Bone marrow mesenchymal stem cell/degradable hydrogel composite material as well as preparation method and application thereof

A technology of bone marrow mesenchyme and composite material is applied in the field of bone marrow mesenchymal stem cell/degradable hydrogel composite material and its preparation, which can solve the problems of difficult matching of tissue regeneration rate and difficulty in providing tissue microenvironment responsiveness, etc. Achieve the effect of avoiding oxidative damage, protecting oxidative stress damage and eliminating reactive oxygen species

Pending Publication Date: 2022-03-08
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the existing cell-encapsulated hydrogel is difficult to provide tissue microenvironment responsiveness to protect the loaded cells and the degradation rate is difficult to match with the tissue regeneration rate in vivo, and to provide a bone marrow mesenchymal Stem cell / degradable hydrogel composite material and its preparation method and application

Method used

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  • Bone marrow mesenchymal stem cell/degradable hydrogel composite material as well as preparation method and application thereof
  • Bone marrow mesenchymal stem cell/degradable hydrogel composite material as well as preparation method and application thereof
  • Bone marrow mesenchymal stem cell/degradable hydrogel composite material as well as preparation method and application thereof

Examples

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Embodiment 1

[0049] In order to verify the existence form, mechanical strength and toxicity of the hydrogel of the present invention, this example prepares an active oxygen responsive degradable hydrogel material, which is the bone marrow mesenchymal stem cell / degradable hydrogel material provided by the present invention Composites without bone marrow mesenchymal stem cells. It includes a biocompatible hyaluronic acid component and an active oxygen-responsive hyperbranched polymer component, the hyaluronic acid component is obtained by modifying hyaluronic acid with a number average molecular weight of 100,000 through methacrylate, The active oxygen-responsive hyperbranched polymer is prepared by Michael addition reaction of polyethylene glycol diacrylate and ROS-responsive thiol diamine. Wherein, the molecular weight of polyethylene glycol diacrylate is 575. The number average molecular weight of the synthesized active oxygen responsive hyperbranched polymer HBPAK is 6000Da; the molecul...

Embodiment 2

[0060] This example prepares a bone marrow mesenchymal stem cell / degradable hydrogel composite material, which includes a biocompatible hyaluronic acid component, an active oxygen responsive degradable hyperbranched polymer component, and a cell adhesion peptide IKVAV, cell proliferation growth factors basic fibroblast growth factor bFGF and epidermal growth factor EGF, and bone marrow mesenchymal stem cells. Wherein, the hyaluronic acid component is obtained by modifying hyaluronic acid with a number average molecular weight of 120,000 through methacrylate, and the active oxygen-responsive hyperbranched polymer is made of polyethylene glycol diacrylate and ROS-responsive ketal thiol diamine prepared by Michael addition reaction. Wherein, the molecular weight of polyethylene glycol diacrylate is 575Da. The number-average molecular weight of the synthesized active oxygen-responsive degradable hyperbranched polymer HBPAK is 6000Da; the synthesized methacrylate-modified hyaluron...

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Abstract

The invention discloses a bone marrow mesenchymal stem cell / degradable hydrogel composite material as well as a preparation method and application thereof. According to the material, a polyethylene glycol derivative and active oxygen responsive micromolecular diamine are used as raw materials to prepare an active oxygen responsive polyethylene glycol hyperbranched polymer which is used as a component of hydrogel, and methacrylate modified hyaluronic acid is used as another component; cell adhesion peptides, basic fibroblast growth factors and epidermal growth factors are used as bioactive components, bone marrow mesenchymal stem cells are loaded in the bioactive components, and a method of crosslinking gel formation initiated by ultraviolet irradiation radiation is adopted to prepare the bone marrow mesenchymal stem cell gel. The prepared bone marrow mesenchymal stem cell / hydrogel composite material has good biocompatibility, a material system and degradation products are harmless to a human body, the mechanical modulus is moderate, no toxic or side effect is caused to embedded cells, and the bone marrow mesenchymal stem cell / hydrogel composite material can be widely applied to the fields of biological medicine, tissue engineering, repair and regeneration and the like and has good application prospects. The method is especially suitable for treatment of diseases such as spinal cord injury and nerve regeneration.

Description

technical field [0001] The invention relates to a bone marrow mesenchymal stem cell / degradable hydrogel composite material and its preparation method and application, belonging to the technical field of biomedical materials. Background technique [0002] The public pays more and more attention to their own medical health, and there is a growing demand for the treatment of various diseases and the recovery or reconstruction of body functions. With the continuous in-depth research on the pathogenesis of various diseases, the academic community has gradually realized that the onset of most diseases is highly related to the excessive inflammation of body tissues, and with the generation and uncontrolled development of inflammation, the microenvironment of body tissues occurs. Severe changes, especially oxidative stress. Specifically, during oxidative stress, the excessive production of reactive oxygen species (ROS) by inflammatory cells such as macrophages and neutrophils will ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/20A61L27/18A61L27/22A61L27/50A61L27/52A61L27/54A61L27/58C08B37/08C08G83/00
CPCA61L27/3834A61L27/3878A61L27/20A61L27/18A61L27/227A61L27/50A61L27/52A61L27/54A61L27/58C08B37/0072C08G83/005A61L2430/32A61L2300/414A61L2300/252A61L2300/412C08L5/08C08L71/02C08L89/00
Inventor 高长有李梓铭丁洁胡海军
Owner ZHEJIANG UNIV
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