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Acid-producing bacterium JC-H and application thereof as well as culture and identification method of acid-producing bacterium JC-H

An identification method and technology of acid-producing bacteria, applied in the field of environmental microorganisms, can solve the problems of a lot of manpower, material and financial resources, difficult for the public to accept, and high cost.

Pending Publication Date: 2022-02-11
XIAN UNVERSITY OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the actual repair process: physical methods mainly include soil replacement, heat treatment, electric repair, etc. However, physical methods generally require a lot of manpower, material resources, financial resources, etc., and are generally limited to small areas of heavy metal pollution treatment; chemical The methods mainly include leaching, immobilization of modifiers, precipitation, ion exchange and other methods. Although the chemical repair method is simple to operate and can also be repaired in situ, it generally brings secondary pollution and even destroys the soil structure, and is costly.
Therefore, these methods are difficult to realize technically and economically, and are also difficult to be accepted by the public. In the face of large-scale heavy metal pollution, it is even more difficult to apply

Method used

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  • Acid-producing bacterium JC-H and application thereof as well as culture and identification method of acid-producing bacterium JC-H
  • Acid-producing bacterium JC-H and application thereof as well as culture and identification method of acid-producing bacterium JC-H
  • Acid-producing bacterium JC-H and application thereof as well as culture and identification method of acid-producing bacterium JC-H

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Embodiment Construction

[0046] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0047] An acid-producing bacterium JC-H, the strain is named: Aspergillus niger (Aspergillus niger) JC-H, which was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on January 04, 2021, and the preservation number is CGMCC NO : 21424.

[0048] The cultivation method of acid producing bacteria JC-H is specifically:

[0049] Step 1, isolation and cultivation of bacterial strains

[0050] The soil heavily polluted by heavy metals was collected as a sample; the purpose was to isolate microorganisms with acid-producing ability from it.

[0051] Preparation of Chase medium: 30 g of sucrose, NaNO 3 3g,K 2 HPO 4 1g, KCl 0.5g, FeSO 4 ·7H 2 O 0.01g, 20g of agar, 1L of distilled water, then pour it into a plate and wait for it to solidify to make a sterile separation medium for later use; ...

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Abstract

The invention discloses an acid-producing bacterium JC-H, which is named as Aspergillus niger JC-H, is preserved in China General Microbiological Culture Collection Center (CGMCC) on January 4, 2021, and has a preservation number of CGMCC NO: 21424. The strain can be applied to acid production, and the acid production efficiency is the highest when a fermentation medium is used for constant-temperature shake culture under the conditions that the temperature is 30 DEG C, the pH is 6 and the rotating speed is 160rpm. The acid-producing bacterium provided by the invention can provide a strain resource for biological acid production.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, relates to an acid-producing bacterium JC-H and its application, and also relates to a method for cultivating and identifying the acid-producing bacterium JC-H. Background technique [0002] Mineral resources are the material basis for human production, life and rapid social and economic development, as well as strategic resources for ensuring national security and safeguarding national development interests. However, unreasonable mining will cause great damage to the mining area and the surrounding natural environment. The annual discharge of metal and non-metal tailings reaches 300 million tons, and the cumulative stock of tailings is nearly 20 billion tons. The tailings and waste residues in the mining area are accumulated in the natural environment, which increases the types and quantities of heavy metals in the tailings that penetrate into the soil environment, and conti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/02C12P7/40C12Q1/686C12Q1/04G01N31/16C12R1/685
CPCC12N1/14C12N1/02C12P7/40C12Q1/686C12Q1/04G01N31/16C12Q2533/101
Inventor 高天鹏王雪莹刘圆李广文张九东申圆圆其他发明人请求不公开姓名
Owner XIAN UNVERSITY OF ARTS & SCI
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