Molecular marker for diagnosing primary biliary cholangitis and application thereof
A molecular marker, cholangitis technology, applied in the field of molecular biology, can solve the problems of PBC dependence and misdiagnosis, and achieve the effect of reducing missed diagnosis and misdiagnosis, simple sampling and less trauma
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Embodiment 1
[0035] Example 1: To clarify the expression and localization of human multimeric immunoglobulin receptors in the liver tissues of CTR, PBC, OC, SSC and NASH patients.
[0036] One case of liver tissue samples remaining after surgical resection or clinicopathological diagnosis of patients with CTR, PBC, OC, SSC and NASH in the inpatient department of Southwest Hospital was collected. The liver tissue samples were fixed with paraformaldehyde, dehydrated and transparent, embedded in wax and then sliced. The thickness of the sliced tissue was 4um. Multiple immunohistochemical fluorescence technology was used to detect the expression and localization of human multimeric immunoglobulin receptors in the liver tissue of the subject. The reagent used was a multiple immunohistochemical fluorescence kit (Absin, abs50014). The specific steps were as follows:
[0037] (1) Dewaxing to water: Paraffin slices were baked in a 65-degree oven for 1 hour; slices were soaked in fresh xylene for ...
Embodiment 2
[0052] Example 2: Determining the expression of serum anti-human multimeric immunoglobulin receptor antibodies in patients with CTR, PBC, and OC.
[0053] Peripheral blood samples from 12 CTR, 17 PBC, and 15 OC patients were collected from the inpatient department of Southwest Hospital, and the expression of anti-human multimeric immunoglobulin receptor antibody in the serum of the subjects was detected by enzyme-linked immunosorbent assay (ELISA) , the reagent used is human multimer immunoglobulin receptor antibody ELISA kit (Lun Changshuo Bio, 100712), and the specific steps are:
[0054] (1) Take serum:
[0055] Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or overnight at 4°C, then centrifuge at 1000×g for 20 minutes, then take the supernatant, or store the supernatant at -20°C or -80°C to avoid Freeze and thaw repeatedly.
[0056] (2) Preparation of reagents and equipment:
[0057] Microwell microtiter plate, standa...
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