Eurotium cristatum culture medium and eurotium cristatum plate counting method

Abstract

The invention provides an eurotium cristatum culture medium and an eurotium cristatum plate counting method, the eurotium cristatum culture medium comprises the following components by weight: 3-7 g of peptone, 8-12 g of glucose, 18-23 g of sucrose, 0.7-1.3 g of potassium dihydrogen phosphate, 0.2-0.7 g of magnesium sulfate, 13-18 g of agar, 0.01-0.05 g of rose-bengal, 0.05-0.2 g of chloramphenicol, 10-15% of sodium chloride and 5-7 g of lithium chloride, the components are mixed, and the pH value is adjusted to 7.2 + / -0.2. The novel eurotium cristatum culture medium can inhibit bacteria, control the growth of part of fungi and control the spreading of bacterial colonies, and on the eurotium cristatum culture medium, eurotium cristatum has relatively unique bacterial colony characteristics and can be easily separated from other fungi, through a subsequent corresponding eurotium cristatum plate counting method, the problem that no special quantitative counting method for eurotium cristatum in Fuzhuan tea exists at present can be solved.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a culture medium for S. coronoidis and a plate counting method for S. coronis. Background technique [0002] The most characteristic of dark tea is Fuzhuan brick tea. The probiotic Saccharomyces coronis in Fuzhuan brick tea has an important influence on the tea quality of Fuzhuan brick tea. Fuzhuan tea is a unique tea variety in China. According to the current national standard GB / T9833.3-2013, the qualified standard of Fuzhuan tea is that the number of coronoid cysts ≥ 200,000 CFU / g, but there is no detection of coronoid cysts at home and abroad. Therefore, GB / T 9833.3 can only temporarily specify the standard GB4789.15 (National Food Inspection Standard Mold and Yeast Count) to detect S. coronoid; GB4789.15 uses potato dextrose agar or Bengal red medium Cultivate mold. The above two kinds of mold culture media do not have any selectivity for mold. Alth...

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Problems solved by technology

Fuzhuan tea is a unique tea variety in China. According to the current national standard GB / T9833.3-2013, the qualified standard of Fuzhuan tea is that the number of coronoid cysts ≥ 200,000 CFU / g, but there is no detection of coronoid cysts at home and abroad. Therefore, GB / T 9833.3 can only temporarily specify the standard GB4789.15 (National Food Inspection Standard Mold and Yeast Count) to detect S. coronoid; GB4789.15 uses potato dextrose agar or Bengal red medium Cultivate mold. The above two kinds of mold culture media do not have any selectivity for mold. Although Cystis coronis belongs to mold, there are many pathogenic bacteria in mold and yeast. It is also yellow on the plate, which is very easy to confuse. It is not only inaccurate to detect S. coronoidis by counting mold and yeast, but also may cause major safety accidents.

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