Detection test strip for detecting antistreptolysin O antibody

A technology for detecting streptolysin and test strips, which is applied in the field of medical biological detection, and can solve the problems of red blood cell instability, long time-consuming, and complicated operation of the measurement process.

Pending Publication Date: 2022-03-25
SHANGHAI JIEMEN BIO TECH
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

Due to the instability of human or animal red blood cells, the hemolysin inhibition method has different sensitivity to hemolysin, and the operation is cumbersome, the measurement results are easily affected, and the repeatability is poor.
The latex agglutination method uses the mixture of serum and latex reagent, and the results are judged by the naked eye according to whether there is agglutination. This method is susceptible to human factors and external influences, and can only be used for qualitative detection.
The enzyme-linked immunoassay assay process is complicated and time-consuming, which is not conducive to clinical promotion and application, and is not suitable for screening and testing of emergency samples and large-scale samples

Method used

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  • Detection test strip for detecting antistreptolysin O antibody
  • Detection test strip for detecting antistreptolysin O antibody
  • Detection test strip for detecting antistreptolysin O antibody

Examples

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Embodiment 1

[0148] This embodiment 1 provides an immunochromatographic test strip and its use for measuring anti-streptolysin O antibody (ASO).

[0149] "SLO" refers to Streptolysin O.

[0150] "ASO" refers to Anti-Streptolysin O antibody (Anti-Streptolysin O).

[0151] 1. Conjugate pad preparation:

[0152] 1.1 Preparation of fluorescent microsphere solution

[0153] 1.1.1 Take 1ml of fluorescent latex particle solution (containing carboxyl groups) (Shanghai Shuoyuan Biotechnology Co., Ltd., 0.07μm particle size), add 1ml of water and 2ml of 0.05M pH 6.5 PBS buffer, and sonicate on an ultrasonic cell disruptor to fully Disperse the microspheres to obtain a fluorescent microsphere dispersion.

[0154] 1.1.2 Take 50μl EDC and add it to the fluorescent microsphere dispersion under stirring, and activate after mixing for 10 minutes at 25°C. The precipitate obtained by centrifugation is dispersed with 1ml 0.05M pH 8.4 PBS buffer solution, and ultrasonicated on an ultrasonic cell pulverizer...

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Abstract

The invention relates to a test strip for detecting an anti-streptolysin O antibody. Specifically, the invention provides an antistreptolysin O antibody detection test strip, the test strip comprises a substrate, and a sample pad, a combination pad, a coating film and a water absorption pad which are positioned on the substrate and are sequentially arranged along the direction from the near end to the far end of the test strip; the combination pad comprises fluorescent microspheres loaded with streptolysin O; the coating film comprises a detection line and a quality control line, the detection line comprises streptolysin O, and the quality control line comprises streptolysin O. The detection test strip disclosed by the invention can be used for rapidly and simply determining the anti-streptolysin O antibody in a sample to be detected.

Description

technical field [0001] The invention relates to the technical field of medical biological detection, in particular to a test strip for detecting anti-streptolysin O antibodies. Background technique [0002] Anti-Streptolysin O antibody (Anti-Streptolysin O, referred to as ASO), is one of the metabolites of group A streptococcus in the human body. It is a protein containing -SH group and has hemolytic properties. ASO can dissolve red blood cells , easy to be oxidized in the air, after oxidation, it can temporarily lose the hemolytic ability, and can recover the hemolytic ability after being reduced by a reducing agent. After the human body is infected with hemolytic streptococci, a large number of anti-streptolysin O antibodies may appear in the serum, thereby increasing the concentration of anti-streptolysin O in the blood. The determination of ASO is valuable for the diagnosis of group A streptococcal infection, and its presence and content can reflect the severity of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/533G01N33/543G01N33/531G01N33/558G01N21/76
CPCG01N33/6854G01N33/533G01N33/54313G01N33/531G01N33/558G01N21/76G01N2469/20G01N2333/315
Inventor 马佳琪娄珺顾晓敏徐蕊萍
Owner SHANGHAI JIEMEN BIO TECH
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