 Apr; 7(4): 1029-38") and thus roughly represent a qua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/24A61K47/42
CPCA61K9/1274A61K9/1275A61K47/24A61K47/42
Inventor 罗宾·勒文延斯·弗劳恩菲尔德
Owner 塞利普洛生物技术有限责任公司
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