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Application of melatonin in delaying in-vitro differentiation of bovine follicular granulosa cells

A technology of melatonin and granulosa cells, which is applied in the application field of melatonin in delaying the differentiation of bovine follicular granulosa cells in vitro, can solve the problem of uncertain recovery of melatonin, and achieve the effect of delaying the differentiation of granulosa cells

Pending Publication Date: 2022-02-18
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether the effect of ACTH on the secretion of steroid hormones in granulosa cells can be restored, and the effect of melatonin on the restoration of the secretion of steroid hormones in granulosa cells is still uncertain, and there is no research report on this aspect at present

Method used

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  • Application of melatonin in delaying in-vitro differentiation of bovine follicular granulosa cells
  • Application of melatonin in delaying in-vitro differentiation of bovine follicular granulosa cells
  • Application of melatonin in delaying in-vitro differentiation of bovine follicular granulosa cells

Examples

Experimental program
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Embodiment 1

[0028] Example 1 Effect of melatonin on bovine follicular granulosa cell steroid hormone synthesis

[0029] 1. Cell Culture

[0030] The ovaries were collected at the slaughterhouse and placed in normal saline containing 100 IU / mL penicillin, 100 IU / mL streptomycin and 0.9% sodium chloride, and transported back to the laboratory within 2 hours in a thermos at 37°C. Granulosa cells are isolated from medium-sized follicles with a diameter of 3 to 8 mm. The follicular blood vessels are required to be clear and the follicular fluid transparent. Use a 10mL syringe to extract the fluid in the 3-8mm follicle from the surface of the bovine ovary obtained from the slaughterhouse. Collect the follicular fluid in a 15mL centrifuge tube, then centrifuge at 500g for 5min, the cells settle at the bottom of the centrifuge tube, and remove the supernatant. Resuspend the cells with PBS buffer and repeat the centrifugation 4 times. The cells were resuspended in a mixed culture solution cont...

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Abstract

The invention provides a new application of melatonin, and particularly relates to an application of melatonin in delaying in-vitro differentiation of bovine follicular granulosa cells. When an in-vitro culture solution added with melatonin is used for culturing the bovine follicular granule cells, the melatonin can inhibit synthesis of progesterone of the bovine follicular granulosa cells. Melatonin is used as an endocrine regulation factor of the bovine ovarian function, and the adrenocorticotropin (ACTH) is inhibited by down-regulating the synthesis of steroid hormone, so that the granulosa cell differentiation is delayed. Melatonin can become a preparation medicine for inhibiting synthesis of bovine follicular granulosa cell steroid, can be popularized and applied in animal husbandry production, and has good market effects and social effects.

Description

technical field [0001] The invention belongs to the technical field of livestock gamete embryo engineering, and in particular relates to the application of melatonin in delaying differentiation of bovine follicular granulosa cells in vitro. Background technique [0002] Follicular granulosa cells are the most important somatic cells in the follicle, which play an important role in the regulation of the local microenvironment of the follicle. Early granulosa cells are differentiated from follicular epithelial cells. Granulosa cells, as important steroid hormone synthesis cells, can promote the synthesis and secretion of estrogen, progesterone and other hormones and growth factors in the ovary, and regulate the growth, differentiation and maturation of follicular theca cells and oocytes through gap junctions, thereby regulating the growth and development of follicles. Development, maturation, ovulation, and follicular atresia. [0003] Melatonin, N-acetyl-5-methoxytryptamine...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0682C12N2501/825C12N2501/855C12N2501/105
Inventor 许晓玲杨婷刘彦白佳桦冯涛秦玉圣宋玉清肖霖力
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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