Molecular diagnosis and treatment preparation for endometriosis as well as preparation method and application thereof

A technology for endometriosis and ectopia, applied in the field of medical cell biology, can solve the problems of lack of efficient and direct tools and unclear pathogenesis, and achieve the effect of simple process and low cost

Pending Publication Date: 2022-01-21
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, because the pathogenesis of the disease is still unclear, there is still a lack of efficient and direct tools for the diagnosis and treatment of the disease. Early detection is undoubtedly a prerequisite for the

Method used

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  • Molecular diagnosis and treatment preparation for endometriosis as well as preparation method and application thereof
  • Molecular diagnosis and treatment preparation for endometriosis as well as preparation method and application thereof
  • Molecular diagnosis and treatment preparation for endometriosis as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Preparation of the polypeptide sequence described in SEQ ID No.1

[0043] The polypeptide sequence is industrially synthesized by a specialized polypeptide production company.

[0044] Based on resin-based solid-phase synthesis, polypeptide molecules are synthesized through condensation reactions between amino acids from the C-terminal to the N-terminal, and modified with azidoacetic acid.

Embodiment 2

[0045] Example 2: Preparation of molecular diagnosis and treatment preparations for endometriosis

[0046] Indocyanine green dye was selected as the effector component, purchased from a commercial chemical reagent company, and modified with a DBCO linker, so as to facilitate subsequent connection with the polypeptide sequence. For the targeting component part, the polypeptide sequence prepared in Example 1 was selected. Mix the effector component and the targeting component at a molar ratio of 1:0.5, mix thoroughly, and store at 4°C.

Embodiment 3

[0047] Embodiment 3: preparation of endometriosis mouse animal model

[0048] Take an adult female mouse, after disinfection and anesthesia, take the orthotopic endometrial tissue, cut into small pieces (2 mm X 2 mm), and inoculate it near the mesenteric blood vessel. After the wound is sutured, the mouse rests and drinks freely. Modeling was considered successful when vesicles grew after one month.

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Abstract

The invention discloses a molecular diagnosis and treatment preparation for endometriosis as well as a preparation method and application thereof. The preparation comprises: 1) an effect component, namely a diagnostic compound and/or a drug for treating endometriosis; 2) a targeting component, namely a polypeptide with targeting recognition capability; the polypeptide comprises a polypeptide formed by combining one or more of the following sequences, or a derivative of the polypeptide formed by combining one or more of the following sequences: SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5 and SEQ ID No. 6. The method is suitable for diagnosis and/or treatment of human endometriosis diseases, and can also be used as a research tool of an endometriosis modeling experimental animal.

Description

technical field [0001] The invention belongs to the field of medical cell biology, and relates to a molecular diagnosis and treatment preparation for endometriosis, a preparation method and application thereof. Background technique [0002] Endometriosis is a common gynecological intractable disease in which active endometrial tissue grows ectopically in a non-uterine environment. So far, because the pathogenesis of the disease is still unclear, there is still a lack of efficient and direct tools for the diagnosis and treatment of the disease. Early detection is undoubtedly a prerequisite for the intervention and treatment of the disease. It is a valuable idea for diagnosis, intervention and treatment to deliver reagents or drugs with diagnostic and therapeutic value to endometriosis lesions in the simplest way. Contents of the invention [0003] In order to solve the problems raised in the above-mentioned background technology, the object of the present invention is to p...

Claims

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Application Information

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IPC IPC(8): A61K47/64A61K47/60A61K49/00A61K51/08A61K31/57A61K31/58A61K38/09A61P15/00
CPCA61K47/64A61K47/60A61K49/0056A61K51/08A61K31/57A61K31/58A61K38/09A61P15/00A61K49/00A61K31/337A61K31/704A61K35/00A61K45/00A61K49/04A61K49/14A61K49/22A61P35/00C07K14/445C07K17/00
Inventor 赵华山张键
Owner SHENZHEN INST OF ADVANCED TECH
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