Recombinant adenovirus ADV-lncRNA Gm44275 as well as construction method and application thereof

A technology of adv-lncrnagm44275 and recombinant adenovirus, which is applied in the direction of recombinant DNA technology, viruses, applications, etc., can solve the problems affecting meat food safety, exogenous hormone residues, and high requirements for precise delivery control, etc., to achieve precise control of female animal delivery , individual differences have little influence, and the effect is stable

Pending Publication Date: 2022-03-22
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to the different injection volumes of different individuals, there will still be more cases of late or delayed delivery. The requirements for

Method used

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  • Recombinant adenovirus ADV-lncRNA Gm44275 as well as construction method and application thereof
  • Recombinant adenovirus ADV-lncRNA Gm44275 as well as construction method and application thereof
  • Recombinant adenovirus ADV-lncRNA Gm44275 as well as construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1LNCRNA constructs of overexpression vectors

[0045] Long chain non-coding RNA (Long Noncoding RNA, LNCRNA) refers to a non-encoded RNA having a length greater than 200 nucleotides. The non-coded RNA plays an important role in an apparent genetic regulation, a cell cycle regulation, and cell differentiation, etc., and some LNCRNA has tissue and spatial specificity. The present invention first synthesizes LNCRNAGM44275 (LNCRNA GM36582) by in vitro synthesis, and sequence sequence sequence sequence (sequence sequence sequence sequence sequence sequence) and green fluorescent label sequence (selected) is then added to the LNCRNA GM44275 sequence. See SEQ ID No. 3) to obtain a lncRNA overexpression vector, which sequence seq ID No.4.

Embodiment 2

[0046] Example 2ADV packaging lncRNA overexpression vector

[0047] Adenovirus (ADV) refers to a macromolecule (36 kB) double-strandless DNA virus. The adenoviral genome was transferred to the nucleus through the receptor-mediated endocytosis. The inactivated adenovirus can be integrated as a carrier, injecting the target gene fragment, injecting the human or animal, promoting the expression of the gene.

[0048] Padeno vecotr vector is an adenovirus expression vector, carrier structure mode such as figure 1 Indicated. The above-mentioned LNCRNA overexpression vector is homologously recombinant in vitro to the Padeno vecotr vector, and the LNCRNAGM44275 (LNCRNA GM36582) sequence is transferred to the Padeno Vecotr vector to give GM44275-CBH-GFP adenovirus plasmid.

[0049] The plasmid was linearized by PACI, and the enzyme digestion was transfected into HEK293 cells, and when the cells had a pathological reaction and 50% left and right cells fall off, lysed cells were collected by...

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Abstract

The invention discloses a recombinant adenovirus ADV-lncRNA Gm44275 as well as a construction method and application thereof, and relates to the field of induced delivery. The recombinant adenovirus ADV-lncRNA Gm44275 is prepared by the following steps: adding a CBH Promoter sequence and a green fluorescent label sequence into an lncRNA Gm44275 sequence to construct an lncRNA overexpression vector, then carrying out homologous recombination on the lncRNA overexpression vector and an adenovirus expression vector, and carrying out enzyme digestion, transfection, purification and concentration, thereby obtaining the recombinant adenovirus ADV-lncRNA Gm44275. The recombinant adenovirus ADV-lncRNA Gm44275 can be applied to preparation of a medicine with a function of inducing animal delivery. According to the present invention, the feed can promote the early delivery of female animals, does not affect the health of the female animals, has characteristics of more universality, no hormone residue in the animal body when the feed is used for various animals, stable delivery induction effect, and less influence by individual differences.

Description

Technical field [0001] The present invention relates to the field of inducing delivery, and more particularly to a recombinant adenovirus ADV-LNCRNA GM44275 and its construction methods and applications. Background technique [0002] Induced delivery refers to human intervention leads to the occurrence of childbirth. Induction of childbirth during animal production can improve the efficiency of reproduction, which can promote the production of animal animals, centralized delivery is conducive to the management of populations, which can make daily management, centralize, controlled, can save labor costs, or It is convenient for health management to animals, improves self-contained activity, and increases production benefits, and has significant economic value. [0003] Currently, there are several ways to induce childhood induction childbirth: [0004] (1) sow induced childbirth [0005] Prerenergic analogs (such as chlorine, oleanol, sodium chlorine, sodium chloride, etc.) are of...

Claims

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Application Information

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IPC IPC(8): C12N7/01C12N15/861C12N5/10C12N15/64A61K31/7088A61P15/04
CPCC12N7/00C12N15/86C12N5/0686C12N15/64A61K31/7088A61P15/04C12N2510/02C12N2710/10021C12N2710/10043C12N2710/10052C12N2710/10031
Inventor 周虚李纯锦赵子娇陈璐赵云
Owner JILIN UNIV
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