Fermentation medium and method for fermentation production of spinosad

Abstract

The invention relates to a fermentation medium and a method for fermentation production of spinosad. The fermentation medium comprises a fermentation basic medium, a fermentation supplementary medium I and a fermentation supplementary medium II; the fermentation basic medium is prepared from: 7.5-8% of glucose, 2.5-3% of cottonseed meal, 0.5-1% of yeast extract, 0.5-1% of corn steep liquor, 0.5-1% of peptone, 0.2-0.4% of potassium dihydrogen phosphate, 0.2-0.3% of calcium carbonate, 0.5-1% of ammonium sulfate, 0.0002-0.0005% of manganese sulfate, 0.0002-0.0005% of sodium molybdate, 0.0001-0.0002% of ferrous sulfate, 0.5-1% of soybean oil and 0.015-0.03% of a defoaming agent; the supplementary medium I comprises: 30% of corn steep liquor, 2% of potassium dihydrogen phosphate and 2% of sodium chloride; and the supplementary medium II comprises 65% of glucose and 0.5% of potassium dihydrogen phosphate. According to the invention, the spinosad production level of the saccharopolyspora spinosa is obviously improved.

Description

Technical field [0001] The present invention belongs to the field of fermentation, and more particularly to a method of fermentation medium and a fermentation of multi-bacteria. Background technique [0002] Multiplexin is the intracellular metabolite of the bisteosteoic bacteria, which belongs to the lactone antibiotics, which is broad and safe for mammals, birds, fish or even most vestibration due to efficient and low residues. The advantages such as boundaries, and many "Presidential Green Chemical Challenge Awards" have been obtained. [0003] However, there is currently the problem that the fermentation of multi-deficitin is that the conventional fermentation mode is low, and the pose of the bisteosteogeneous bacteria is low, and the production potential of the bisteosteoquence can not be effectively exported. Inventive content [0004] It is an object of the present invention to overcome the defects in the prior art, providing a method of fermentation medium and fermentati...

AI Technical Summary

Problems solved by technology

[0003] However, the current problem in the fermentation production of spinosyn is that under the traditional fermentation method, the production level of S. spinosa is low, and the production potential of S. spinosa cannot be effectively exerted.