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Method for rapidly identifying in-vitro hydroponic salt tolerance of plants

A salt-tolerant, plant-based technology, applied in the agricultural field, can solve the problem of consuming more resources, and achieve the effect of less resource consumption, accurate identification, and simple and easy-to-operate methods

Pending Publication Date: 2022-02-08
河北省农林科学院滨海农业研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although there are many methods for identifying plant salt tolerance, most of them consume more resources and take a certain amount of time. There is an urgent need for a fast, accurate, simple and easy-to-operate method for evaluating plant salt tolerance

Method used

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  • Method for rapidly identifying in-vitro hydroponic salt tolerance of plants
  • Method for rapidly identifying in-vitro hydroponic salt tolerance of plants
  • Method for rapidly identifying in-vitro hydroponic salt tolerance of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Test materials

[0036] The test material is Tamarix.

[0037] 2. Test method

[0038] A total of 6 NaCl treatments were set up in the experiment, the concentration gradients were 0 (CK), 1.0%, 1.5%, 2.0%, 2.5% and 3.0%, and each treatment was repeated 3 times. Quantitatively put the configured NaCl solution into the triangular flask, cut the branches of Tamarix tamarisk in the same year, which grow vigorously, have no pests and diseases, and grow in the same way, cut them into consistent lengths (28-30cm), insert them into different concentrations of NaCl solutions and carry out salt stress treatment, respectively The phenotype was observed at 3d, 5d and 7d after the salt treatment, and the relative electrical conductivity of the leaves of the plants under different treatments was measured. Three leaves of the same part were selected for each treatment, and the test was repeated 3 times, and the average value was calculated.

[0039] The steps for measuring the re...

Embodiment 2

[0057] 1. Test materials

[0058] The test material is elderberry.

[0059] 2. Test method

[0060] A total of 6 NaCl treatments were set up in the experiment, the concentration gradients were 0 (CK), 0.2%, 0.4%, 0.6%, 0.8% and 1.0%, and each treatment was repeated 3 times. Quantitatively put the configured NaCl solution into the triangular flask, cut the branches of Elderberry that grow vigorously, have no pests and diseases, and grow in the same way, cut them into uniform lengths (28-30cm), and insert them into NaCl solutions of different concentrations for salt stress treatment. The phenotype was observed at 3d, 5d and 7d after salt treatment, and the relative electrical conductivity of elderberry leaves under different treatments was measured. Three leaves of the same part were selected for each treatment, and the experiment was repeated 3 times, and the average value was calculated.

[0061] The steps for measuring the relative conductivity of the leaves are as follows:...

Embodiment 3

[0079] 1. Test materials

[0080] The test material is cut chrysanthemum.

[0081] 2. Test method

[0082]A total of 6 NaCl treatments were set up in the experiment, the concentration gradients were 0 (CK), 0.2%, 0.4%, 0.6%, 0.8% and 1.0%, and each treatment was repeated 3 times. Quantitatively put the configured NaCl solution into the triangular flask, cut the branches of the cut chrysanthemum that are healthy, free from diseases and insect pests, and grow in the same way, cut into consistent lengths (28-30cm), and insert them into different concentrations of NaCl solutions for salt stress treatment. The phenotype was observed at 3d, 5d and 7d after the salt treatment, and the relative conductivity of the leaves of cut chrysanthemum under different treatments were measured. Three leaves of the same part were selected for each treatment, and the experiment was repeated 3 times, and the average value was calculated.

[0083] The steps of measuring the relative conductivity of...

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Abstract

The invention relates to a method for rapidly identifying in-vitro hydroponic salt tolerance of plants, and belongs to the technical field of agriculture. According to the technical scheme, the method comprises the following steps: preparing a plurality of triangular flasks, preparing salt treatment solutions with different NaCl concentrations according to different plant types, and quantitatively adding the salt treatment solutions into the prepared triangular flasks; selecting branches which grow robustly in the current year, are free from plant diseases and insect pests and are consistent in growth vigor, shearing the branches into branches with consistent height of 28-30cm, and inserting the branches into the triangular flask filled with a NaCl solution for salt stress treatment; respectively observing plant phenotypes in 3d, 5d and 7d after salt treatment, measuring the relative conductivity of plant leaves under different salt treatment, selecting three leaves at the same part for measurement in each treatment, repeating the test for three times, and calculating an average value. The method has the beneficial effects that less resources are consumed, and the method is simple, convenient and easy to operate; the culture mode is water culture, and the salt stress concentration is relatively constant; and 7 days after the salt treatment test is finished, the plant salt tolerance can be quickly and accurately identified.

Description

technical field [0001] The invention relates to a method for rapid identification of salt tolerance of isolated hydroponic plants, which belongs to the field of agricultural technology. Background technique [0002] Soil salinization is a worldwide ecological and environmental problem. In my country's northeast, north, northwest inland areas and coastal areas north of the Yangtze River, saline-alkali soil is widely distributed, seriously restricting the development of economy and agriculture. Excessive salinity in the soil will not only inhibit plant growth, but also cause osmotic stress, ion poisoning, and secondary stress, which will inhibit energy production and affect metabolic activities, seriously threatening the normal growth and development of plants. However, different plants have different response mechanisms and sensitivity to salt stress. Under salt conditions, plants can avoid or reduce the damage caused by salt stress by increasing the activity of protective e...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G7/06
CPCA01G31/00A01G7/06
Inventor 郭艳超丁丁郑伶杰王红宝韩民利马立军刘宝轻吴新海
Owner 河北省农林科学院滨海农业研究所
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