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Method for evaluating toxic effect of petroleum organisms with control stations based on Sebastiscus marmoratus

A technology of biotoxicity and scorpionfish, applied in the field of assessing the biological toxicity effects of petroleum at control sites based on scorpionfish, can solve problems such as unavailable offshore oil

Pending Publication Date: 2022-03-18
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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Problems solved by technology

[0006] The object of the present invention is to provide a method for assessing the petroleum biotoxicity effect of the control station based on the brown calamari, to solve the problem that there is no quantitative assessment of the offshore petroleum biotoxicity effect of the control station based on IBRv2 in the above-mentioned background technology The problem

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  • Method for evaluating toxic effect of petroleum organisms with control stations based on Sebastiscus marmoratus
  • Method for evaluating toxic effect of petroleum organisms with control stations based on Sebastiscus marmoratus
  • Method for evaluating toxic effect of petroleum organisms with control stations based on Sebastiscus marmoratus

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Embodiment Construction

[0026] The technical solutions of the present invention will be further explained below through specific examples.

[0027] The method of assessing the biotoxicity effect of oil at a control station based on the brown calamari in the embodiment of the present invention was carried out in Maowei Sea, Guangxi in April 2017. Maowei Sea is located at the top of Qinzhou Bay, Guangxi, and is a semi-enclosed inner bay with a sea area About 135km 2 , the average water depth is less than 10m. In recent years, with the concentration of heavy industries such as petroleum refining in Qinzhou's coastal areas, Maowei Sea has been increasingly threatened by petroleum pollution. Pollutants such as polycyclic aromatic hydrocarbons and polychlorinated biphenyls have been detected in recent years. The method includes the following steps:

[0028] Step 1: Using the in-situ monitoring experiment in the sea area, set up several oil pollution monitoring stations and a clean control station; in thi...

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Abstract

The invention relates to a method for evaluating the toxic effect of petroleum organisms with control stations based on Sebastiscus marmoratus, which comprises the following steps: (1) setting a plurality of petroleum pollution monitoring stations and a clean control station by adopting a sea area in-situ monitoring experiment; (2) hanging healthy sebastiscus marmoratus at different monitoring stations by using net frames; (3) monitoring lasts for 14 days, the survival condition of the sebastiscus marmoratus is observed on the last day, and liver, gallbladder and gonad tissues of the survival sebastiscus marmoratus are taken for physiological and biochemical detection; (4) dividing the marine petroleum biological toxic effect into five levels; (5) based on the grading coefficient of each molecular biomarker and the response index of the second-generation comprehensive biomarker, obtaining grading thresholds from first grade to third grade; and (6) analyzing and evaluating the risk level of the toxicity effect of the offshore oil type organisms with the control stations. The method has the advantages of simplicity, wide raw material source, low cost, easiness in operation, remarkable effect and the like, and can quantitatively evaluate the safety of marine organisms and ecological systems of offshore oil pollution.

Description

technical field [0001] The invention relates to the technical field of offshore oil pollution assessment, in particular to a method for assessing oil biotoxicity effects at control stations based on scorpionfish. Background technique [0002] Petroleum is not only one of the most important pollutants in the marine environment in the world but also in my country. It mainly comes from industrial emissions, shipping, offshore oil exploration and oil spill accidents. In recent years, although the rapid spread of marine pollution in my country has been slowed down to a certain extent, oil pollution in some sea areas is still serious. It can be predicted that with the rapid development of my country's industrial and agricultural production, the acceleration of urbanization, the continuous development of maritime transportation and fishing, the rapid development of offshore oil exploration and development, and the frequent occurrence of oil spills, petroleum will still be the larg...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/28G01N21/64
CPCG01N33/2835G01N21/6428G01N21/6486
Inventor 郑榕辉薄军方超
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES