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Method for determining geosmin and dimethyl isoborneol in fish body based on rapid pretreatment technology

A technology of dimethylisoborneol and geosmin, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of increased processing and extraction time, increased process, increased detection cost, etc., and achieves low matrix interference and fast analysis speed , the effect of using less

Active Publication Date: 2022-01-21
DALIAN OCEAN UNIV +1
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  • Claims
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Problems solved by technology

However, in addition to the target GSM and 2-MIB being extracted during n-hexane extraction, its lipophilicity will also lead to the extraction of fat and lipophilic compounds in fish tissues, so it is necessary to increase the SPE column purification process during operation, resulting in The processing and extraction time increases, the process increases, and the detection cost increases

Method used

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  • Method for determining geosmin and dimethyl isoborneol in fish body based on rapid pretreatment technology
  • Method for determining geosmin and dimethyl isoborneol in fish body based on rapid pretreatment technology
  • Method for determining geosmin and dimethyl isoborneol in fish body based on rapid pretreatment technology

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Embodiment 1

[0052] (1) Liquid-solid dispersion extraction: Weigh 2.0g sample, place it in a 20mL polypropylene centrifuge tube, add 2mL water, vortex for 30s, disperse the tissue sample, add 5mL acetonitrile extraction solution, vortex for 30s, add Back-extract with 1g of sodium chloride, vortex for 30s, add 4g of anhydrous sodium sulfate, centrifuge at 8000r / min for 5min, transfer the supernatant to a 20mL polypropylene centrifuge tube for purification.

[0053] (2) Liquid-solid adsorption purification: add 0.1g PSA powder and 0.1g C18 powder to the supernatant, vortex for 30s, centrifuge at 8000r / min for 1min, take the supernatant, pass through a microporous membrane, and wait for analysis.

[0054] (3) Standard solution preparation: First, weigh 0.01 g (accurate to 0.0001 g) of GSM and 2-MIB, respectively, and dilute to 10 mL with methanol to prepare a 1 mg / mL standard stock solution. Measure appropriate amount of GSM and 2-MIB respectively, and prepare a mixed standard intermediate so...

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Abstract

The invention belongs to the technical field of aquaculture and food monitoring, discloses a method for determining geosmin and dimethylisoborneol in a fish body based on a rapid pretreatment technology, and establishes a detection method for determining GSM and 2MIB in fish tissues through dispersive solid phase extraction-gas chromatography-mass spectrometry. The method comprises the following steps: extracting a sample with an acetonitrile solution, and performing salting-out assisted extraction; after purification, under a gradient heating condition, subjecting a target analyte to qualitative analysis by comparing retention time and ion pair information in a selected ion monitoring mode, and performing quantification by an internal standard method. The method has the advantages of small required sample amount and reagent amount, high analysis speed, high sensitivity and good reproducibility. The method can meet the large-batch detection requirements of GSM and 2MIB in fish tissue samples, and provides a rapid detection technical guarantee for evaluating the occurrence condition of earthy smell substances in fish tissues and exploring the reduction and removal of typical earthy smell substances in fish bodies.

Description

technical field [0001] The invention relates to a method for measuring soil odor substances in fish bodies based on rapid pretreatment, belongs to the technical field of aquaculture and food monitoring, and mainly relates to freshwater fish and seawater fish and the like. Mainly based on dispersive solid-phase extraction technology, the improved dispersive extraction and purification methods are used to complete the effective extraction of geosmin and dimethylisoborneol in the sample, and then perform quantitative analysis by GC / MS, which can realize the in vivo analysis of farmed fish and wild fish. The rapid extraction and detection of geosmin and dimethylisoborneol can effectively save analysis time and cost. Background technique [0002] The problem of earthy smell in aquatic products has a long history. Earthy smell substances in aquatic products have a profound impact on the breeding, processing, sales and consumption of aquatic products, and will cause serious economi...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/14
CPCG01N30/88G01N30/06G01N30/14G01N2030/025G01N2030/062G01N2030/146G01N2030/884Y02A40/81
Inventor 曲宝成王侯宇谢晴赵洪霞刘鹰岳扬王炫凯张敬波
Owner DALIAN OCEAN UNIV
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