Efficient phosphate-solubilizing bacterium MQR6 as well as fermentation product and application thereof

A technology of fermentation products and phosphate-dissolving bacteria, which is applied in the field of microbiology to achieve the effects of improving the utilization rate of phosphate fertilizer, promoting plant growth, and promoting root growth

Pending Publication Date: 2022-03-11
中国林业科学研究院华北林业实验中心
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the bottleneck restricting the development of my country's microbial fertilizer industry is the selection of high-efficiency strains

Method used

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  • Efficient phosphate-solubilizing bacterium MQR6 as well as fermentation product and application thereof
  • Efficient phosphate-solubilizing bacterium MQR6 as well as fermentation product and application thereof
  • Efficient phosphate-solubilizing bacterium MQR6 as well as fermentation product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Embodiment 1, the isolation and identification of phosphate-solubilizing bacteria MQR6

[0058] 1. Isolation of phosphate-solubilizing bacteria MQR6

[0059] Phosphorus-solubilizing bacteria MQR6 was isolated from the rhizosphere soil of Maple indica, and the sampling location was the National Long-term Research Base of Warm Temperate Forest in Jiulongshan, Beijing. The geographical coordinates are 115°59′~116°06′ east longitude, 39°54′~39°57 north latitude '. Nine sampling points were set up in the sample field by the S-shaped sampling method, and the 0-20cm soil was drilled with a soil drill, and the soil was mixed into a sterilization bag and brought back to the laboratory. According to the conventional separation method, apply and separate on the inorganic phosphorus solid medium, culture at 30°C for 48 hours, select the strain with a large clear transparent circle, and perform streak separation and purification on the inorganic phosphorus solid medium to obtain pu...

Embodiment 2

[0081] Embodiment 2, the physiological and biochemical characteristics of phosphate-solubilizing bacteria MQR6

[0082] 1.1 Determination of salt tolerance: Inoculate the same concentration of phosphate-solubilizing bacteria MQR6 bacterial solution (1% inoculum size) in YWA liquid medium containing 0%, 1.0%, 2.0%, 4.0%, 6.0%, 8.0%, 10.0% NaCl , v / v), cultured at 30°C for 7 days with shaking, and measured the absorbance at 600nm with a UV-1800 ultraviolet spectrophotometer. Growth salt concentrations ranged from 0-4.0%.

[0083] 1.2 pH range measurement: prepare phosphate buffer solution (solution A: 8.3ml phosphoric acid, add deionized water and mix to 500ml; solution B: Na 2 HPO 4 14.326g, mixed with deionized water and settled to 500ml; take 72.5ml of solution A and 27.5ml of solution B and mix, pH is about 2.0), acetic acid-sodium acetate buffer system (0.2mol / L acetic acid and 0.2mol / L sodium acetate For preparation, the pH value is about 5.0-6.0), potassium dihydrogen ...

Embodiment 3

[0085] Example 3, Qualitative detection and quantitative analysis of auxin secreted by Phospholyticum MQR6

[0086] According to the reference (Glickmann, E. Dessaux, A. critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Applied and environmental microbiology 1995, 61 (2), 793-796) described Salkowski colorimetric assay Phospholytic bacterium MQR6 secretes growth hormone IAA performance. Phosphorus solubilis MQR6 was inoculated in TSB liquid medium, shaken at 30°C and 180 rpm for 4 days to obtain fermentation broth of phosphate solubilizer MQR6. Using the uninoculated TSB liquid medium as a blank control, the OD600nm value of the phospholytic bacteria MQR6 fermentation broth was measured, and the results showed that the OD600nm value of the phospholytic bacteria MQR6 fermentation broth was 1.78 (this value is the OD600nm value of the blank control).

[0087] Qualitative detection is performed as follows...

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Abstract

The invention discloses an efficient phosphate solubilizing bacterium MQR6 as well as a fermentation product and application thereof. The name of the phosphate-solubilizing bacteria is (Pantoea) MQR6, the registration number of the phosphate-solubilizing bacteria in the China General Microbiological Culture Collection Center is CGMCC No.23609, and the nucleotide sequence of the phosphate-solubilizing bacteria is as shown in SEQ ID No.1. In the invention, the phosphate solubilizing bacteria (Pantoea) MQR6 is obtained by autonomously separating from soil and screening. Experiments prove that the phosphate solubilizing bacteria MQR6 can generate IAA, and each milliliter of fermentation liquor with the OD600nm value of 1 contains 165.52 micrograms of IAA. The strain has strong phosphate solubilizing ability, can activate insoluble phosphorus such as calcium phosphate, aluminum phosphate or iron phosphate in a short time, and can significantly improve the content of available phosphorus. The strain can secrete siderophores and is high in activity. The plant growth promoting agent is used for plant seedlings, has a remarkable growth promoting effect, improves the plant height and ground diameter growth amount of the seedlings, promotes and strengthens roots and improves the nutrient utilization rate. The strain is high in adaptability, is suitable for being applied to agricultural production as a microbial agent, a bacterial fertilizer and a soil conditioner, and has a wide application prospect.

Description

technical field [0001] The invention relates to the field of microbiology, in particular to a high-efficiency phosphate-dissolving bacterium MQR6 and its fermentation product and application. Background technique [0002] Phosphorus is a large amount of elements necessary for plant growth and development. Phosphorus is an important component of organic compounds such as nucleic acids, nucleoproteins, phospholipids, and phytophytes. It participates in energy metabolism, photosynthesis, respiration, glycolysis, redox reactions, and signaling in plants. Transduction and other physiological and biochemical processes. There is a large amount of phosphorus in the soil, but about 95% of it cannot be directly absorbed and utilized by plants. Insufficient phosphorus supply is often one of the important reasons restricting plant growth and development. The seasonal utilization rate of phosphate fertilizer is only 10% to 25%. Excessive application of phosphate fertilizer causes proble...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05G3/00C05G3/80A01N63/20A01P21/00C09K17/14C12R1/01C09K101/00
CPCC12N1/20C05F11/08C05G3/00C05G3/80A01N63/20C09K17/14C09K2101/00
Inventor 马庆华栾明宝王兴红原伟杰蔡京艳孔斌李建波
Owner 中国林业科学研究院华北林业实验中心
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