Eurotium cristatum culture medium and eurotium cristatum plate counting method

A technology of Rhizoctonia coronoidans and culture medium, applied in microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve problems such as confusion, inaccuracy, safety accidents, etc., and achieve the effect of inhibiting bacteria

Pending Publication Date: 2022-02-01
漳州海关综合技术服务中心
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Problems solved by technology

Fuzhuan tea is a unique tea variety in China. According to the current national standard GB/T9833.3-2013, the qualified standard of Fuzhuan tea is that the number of coronoid cysts ≥ 200,000 CFU/g, but there is no detection of coronoid cysts at home and abroad. Therefore, GB/T 9833.3 can only temporarily specify the standard GB4789.15 (National Food Inspection Standard Mold and Yeast Count) to detect S. coronoid; GB4789.

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  • Eurotium cristatum culture medium and eurotium cristatum plate counting method
  • Eurotium cristatum culture medium and eurotium cristatum plate counting method

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[0056] In order to make the techniques, creative characteristics of the present invention, it is easy to understand that the present invention is further illustrated in connection with the specific embodiments.

[0057] See Figure 1 to 2 The present invention provides a technical solution: a culture medium of crown proximal cytoplasma, its composition and content: protein 胨 5g, glucose 10g, sucrose 20g, potassium phosphate 1g, magnesium sulfate 0.5g, agar 15g, bengal 0.03 g, chloramphenicol 0.1 g, sodium chloride 10% -15%, lithium chloride 6g, each component mixed, adjusted pH to 7.2 ± 0.2.

[0058] A crown projection cystic flat plate count method, the specific steps are as follows:

[0059] Step 1: Preparation of the sample; select the appropriate amount of tea sample standby, take the right amount of crown protrusion cystic medium raw material spare;

[0060] Step 2: Samples of the sample; crush the tea sample with a sterile pulverizer, and weigh the sample spare after the quad...

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Abstract

The invention provides an eurotium cristatum culture medium and an eurotium cristatum plate counting method, the eurotium cristatum culture medium comprises the following components by weight: 3-7 g of peptone, 8-12 g of glucose, 18-23 g of sucrose, 0.7-1.3 g of potassium dihydrogen phosphate, 0.2-0.7 g of magnesium sulfate, 13-18 g of agar, 0.01-0.05 g of rose-bengal, 0.05-0.2 g of chloramphenicol, 10-15% of sodium chloride and 5-7 g of lithium chloride, the components are mixed, and the pH value is adjusted to 7.2 +/-0.2. The novel eurotium cristatum culture medium can inhibit bacteria, control the growth of part of fungi and control the spreading of bacterial colonies, and on the eurotium cristatum culture medium, eurotium cristatum has relatively unique bacterial colony characteristics and can be easily separated from other fungi, through a subsequent corresponding eurotium cristatum plate counting method, the problem that no special quantitative counting method for eurotium cristatum in Fuzhuan tea exists at present can be solved.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a culture medium for S. coronoidis and a plate counting method for S. coronis. Background technique [0002] The most characteristic of dark tea is Fuzhuan brick tea. The probiotic Saccharomyces coronis in Fuzhuan brick tea has an important influence on the tea quality of Fuzhuan brick tea. Fuzhuan tea is a unique tea variety in China. According to the current national standard GB / T9833.3-2013, the qualified standard of Fuzhuan tea is that the number of coronoid cysts ≥ 200,000 CFU / g, but there is no detection of coronoid cysts at home and abroad. Therefore, GB / T 9833.3 can only temporarily specify the standard GB4789.15 (National Food Inspection Standard Mold and Yeast Count) to detect S. coronoid; GB4789.15 uses potato dextrose agar or Bengal red medium Cultivate mold. The above two kinds of mold culture media do not have any selectivity for mold. Alth...

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Application Information

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IPC IPC(8): C12N1/14C12Q1/06C12R1/645
CPCC12N1/14C12Q1/06
Inventor 周赞虎黄伙水林扬闻徐敦明曾静庄燕煌
Owner 漳州海关综合技术服务中心
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