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Preparation method and application of gentiana scabra extract gentiopicroside

A technology of gentiopicroside and gentian grass, applied in the field of preparation of gentiopicroside extract from gentian grass, can solve the problems of difficult industrial production, low production cost, high production cost, etc., and achieve solvent-free and Pigment residue, easy to realize industrialization, and low production cost

Pending Publication Date: 2022-02-18
HENAN KANGHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the extraction and separation process of the crude product of gentiopicroside, these refining and purification methods have the advantages of high efficiency and high purity of gentiopicroside in the product. However, the above-mentioned refining methods have higher requirements on process conditions, higher production costs, and The output is low and the operation is complicated; in addition, toxic organic solvents need to be used, the production process is more polluting to the environment, and problems such as organic solvent residues in the product and dark color of the final product are easy to appear in the product, it is difficult to realize industrial production, and it is generally used in the laboratory In addition, there are some existing preparation methods, although the requirements for process conditions are not high, the production cost is not high, the output is large, the operation is not complicated, and the pollution to the environment is not large; however, in the final product The content of gentiopicroside is low, which does not meet the requirements of traditional Chinese medicine extract monomers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Firstly, the rhizome of Gentiana is crushed, then added with pure water for extraction, and then the extract is filtered and concentrated to obtain a concentrate; then the concentrate obtained in the above steps is filtered, and then the filtrate is poured into a macroporous resin, Then add water for elution, discharge the eluent, then pour ethanol for elution, collect the eluent, and recover the ethanol, and then obtain the ointment; finally add the ointment obtained in the above steps to silica gel, stir evenly, Put it on a silica gel column, elute with a mobile phase of ethyl acetate, ethanol and water with a volume ratio of 1:(1-2):(1-3), elute until the thin layer detects that there is no gentiopicroside, collect the eluted liquid, concentrated to 1 / 3-1 / 2 of the volume of the eluent, left to stand for crystallization, filtered, and the filter cake was washed with ethanol and dried to obtain gentiopicroside.

Embodiment 2

[0024] In embodiment one, add following operation:

[0025] The water in step 1 is pure water, and the volume of water is 7-9 times of the rhizome of gentian.

[0026] Firstly, the rhizome of Gentiana is crushed, then added with pure water for extraction, and then the extract is filtered and concentrated to obtain a concentrate; then the concentrate obtained in the above steps is filtered, and then the filtrate is poured into a macroporous resin, Then add water for elution, discharge the eluent, then pour ethanol for elution, collect the eluent, and recover the ethanol, and then obtain the ointment; finally add the ointment obtained in the above steps to silica gel, stir evenly, Put it on a silica gel column, elute with a mobile phase of ethyl acetate, ethanol and water with a volume ratio of 1:(1-2):(1-3), elute until the thin layer detects that there is no gentiopicroside, collect the eluted liquid, concentrated to 1 / 3-1 / 2 of the volume of the eluent, left to stand for crys...

Embodiment 3

[0028] In embodiment two, add following operation:

[0029] In the first step, the extraction needs to be carried out 3-5 times, and the concentrated solution needs to be concentrated to a relative density of 1-1.1 under the condition of 50-60°C.

[0030] Firstly, the rhizome of Gentiana is crushed, then added with pure water for extraction, and then the extract is filtered and concentrated to obtain a concentrate; then the concentrate obtained in the above steps is filtered, and then the filtrate is poured into a macroporous resin, Then add water for elution, discharge the eluent, then pour ethanol for elution, collect the eluent, and recover the ethanol, and then obtain the ointment; finally add the ointment obtained in the above steps to silica gel, stir evenly, Put it on a silica gel column, elute with a mobile phase of ethyl acetate, ethanol and water with a volume ratio of 1:(1-2):(1-3), elute until the thin layer detects that there is no gentiopicroside, collect the elu...

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Abstract

The invention discloses a preparation method and application of gentiana scabra extract gentiopicroside, and the preparation method comprises the following steps: S1, taking gentiana scabra rhizome, conducting crushing, then adding pure water for extraction, and then filtering and concentrating an extracting solution to obtain a concentrated solution; and S2, filtering the concentrated solution obtained in the previous step. According to the preparation method designed by the invention, the low-toxicity ethanol-water solution is used as a solvent for extracting and separating the gentiopicroside in the gentiana scabra, so that the preparation method is green and environment-friendly, and almost has no influence on the health of an operator; and besides, technical means used in the extraction and separation process of gentiopicroside comprise an ethanol-water solvent reflux extraction method and a macroporous adsorption resin method which are easy to industrialize, the operation is simple, the production cost is low, and on the premise that the extraction and separation process is green and environment-friendly, the operation is simple, the production cost is low and industrialization is easy to realize, and a gentiopicroside single body with good stability and no solvent and pigment residue is prepared.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicinal materials, in particular to a preparation method and application of gentiopicroside, an extract of Gentiana gentiana. Background technique [0002] There are more than 500 species of Gentianae Gentianae plants in the world, among which the tropical alpine and temperate regions are widely distributed. This kind of plants are also widely distributed in my country, with more than 230 species. The southwest is its main production area. Thirty-five species, Gentiana barbata, also known as Gentiana and Gentiana root, are perennial herbaceous plants under the genus Gentiana in the family Gentianaceae. Mainly produced in Heilongjiang area. The roots and rhizomes are used as medicine, bitter in taste and cold in nature, and have the effects of clearing away heat, purging fire and invigorating the stomach. Indications of gastritis, indigestion, jaundice and other diseases. Modern stu...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/04
CPCC07H1/08C07H17/04
Inventor 行建敏
Owner HENAN KANGHUA PHARMA
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