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Preparation method and application of double-enzyme active antibacterial material

An antibacterial material and a technology for enzymatic activity, which are applied to the preparation of double-enzyme active antibacterial materials and their application fields, can solve problems such as the unsatisfactory antibacterial effect of glucose oxidase, and achieve inhibition of bacterial growth, easy availability of preparation equipment, and application of promising effect

Pending Publication Date: 2022-03-18
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the bacteriostatic effect of a single glucose oxidase is not ideal, and it is necessary to seek a dual-enzyme activity bacteriostatic material with simple steps and no need to add hydrogen peroxide from an external source.

Method used

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  • Preparation method and application of double-enzyme active antibacterial material
  • Preparation method and application of double-enzyme active antibacterial material
  • Preparation method and application of double-enzyme active antibacterial material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The Fe of this embodiment 2 (MOO) 4 ) 3 The preparation method of @gox bisase active antibacterial material, the specific process steps are:

[0028] Molybdenum (FE) 2 (MOO) 4 ) 3 1.0 mg was dispersed in acetate buffer in pH = 4, and 1 mg of glucose oxidase was added, and 200 r / min was stirred at normal temperature for 10 minutes, then centrifuged at 5000 r / min for 5 min, collect precipitate, -40 ° C, freeze drying 24h , To obtain bindozyme-active antibacterial materials (Fe 2 (MOO) 4 ) 3 @Gox).

[0029]The method of preparing the molybdate iron according to the present embodiment is:

[0030] (1) The iron salt and molybdate are dissolved in two parts of the same volume of deionized water, the molar ratio of the iron salt and the molybdate is 1: 1.5, and then the molybdate solution is added dropwise to the iron salt solution. Then, the end reaction system was adjusted to pH = 3, and then at normal temperature of 200C / min for 30 min, transferred to 100 ml of the reacti...

Embodiment 2

[0035] The Fe of this embodiment 2 (MOO) 4 ) 3 The preparation method of @gox bisase active antibacterial material, the specific process steps are:

[0036] Molybdenum (FE) 2 (MOO) 4 ) 3 1.0 mg was dispersed in acetate buffer in pH = 4, and then 1.0 mg of glucose oxidase was added, stirred at normal temperature for 10 min, then centrifuge at the rotational speed of 8000r / min, collected from the precipitate, and frozen at -50 ° C Dry 36 h to obtain bindic acid-boridal antibacterial materials (Fe 2 (MOO) 4 ) 3 @Gox).

[0037] The method of preparing the molybdate iron according to the present embodiment is:

[0038] (1) The iron salt and molybdate are dissolved in two parts of the same volume of deionized water, the molar ratio of the iron salt and the molybdate is 1: 1.5, and then the molybdate solution is added dropwise to the iron salt solution. The final reaction system was then adjusted to pH = 3, and then 300r / min was stirred at normal temperature for 30 min, transferred t...

Embodiment 3

[0041] This embodiment relates to Fe 2 (MOO) 4 ) 3 @GOX In vitro Antibacterial application experiment, the Fe prepared in Example 1 2 (MOO) 4 ) 3 @GOX is applied to in vitro antibacterial experiments, and the solid LB medium is vaccined to 50 ml of sterile liquid LB medium (containing trypsin (0.5 g) of the S. cells. , Yeast extract (0.25 g) and NaCl (0.5 g)), then placed the suspension of the resistive bacteria to cultivate overnight at 37 ° C on the rotary oscillator; then dilute bacteria with sterile PBS. 10 6 CFU / ml, the bacterial solution (100 μm) and 200 microliters of mass concentration is 60 μg · ml -1 FE 2 (MOO) 4 ) 3 The @GOX aqueous solution and 700 microliters of PBS buffer were incubated at 37 ° C for 30 min, and then 50 microliters of the above mixed solution was uniformly applied to solid Lb medium, and the solid Lb medium was cultured in an incubator of 37 ° C. In 12 hours, the bacterial colonies were counted by CFU method; PBS was used as a blank control, and th...

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Abstract

The invention belongs to the technical field of antibacterial material preparation and application, and relates to a preparation method and application of a double-enzyme active antibacterial material, and the specific process steps are as follows: dispersing iron molybdate in an acetate buffer solution with the pH value of 4, adding glucose oxidase, stirring for 10 minutes at normal temperature at 200-300r / min, then centrifuging, collecting precipitate, and freeze-drying to obtain the double-enzyme active antibacterial material. The double-enzyme activity antibacterial material Fe2 (MoO4) 3 (at) GOx of the iron molybdate loaded glucose oxidase is obtained; fe2 (MoO4) 3 (at) GOx has excellent peroxidase and glucose oxidase activity and can reduce the damage of exogenous hydrogen peroxide to tissues in bacteriostatic application, and a hunger therapy generated by glucose oxidase can cooperate with active oxygen generated by iron molybdate to efficiently resist bacteria; the preparation method is simple and has efficient antibacterial performance, the whole process is green, environment-friendly and pollution-free, and the application prospect is wide.

Description

Technical field: [0001] The present invention belongs to the preparation and application of the antibacterial material, which relates to a double enzymatic activity. 2 (MOO) 4 ) 3 @GOX material preparation method and application, synthesized molybdenate iron by hydrothermal, using electrostatic adsorption, glucose oxidase, the hunger therapy produced by synergistosidase and the active oxygen generated by molybdate to achieve high efficiency antibacterial The purpose, the antibacterial effect is good and is harmless to the human body. Background technique: [0002] Diabetes is currently a global epidemic, and the number of patients is increasing every year. It has become one of the most prominent public health threats, affecting about 425 million people around the world. Diabetic foot ulcer is also known as diabetic limbs, is one of the most severe chronic complications of diabetes, and is also an important reason for the disability and death of diabetic patients. Diabetes patient...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/55A61K33/26A61K38/44A61P31/04C01G49/00
CPCA61K47/55A61K33/26A61K38/443C12Y101/03004A61P31/04C01G49/00C01P2004/03C01P2004/04C01P2004/01A61K2300/00Y02A50/30
Inventor 牛玉生许元红张彦芳
Owner QINGDAO UNIV
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