Acid-producing bacterium JC-H and application thereof as well as culture and identification method of acid-producing bacterium JC-H
An identification method and technology of acid-producing bacteria, applied in the field of environmental microorganisms, can solve the problems of a lot of manpower, material and financial resources, difficult for the public to accept, and high cost.
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[0046] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0047] An acid-producing bacterium JC-H, the strain is named: Aspergillus niger (Aspergillus niger) JC-H, which was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on January 04, 2021, and the preservation number is CGMCC NO : 21424.
[0048] The cultivation method of acid producing bacteria JC-H is specifically:
[0049] Step 1, isolation and cultivation of bacterial strains
[0050] The soil heavily polluted by heavy metals was collected as a sample; the purpose was to isolate microorganisms with acid-producing ability from it.
[0051] Preparation of Chase medium: 30 g of sucrose, NaNO 3 3g,K 2 HPO 4 1g, KCl 0.5g, FeSO 4 ·7H 2 O 0.01g, 20g of agar, 1L of distilled water, then pour it into a plate and wait for it to solidify to make a sterile separation medium for later use; ...
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